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Volume 65 (1988 - 198･･･
- Issue 6 Pages 353-
- Issue 5 Pages 245-
- Issue 4 Pages 171-
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Volume 65, Issue 6
Displaying 1-6 of 6 articles from this issue
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Kiminao MIZUKAWA, Shigeto KANDA, Ryoichi TOBITA, Nagayasu OTSUKA1989 Volume 65 Issue 6 Pages 353-367
Published: 1989
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe release mechanism of the rat atrial granules containing atrial natriuretic polypeptide (ANP) was examined. In addition, the ultrastructure of right atrial myocardiocytes incubated in a medium containing an excess calcium and calcium ionophores (A 23187) was investigated by electron microscopy. After 3-15 minute incubation in the medium, many ANP-containing atrial specific granules were detected in the vicinity of the subsarcolemmal region, and invagination of the plasma membranes occurred as well as a large number of vacuoles were observed. After incubation with an excess calcium and calcium ionophores for 40 minutes, the atrial specific granules had completely disappeared and numerous vacuoles appeared in the sarcoplasm of the atrial myocardiocytes. Thus, release of ANP and their reabsorption by membranes were accelerated by the presence of calcium and calcium ionophores.View full abstractDownload PDF (7124K) -
Bunsuke OSOGOE, Masako UMEBAYASHI-YANAGI1989 Volume 65 Issue 6 Pages 369-379
Published: 1989
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSConnective tissue cells, Macrophage precursors, Rat, [14C]adenine, Autoradiography Summary: After 3 daily injections of [14C]adenine([14C]A)(1μCi/g body weight each)into young adult rats, the large majority of typical, spindle-shaped fibroblasts in the connective tissues of most organs showed almost no labeling, with the exception of a few weakly labeled ones. In contrast, in the interstitial connective tissues of pancreas and heart, unexpectedly numerous mesenchymal cells were found to exhibit especially strong labeling with [14C]A even after RNase treatment. Such cells had a large, round or ovoid, euchromatic nucleus and showed the blast-like appearance. The term mesenchymal cells was used to denote a special type of connective tissue cells that retain the potentiality of embryonic mesenchymal cells. Double labeling experiments using [14C]A and trypan blue by 7 daily injections(1μCi/g body weight of [14C]A and 3 ml/rat of 0.5% aquous solution of trypan blue each) disclosed that the mesenchymal cells exhibiting heavy [14C]A labeling did not ingest trypan blue at all. It was further revealed that, in addition to the mesenchymal cells, macrophages showing a remarkable trypan blue uptake were also labeled with [14C]A, though to a lesser extent than were the former cells. Moreover, transitional forms between the mesenchymal cells and macrophages were occasionally observed. The transitional forms were characterized by having the capacities not only for a fairly high rate of DNA synthesis but also of ingesting trypan blue, though to a slight extent. On the basis of the above findings, it can be stated that the mesenchymal cells showing heavy labeling with [14C]A are most likely the precursor cells of macrophages.View full abstractDownload PDF (5335K) -
Takashi SUZUKI1989 Volume 65 Issue 6 Pages 381-389
Published: 1989
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe lamination of the masseter muscle in 21 Japanese serows of different sexes and ages was studied by the method of Yoshikawa et al. who proposed a lamination theory for this muscle. The masseter muscle in the Japanese serow was found to be composed of I) the proper masseter muscle which included 1) the first superficial, 2) the second superficial, 3) the intermediate and 4) the deep masseter muscles, in which the deep masseter muscle could be subdivided into a pars anterior and pars posterior and II) the improper masseter muscle which included 5) the maxillomandibular and 6) the zygomaticomandibular muscles, in which the maxillomandibular muscle was further divided into first and second layers. These findings indicate that the lamination of the masseter muscle in the Japanese serow is the same as that in the goat and sheep.View full abstractDownload PDF (914K) -
Seiji KATO, Ryosuke MIYAUCHI1989 Volume 65 Issue 6 Pages 391-403
Published: 1989
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe distinction between lymphatic capillaries and blood capillaries in the mouse tongue was studied enzyme-histochemically by light and electron microscopy. The lymphatic walls are characterized by a strong 5'-nucleotidase (5'-Nase) activity, whereas those of the blood capillaries reveal a significantly lower or no activity. The alkaline phosphatase (ALPase) activity, on the other hand, is markedly higher in the blood capillaries than in the lymphatic capillaries. The specific reaction of 5'-Nase activity in the lymphatic capillaries is obtained by simultaneous inhibition of ALPase on incubation in a medium (Wachstein and Meisel,1957) with L-tetramisole for 5'-Nase histochemistry. The distribution and intensity of the 5'-Nase activity in the lymphatic capillaries can be adequately visualized by comparison with serial cryostat sections for histochemical detection under light and backscattered imaging scanning electron microscopes. The reaction products of the 5'-Nase activity are localized on the outer surface of the cell membrane of the lymphatic endothelial cells, whereas those in the blood capillaries reveal a weak or no reaction. The present results demonstrates satisfactory isolated visualizations of 5'-Nase activity in the lymphatic capillaries and of ALPase activity in the blood capillaries.View full abstractDownload PDF (8147K) -
Masatake IMAI, Taizo SHIBATA, Keiichi MORIGUCHI, Masashi KINBARA1989 Volume 65 Issue 6 Pages 405-411
Published: 1989
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSAccording to the P. N. A., the N. A. J. and some scholars, the sublingual gland has the ductus sublingualis major and ductus sublinguales minores. This means that the gland is a polystomatic gland. We intended to determine whether the so-called polystomatic gland exists or not. 1. According to the P. N. A., the N. A. J. and some scholars, the gl. sublingualis has the ductus sublingualis major and ductus sublinguales minores. This means the gland is a polystomatic gland. However, the formation of one gland with plural excretory ducts is embryologically impossible, in other words, the polystomatic gland does not exist. 2. Many scholars described that the gl. sublingualis was composed of the gl. sublingualis major and gll. sublinguales minores. However, they are completely different kinds of glands. Accordingly, we suggest the terms for these glands: the gl. sublingualis and its ductus sublingualis ("major"is useless), the gll. sublinguales minores and their ductus sublinguales minores. 3. The N. A. V. J. and some scholars use the term gl. sublingualis polystomatica or parvicanalaris. However, this is a group of a number of independent glands each of which has its own excretory duct. Such a gland should not be regarded as a single gland. We suggest that the term gll. sublinguales minores and their excretory ducts should be replaced with the term the ductus sublinguales minores. 4. The gl. lingualis anterior, gl. retromolaris and gl. lacrimalis are not single glands but a group of several independent glands each of which has its own excretory duct. Accordingly, they should be termed the gll. linguales anteriores, gll. retromolares and gll. lacrimales such as the gll. labiales, gll. buccales and gll. palatinae.View full abstractDownload PDF (1890K) -
Shigeru KOBAYASHI, Shusei ARAI, Soichiro TOMO, Taeko SHIMODA, Akitatsu ...1989 Volume 65 Issue 6 Pages 413-427
Published: 1989
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe tongue and lingual papillae of the Japanese Insectivora, the Shinto shrew (Sorex caecuiens saevus), the long-clawed shrew (S. unguiculatus), the dsinezumi shrew (Crocidura dsinezumi dsinezumi) and the Japanese water shrew (Chimarrogale himalyica platycephala), were observed by scanning electron microscope. The tongue of these animals had two vallate papillae. In two species of the Sorex a papilla in the vallate papilla was surrounded by two separated trenches, but in the other species it was surrounded by only a continuous trench and a clear vallum. The fungiform papillae in the Sorex were less developed than those of the other species. In the Sorex and Crocidura, there was no filiform papilla on the lingual apex. These genera, however, have papillary projections in the margin of the lingual apex. The results of this investigation suggest that the Sorex and Crocidura indicate an ancient form of the mammalian tongue. These characters, furthermore, were compared among seven species in six genera added three species observed by Kobayashi et al. (1983)to this study.View full abstractDownload PDF (4217K)
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