ホンシメジの栄養生長における栄養要求性を基礎合成培地を設定して静置培養法により検討した．グルコース，キシロース，マンノース，フルクトース，スクロース，デキストリン，グリコーゲン，ペクチン，可溶性デンプンは良好に資化されたが，セルロース，リグニン，キチンは資化されなかった．ポリペプトン，ソイトン，酵母エキス，肉エキス，カザミノ酸およびアミノ酸混合物は良好な窒素源であり，アラニン，イソロイシン，バリン，グルタミン，シトルリン，セリンは単一でアミノ酸混合物に匹敵する効果を示した．しかし，無機態窒素での生長は良好ではなかった．燐酸カリウム，硫酸マグネシウムは栄養生長に不可欠であり，硫酸亜鉛，硫酸鉄も添加効果を示した．チアミン，ニコチンアミドは栄養生長に影響を与えたが，他のビタミン類，核酸関連物質には顕著な影響を示すものはみられなかった．植物ホルモン（IAA，KIN，GA₃，NAA，2,4-D）は生長促進効果を示した．

　Methylated saccharides from yeast cells were analysed by thin layer chromatography (TLC) using diatomaceous earth as a support. Residues from which fatty acids had been extracted for analysis of yeast cell constitution were used as samples. Freeze-dried yeast cells (50 mg) were hydrolysed with 5% methanolic hydrochloride acid, and fatty acid methyl ester was extracted from the hydrolate with n-hexane. Chloride ions were removed from the residual solution with ion exchange resin. The solution was concentrated and used as a sample. Thin layer plates were prepared with mixture of diatomaceous earth (Wako Pure Chemical Indus.) and plaster (9 : 1). A mixture of 67% isopropanol (35 ml) and ethyl acetate (65 ml) was used as the solvent for the one-step development. Anthrone-sulfuric acid reagent was used for detection. The Rf value (×100) of the light yellowish brown spot of xylose was 60. Mannose gave a dark brown major spot of Rf value 47 and a minor spot of Rf value 25, and glucose gave a bluish purple spot of Rf value 40. For galactose, two clear, light yellow spots (Rf value, 25 and 28) and two minor spots (Rf value, 45 and 55) were observed. The presence of minor spots indicating anomers of mannose and galactose did not disturb the detection of major spot of methylated saccharides in mixed solution. Mannose, glucose and galactose were detected in strains of Geotrichum, Kluyveromyces, Pichia and Schizosaccharomyces by this thin layer chromatography. Glucose and galactose were detected in the strain of Cryptococcus and Trichosporon, together with minor spots of xylose. The methylated saccharide mixture could not be separated on a thin layer plate prepared with Kieselgur G of Merck (Germany). This one-step TLC may be suitable to the case of a few samples whose constituent saccharides need to be known immediately, as it needs no multiple development.

Two taxa of Inocybe collected by Kumagusu Minakata were identified as I. glabrodisca and I. aff. dunensis, respectively, and are new to the Japanese mycoflora. Their microscopic features are reported with illustrations.

　菌床培地によるホンシメジの子実体形成条件を検討した．可溶性デンプン，グルコース，ペクチン，酵母エキス，KH₂PO₄，MgSO₄，チアミン，CaCO₃，粉炭，ピート・モス（支持体）を組成とする培養基を用い，23℃，90日間の培養・熟成後，16°C，30日間の発生操作により子実体は形成された．13-15日目に子実体原基が現われ，その後，14-17日目に子実体は成熟した．供試した27菌株の全てが子実体を形成し，1栽培袋当たりの収量は14.6-62.7 gであった．