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Volume 69 (1992 - 199･･･
- Issue 6 Pages 253-
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Volume 69, Issue 6
Displaying 1-13 of 13 articles from this issue
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Toshikazu NAGATO1993 Volume 69 Issue 6 Pages 253-263
Published: March 22, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSExocrine glands including the submandibular gland, sublingual gland, exocrine pancreas and exorbital lacrimal gland of newborn rats aged 0 to 14 days were examined morphologically, and the following results on the accumulation of lipid droplets were obtained. Lipid droplets tended to localize in secretory cells, especially in their basal cytoplasm. The degree of lipid droplet accumulation varied with the type of exocrine gland. There were large accumulations in the sublingual gland, submandibular gland, and exocrine pancreas, but accumulations were small in the exorbital lacrimal gland. No difference in lipid droplet accumulation was recognized between the 2 types of secretory cells in the sublingual gland. The accumulation of lipid droplets peaked 24-48 hours after birth in the sublingual gland, submandibular gland and exocrine pancreas, but this peak was not clearly observed in the exorbital lacrimal gland. In the group of newborn rats separated from their mothers and therefore not suckled, no lipid droplets were observed in any gland, suggesting a close relationship between lipid droplet accumulation and suckling.View full abstractDownload PDF (6017K) -
Takeshi NISHIMURA, Hitoshi OKUDA1993 Volume 69 Issue 6 Pages 265-275
Published: March 22, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSSuperior dental rami diverging from arterial multi-resources of the crab-eating monkey were investigated by means of plastic corrosion castings. Correlations between the dental ramus/rami entering the apical foramen/foramina of each maxillary tooth were elucidated. Superior dental rami supplying the maxillary teeth diverged from the posterior superior alveolar, infraorbital, descending palatine, major palatine and sphenopalatine arteries as follows: The posterior superior alveolar gave rise to molar branches which supplied three molars and rarely an additional two premolars, to premolar branches and to a canine branch as its terminus. The infraorbital gave of canine branches. The descending palatine gave rise to a molar branch which supplied the lingual root of each molar. The major palatine gave rise to a premolar branch which supplied the lingual roots of two premolars and the 1st molar, and an incisive branch which supplied two incisors. The sphenopalatine supplied the lingual root of each molar and rarely the 1st premolar in addition. Two incisors were supplied by the major palatine (100%), the canine by the posterior superior alveolar (50%), this artery and the infraorbital (43.3%) or the infraorbital only (6.7%). Three roots of each premolar were supplied by the posterior superior alveolar, occasionally the lingual root (16.7%) and mesiobuccal root (3.3%) of the 1st premolar by the major palatine, the lingual root of the 2nd premolar by the major palatine (6.7%) and the sphenopalatine (3.3%). Two buccal roots of each molar were also supplied by the posterior superior alveolar (100%), occasionally the lingual root of the 1st molar by the descending palatine (6.7%), the major palatine (6.7%) or the sphenopalatine (3.3%), the lingual root of the 2nd molar by the descending palatine (30%), the sphenopalatine (10.0%) or the infraorbital (3.3%), the lingual root of the 3rd molar by the descending palatine (23.3%), the sphenopalatine (20.0%) or the infraorbital (3.3%). Superior dental rami diverging from the posterior superior alveolar artery were distributed to the molars, premolars and canine as principal arterial vessels of the crab-eating monkey. The descending palatine, sphenopalatine and the infraorbital arteries were supplementary to the above main arteries. Two incisors were only supplied by the major palatine but the anterior and middle superior alveolar were not observed.View full abstractDownload PDF (4083K) -
Tsuyuka KUSHIDA, Yasukazu NAGATO, Haruyuki IIJIMA, Hiroshi KUSHIDA1993 Volume 69 Issue 6 Pages 277-287
Published: March 22, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSSemithin sections, cut from tissues stained with acid and basic dyes after embedding in 2-hydroxypropyl methacrylate, Quetol 523 and methyl methacrylate, showed cytoplasmic components at a high resolution by light microscopy. These same sections could then be viewed, after osmium tetroxide, uranyl and lead staining, by the electron microscope. These sections had a number of inherent advantages: they could be observed with a light microscope, they facilitated analysis of cellular structures in the identical sites, and they were frequently the optimum thickness to provide threedimensional information. We clearly established the structural detail of this same-section correlative light-electron microscopy approach by showing that the coloured materials observed in such sections of cells followed the distribution of fine structures within the same sections as determined by electron microscopy. In some instances the fidelity of the correlation between th e distribution of the coloured area and cytoplasmic components in identical cells of the same section revealed significant details which could not visualized in thin sections. This technique, therefore, provided a simple and useful solution to many problems that require the localization of cellular components in identical cells selected previously by light microscopy.View full abstractDownload PDF (3687K) -
Takayuki KANBAYASHI, Toyoharu TAKAFUJI, Yasushi SATO1993 Volume 69 Issue 6 Pages 289-310
Published: March 22, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe distribution of the arterial supply and innervation of skeletal muscle in the whole human body has been being researched in our laboratory. The results obtained on the biceps brachii from 25 bodies (50 sides) are reported here. Generally, in this muscle, A. coracobrachialis (Cb, SATO,1980), a branch of A. axillaris (Ax), is distributed at the upper muscle bundles of the short head after running parallel to N. musculocutaneus (Nmc) and supplying M. coracobrachialis. The main artery (Ma), which is a branch of A. brachialis (Br), is distributed at the upper and middle parts of both heads, and the point where Ma enters the muscle bundles almost coincides with the point where R. brachialis (Rb) branches from Nmc. R. biceps brachii (Rbi), which is distributed only at this muscle and Om, the common trunk to other muscles, is distributed at the lower part of the muscle bundles. The arterial supply of this muscle was classified as follows according to the conditions of origin and its distribution. Type I: Ax(Cb) was distributed to the upper part of the muscle bundle. Type H: The absence of Ax(Cb) in the upper part of the muscle bundle. Type A, a and 1: Ma. Br(Om) supplying the long, short and both heads of the biceps brachii. Type B, b and 2: Ma. Br(Rbi) supplying the long, short and both heads of the biceps brachii. In the long head, Type II-A accounted for 62.0%, Type II-B for 30.0%. In the short head, Type I-a was 62.0%, Type I-b 28.0%. In both heads, Type I-1 was 62.0%, Type I-2 28.0%. There were significant differences in the distribution of the nutrient arteries between the long and short heads. A. brachialis superficialis (Bs) was recognized in 3 of these cases (2 cases of Type III and 1 case of Type VII by Adachi's classifications), which were rare cases. In these cases, arterial branches other than Cb branching from A. brachialis profunda are branches of Bs. This means, judging from ontogeny, that Bs, the blood vessels in the superficial layer, supplies this muscle, which belongs to the superficial layer of the brachium.View full abstractDownload PDF (7486K) -
Katsuhiro INOUE, Tornoichiro YAMAAI, Goroh ODAJIMA1993 Volume 69 Issue 6 Pages 311-314
Published: March 22, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSA ease of a persistent sciatic artery in a 60-year-old Japanese man is reported. The right persistent sciatic artery (5 mm in diameter) was joined to the internal iliac artery proximally and to the popliteal artery distally. The terminal vessel of the femoral artery (3 mm in diameter) was connected to the sciatic artery at the popliteal fossa.View full abstractDownload PDF (1936K) -
Takashi UEDA, Satoshi TERADA, Osamu FUJIMORI, Kazuyori YAMADA1993 Volume 69 Issue 6 Pages 315-319
Published: March 22, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSA dual staining method was established for the histochemical detection of neutral carbohydrates and deoxyribonucleic acid (DNA) in light microscopy. The method consisted of combined periodic acid-thiocarbohydrazide-silver protein-physical development (PA-TCH-SP-PD) and hot hydrochloric acid (H-HCI)-Schiff (Feulgen) procedures, which produced reaction products of blackish and magenta shades respectively. The present dual staining method is believed to be unusually useful for the light microscopic histochemical studies on neutral carbohydrate-containing cells, since it exhibits a high contrast between these two shades and reveals distinctly the localization of cytoplasmic neutral carbohydrates and nuclear DNA in one and the same tissue section.View full abstractDownload PDF (1889K) -
Atsushi MORIYA, Toyoharu TAKAFUJI, Yasushi SATO1993 Volume 69 Issue 6 Pages 321-333
Published: March 22, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSIn the following, we report our findings obtained as a result of injecting an acrylic pigment in the arteries supplying the pectoralis minor muscle in 50 lateral chests of 26 Japanese adults (15 males and 11 females). In the pectoralis minor muscle, the muscular bundle near the terminal is supplied by A. processus coracoideris (Pc, Sato-Takafuji, '85) or A. coracobrachialis (Cb, Sato, '80) of A. axillaris (Ax), while its middle upper and lower peripherals are supplied by A. thoracoacromialis (Ta) and A. partis abdominalis (Pab, Sato, '76), respectively. Further, the upper and lower peripherals at its origin are supplied by A. thoracica suprema (Ts) and A. thoracica lateralis (Tl), respectively. Pc, Cb, Pab and Ts may occasionally be absent. Arteries supp lying this muscle are classified according to their origins and routes of distribution, as follows. Type I-a: Pc or Cb, Ta, Pab, Ts and Tl are present,32%; Type II-a: Ts is absent from Type I-a,14%; Type III-a: Pb and Pc are absent from Type I-a,20%; Type IV-a: Cb, Pc and Ts are absent from Type I-a,10%. Type b is Type a without Pab. The rates of appearance of Type I-b, II-b, III-b and IV-b were all 6%. The ratios of distribution in area a were as follows, in order of decreasing ratio: 37.6% for Pab (37 cases),32.4% for Tl(49 cases),30.2% for Ta (49 cases),10.8% for Ts (32 cases),7% for Cb (9 cases), and 6.37% for Pc. In the pectoralis minor muscle, the major supplying arteries are Pab, Tl and Ta, and where Pab was absent, this was compensated for by a branch of Ta. The total number of supplying arteries in this muscle was two to five, with the majority, or 36%, having four arteries. As for sex differences in the incidence of each type, Type I-a appeared more often in males (40%) than in females (20%). The rates according to the ribs of origin were 46%,36%,16%, and 2% for types 2-3-4-5,3-4-5,2-3-4, and 2-3-4-5-6, respectively. It was interesting that all Type II and Type IV cases without Ts corresponded to Type 3-4-5 without the muscular bundle arising from the second rib, as it suggests the process of degeneration of the pectoralis minor muscle in Homo. Innervating the pectoralis minor muscle, nerves containing fibers from the Truncus medius (mainly C7) were found in a small upper peripheral region, and those containing fibers from the Fasciculus medialis (C8, Th1) were distributed in the majority of the remainder in 50% of the subjects studied, while 36% lacked the branch from the Truncus medius. There was no strong correlation between the nerves and the type of arterial distribution.View full abstractDownload PDF (4397K) -
Seiji MATSUDA, Hiroko FUJITA, Shingi IMAOKA, Nobuaki OKUMURA, Masahiro ...1993 Volume 69 Issue 6 Pages 335-343
Published: March 22, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSDiscrepancies between previous light microscopic studies on the localization of basic fibroblast growth factor (bFGF) in neuroglias and neurons of the normal rat brain prompted us to investigate, by electron microscopy, the subcellular localization of bFGF-like immunoreactivity in neuroglias and neurons of the trigeminal motor and mesencephalic nuclei. Immunostaining intensity differed from astrocyte to astrocyte; in astrocytes labeled heavily with bFGF antiserum, the precise subcellular location of immunoreaction deposits was difficult to determine, whereas mildly labeled astrocytes contained reaction products in subcellular regions apart from gliofilaments, mitochondria and Golgi apparatus. A comparison of immunostained sections with negative control ones at the light and electron microscopic levels revealed that astrocyte nuclei occasionally showed bFGF immunoreactivity. Immunoreactive astrocyte processes were also found in close apposition to blood vessels. bFGF was detected mainly in intracellular structures close to free ribosomes and the endoplasmic reticulum of immunoreactive oligodendrocytes and neurons; microglias rarely showed immunostaining. The nuclei of the cells with bFGF contained immunoreaction deposits of varying intensity, mainly in the euchromatin and rarely in the heterochromatin. Occasionally, bFGF of neuroglial origin accumlated in the vicinity of the interface between neurons and neuroglias. The extracellular matrix was not immunoreactive in any of the areas examined. These findings suggest that certain populations of astrocytes and oligodendrocytes as well as neurons in the normal brain contain bFGF-like substances.View full abstractDownload PDF (5270K) -
Kazuyuki SHIMADA, Iwao SATO, Gen MURAKAMI, Toshinori IKEDA1993 Volume 69 Issue 6 Pages 345-351
Published: March 22, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSTeeth from Megalobatrachus japonicus were prepared for scanning electron microscopy (SEM) and electron microprobe analyzer (EMPA) to assay the elements of the enamel layer. The enamel layer of each of tooth is thin and the arrangement of the enamel crystals is regular in the Megalobatrachus japonicus. Based on calcium, phosphate and magnesium levels of distribution, the calcification level of the enamel layer differs between the labial and lingual surfac es. Concentrations of iron and fluoride are located in both portions of the enamel layer as well. The presence of trace elements and distribution of calcified levels are related to the mineral formation in the enamel layer and function of the teeth during feeding.View full abstractDownload PDF (2217K) -
Takashi NAKANO1993 Volume 69 Issue 6 Pages 353-359
Published: March 22, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe NaOH cell-maceration method was applied to the mouse colorectal mucosa to demonstrate the threedimensional architecture of the lamina propria, especially that of the glandular crypts. In the surface of the lamina propria, there existed the longitudinal ridges and furrows. In the ridges, the crypts opened to the lumen individually, and had no internal partitions separating a crypt. The lateral walls of the crypts in the ridges had numerous hemispherical concavities into which the epithelial cells were tightly fitted. In the furrows, on the other hand, the crypts were subdivided into several smaller crypts by the internal partitions, and were termed as the "protocrypts". Considered from these findings, the crypts in the ridges appear to be matured, while the protocrypts in the furrows to the crypts undergoing process of proliferation. Further, it is suggested that the crypts proliferate in the furrows and, then, populate in the ridges as the mature crypts.View full abstractDownload PDF (2394K) -
Masahiro KOIZUMI, Masaharu HORIGUCHI, Shin'ichi SEKIYA, Sumio ISOGAI, ...1993 Volume 69 Issue 6 Pages 361-367
Published: March 22, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSAn anomalous nerve supply from the hypoglossal nerve (XII) to the sternocleidomastoid muscle (SM) was observed in the right neck of an 82-year-old Japanese female. This nerve branch arose from the hypoglossal nerve at the origin of the superior root of the ansa cervicalis. The nerve fiber analysis revealed that this branch consisted of fibers from the hypoglossal nerve, the first and the second cervical nerves and had the same component as the superior root of the ansa cervicalis. SM appeared quite normal and the most part was innervated by the accessory nerve and a branch from the cervical plexus. The anomalous branch from XII supplied the small deep area near the anterior margin of the middle of the sternomastoid portion of SM. It is reasonable to think that the small deep area of SM, which was innervated by the anomalous branch from XII, occurs as the result of fusion of the muscular component from infrahyoid muscles. If the muscular component does not fuse with SM, it is thought to appear as an aberrant muscle such as the superior sternoclavicular muscle (Hyrtl) which is also supplied from a branch of the superior root of the ansa cervicalis.View full abstractDownload PDF (1987K) -
Keisuke YAMASHITA, Chairuddin RASJAD, Takashi KOBAYASHI, Mineo YASUDA1993 Volume 69 Issue 6 Pages 369-383
Published: March 22, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSA fine structural study was undertaken on the area called the anterior necrotic zone of leg buds of the chick embryo at stages 22-29. Physiologically dying cells, which were distributed in the mesenchymal area, were characterized by increased electron density of the cytoplasm, appearance of electron lucent vacuoles and condensed chromatin pattern. Many of the dying cells seemed to be fragmented into several pieces of cell debris during the necrotic process. Two kinds of cells were observed to take up the necrotic cells; one type, large cells 15μm or more in size and irregular in shape, which had lysosomes, extended microvillus-like cytoplasmic projections and pseudopods, took up pieces of necrotic cells by phagocytosis and stored them in acid phosphate-negative and -positive vacuoles. The other type was several μm in diameter with numerous free ribosomes and stored a small number of vacuoles containing cell debris. At stage 29, profiles of necrotic cells lying free in the mesenchymal area, and cells containing one or two vacuoles filled with cell debris decreased in number as well as large cells containing numerous vacuoles, whose contents seemed to be degraded. When pieces of the anterior portion of chick leg buds at stage 24 were organ-cultured for 3 days after glutaraldehyde-fixed mouse red blood cells were injected, the red blood cells were taken up into cells in the mesenchymal area. Formation of large cells containing numerous vacuoles stuffed with red blood cells was rare. These findings may imply that most necrotic cells in the anterior necrotic zone are disposed of by macrophages and that some are endocytosed by mesenchymal cells.View full abstractDownload PDF (5664K) -
Akiko OKUMURA, Yasutomo IWAI-LIAO1993 Volume 69 Issue 6 Pages 385-399
Published: March 22, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe genesis, development and growth of the mouse stapediovestibular joint (SVJ), which contains the annular ligament, and the temporomandibular joint (TMJ) were examined in an attempt to study the stress-bearing articular tissue that is thought to be derived from embryonic mesenchyme; the findings were also compared with those in the ossicular joints. The following conclusions were obtained: 1) The articular cartilage of the mandibular condylar process, stapedial foot plate and otic capsule is derived from fetal fibrous articular tissue.2) The fetal TMJ developed into a typical double diarthroses containing an articular disc.3) The fetal syndesmodial SVJ differentiated into the annular ligament containing characteristic palisade-like fibroblasts and hammock-like fibers; no interzone formation or synovial cavities were observed in the completed and mature syndesmodial SVJ.4) Like the TMJ, the major elastic system fibers in the annular ligament were also mechanical-resistant elaunin.5) Elastogenesis was closely related to functional and mechanical factors in the auditory ossicular chain, TMJ and annular ligament; the mature incudomalleal and incudostapedial joints contained mainly mature elastic fibers, but the mature SVJ and immature TMJ contained mainly pre-elastic elaunin fibers.6) Stress elastosis, turnover of the fibrillar component and age changes in cellular and fibrous components were not evident in either the mature SVJ or the young functional TMJ.View full abstractDownload PDF (6237K)
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