Japanese Journal of Medical Science and Biology
Online ISSN : 1884-2828
Print ISSN : 0021-5112
ISSN-L : 0021-5112
Search
Advanced search
OR
Browse
Search
Volume 50, Issue 4-5
Displaying 1-5 of 5 articles from this issue
Show all abstracts
    • |<
    • <
    • 1
    • >
    • >|
  • SCREENING OF HUMAN CORNEAS FOR HERPES SIMPLEX VIRUS BY TISSUE CULTURE AND POLYMERASE CHAIN REACTION
    Emmanuel E. BINEY, Fitzroy A. ORRETT
    1997 Volume 50 Issue 4-5 Pages 151-160
    Published: 1997
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.50.151
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Superficial eye infections by herpes simplex virus (HSV) constitute a major cause of corneal disease, necessitating the need for corneal transplantation in many patients. Eighty-three corneas from 46 post-mortem donors received from the David Lucas Eye Bank in Manchester were analyzed by Vero cell culture and the polymerase chain reaction (PCR) technique to detect HSV. There was no evidence of a characteristic cytopathic effect in any of the cultures. A 350-bp PCR product corresponding to the HSV thymidine kinase (TK) was detected by Southern blotting in only 2.4% (2/83) of samples. In contrast, approximately 70% of samples yielded a 758-bp PCR product. Although this low prevalence of HSV in corneas may be encouraging, it is high for the actual transplantation program if the viral DNAs maintain their abilities to replicate.
    View full abstract
    Download PDF (4772K)
  • DETECTION OF HeLa CELL CONTAMINATION-PRESENCE OF HUMAN PAPILLOMA VIRUS 18 DNA AS HeLa MARKER IN JTC-3, OG AND OE CELL LINES
    Hajime OGURA, Ritsushi FUJII, Masako HAMANO, Mitsutaka KUZUYA, Hiroshi ...
    1997 Volume 50 Issue 4-5 Pages 161-167
    Published: 1997
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.50.161
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    There are warnings of the contamination of cell cultures with HeLa cells in many laboratories in the world. The cell lines JTC-3, OG and OE that were estabilished in Okayama in 1959, 1969 and 1971, respectively, were examined for human papillomavirus (HPV) 18 DNA by Southern blot hybridization. The HPV 18 DNA detected in these three cell lines showed hybridization patterns characteristic of the HPV 18 DNA in the HeLa cell line established in 1951. Southern hybridizaiton patterns of HPV 18 DNA in the cellular DNA of the C4-II cervical cancer cell line that was established in the USA in 1962 was different from that of HeLa cells. These results suggest that the JTC-3, OG and OE cell lines have been contaminated by HeLa cells.
    View full abstract
    Download PDF (6498K)
  • SUBCELLULAR LOCALIZATION OF HEPATITIS C VIRUS STRUCTURAL PROTEINS IN THE LIVER OF TRANSGENIC MICE
    Kyoji MORIYA, Hajime FUJIE, Hiroshi YOTSUYANAGI, Yoshizumi SHINTANI, T ...
    1997 Volume 50 Issue 4-5 Pages 169-177
    Published: 1997
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.50.169
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Hepatitis C virus (HCV) core and envelope proteins are suggested to be responsible for the pathogenesis of hepatic and extrahepatic manifestations in chronic hepatitis C. Moreover, the core protein is implicated in the regulation of the transcription of cellular genes including c-myc, RB and p53. Determining the subcellular localization of the core and envelope proteins is therefore necessary to elucidate their behaviors, particularly in vivo ones, regarding the interaction with transcriptional regulatory proteins or gene elements. We defined the subcelluar localization of HCV envelope and core proteins which were expressed in substantial levels in the liver of transgenic mice. Subcellular fractionation by ultra-centrifugation revealed that the envelope proteins were present principally in the microsomes of the liver, while a small amount of the protein was detected also in the nuclei. Immunohistochemistry confirmed the localization of envelope proteins in the nuclei. In contrast, the core protein was detected principally in the cytoplasmic fraction, where it was closely associated with lipids. A low level of the core protein was detected also in the nuclei and microsomal fraction. These results suggest possible interaction of the HCV structural proteins with some factors in hepatocytes thereby perturbing intracellular circumstances.
    View full abstract
    Download PDF (5021K)
  • SYMPOSIUM ON TOXINS
    1997 Volume 50 Issue 4-5 Pages 179-207
    Published: 1997
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.50.179
    JOURNAL FREE ACCESS
    Download PDF (2340K)
  • ERRATUM
    1997 Volume 50 Issue 4-5 Pages e1
    Published: 1997
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.50.4-5_e1
    JOURNAL FREE ACCESS
    Download PDF (38K)
    • |<
    • <
    • 1
    • >
    • >|
feedback
 Top