A highly sensitive procedure of solid-phase radioimmunoassay (RIA) was developed for the detection of measles IgG antibody. HeLa cells persistently infected with measles virus were used as a solid-phase antigen. This technique was applied to the detection of measles IgG antibody in patients with subacute sclerosing panencephalitis (SSPE) and multiple sclerosis. Normal subjects having experienced natural measles or measles vaccination and patients with various neurological diseases of non-virus nature were also examined as control groups. Measles antibody was detected at high titers in both the sera and cerebrospinal fluid of SSPE patients. Moreover, RIA/HI ratios of SSPE patients were significantly higher than those of normal subjects, suggesting the presence in the formers of antibodies to nucleocapsids at high titers as well as to viral envelopes. On the other hand, no significant difference was found in both RIA and HI titers between the sera of multiple sclerosis and those of various neurological diseases.

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Effects of long day and short day treatments during the embryonic and larval stages on induction of pupal diapause were studied on a diapausing race of Sarcophaga peregrina. Two long day (15L 9D) cycles during 2 days before or after the larviposition completely stopped the induction of pupal diapause on larvae which grow in short day condition before and after the long day treatment.
The sensitivity appeared to decrease during the early stage of the third instar and to increase again to some extent in the prepupal stage.

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