1. Fresh crush preparations of the liver of Oryzias latipes were made and examined by means of phase contrast microscopy.
2. The nuclei in fresh preparations look homogeneous except the nucleoli, but with elapse of time the network appear.
3. In fresh preparations, several vacuoles are sometimes visible within the nucleolus.
4. The clearest picture of mitpchondria is seen with the dark contrast medium: in fresh preparations they are filamentous, in the older preparations they swell, shorten themselves forming nods.
5. The mitochondria outside of cells look like short swollen rods or granules.
6. The Golgi apparatus is demonstrated and distinguished from mitochondria with the dark contrast low.
The author expresses his heartiest t h a nks for the accurate criticism of Dr. Prof. T. Taniguchi.
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