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Volume 88 (2011)
- Issue 4 Pages 133-
- Issue 3 Pages 89-
- Issue 2 Pages 43-
- Issue 1 Pages 1-
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Volume 88, Issue 4
Displaying 1-4 of 4 articles from this issue
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Yasutomo IWAI, Yoshifumi MATSUDA, Michiko NAKATSUKA, Yutaka MIKAMI, Sh ...2012 Volume 88 Issue 4 Pages 133-140
Published: February 20, 2012
Released on J-STAGE: May 23, 2012
JOURNAL FREE ACCESSHMS0014 cells were GBR-engineered to proliferate and differentiate into mature osteoblast(Ob)-like cells, which initiated hard tissue matrix deposition in both monolayer and PuraMatrix 3-D cultures. Subsequently, the osteogenesis initiated with attachment/adhesion of HMS0014 cells on either Titanium (Ti) or Ti alloy discs modified with osteoconductive/ osteoinductive surface textures/substrates (e.g., Disc-AO, Disc-HA, Disc-SPI) was histologically assessed. The results obtained were as follows: 1) The HMS0014 cells actively proliferated and differentiated into mature Obs to initiate mineralisation of the ECM since day 1 in both monolayer and 3-D cultures; mineralization was prominently progressed between day 7 and day 14 of cultures. 2) The SEM of 60-minute(min)s specimens demonstrated a loose distribution of proliferating spherical-to-polygonal (10 to 40 μm in diameter, avg.) cells with a bulging cell body sending out many minute filopodia and some lamellipodia to attach with the substrate in the concavities. 3) In the 180-min specimens, the cultured HMS0014 cells actively proliferated and spread into flat, large polygonal cells with prominent lamellipodia and dendritic filopodia (30 μm × 90 μm to 100 μm × 200 μm, approx.) to employ cell-to-substrate and intercellular attachments. 4) On the other hand, the present immunohistochemistry of the attached HMS0014 cells demonstrated the co-expression of F-actin (actin filaments of the cytoskeleton) and CD51 (αV integrin) in both the 60-min and 180-min specimens. We concluded that the present GBR method enhanced HMS0014 cells to initiate an osteogenesis process with a direct bone-to-substratum contact on Ti discs which were subject to different surface modifications.View full abstractDownload PDF (3153K) -
Shota TAJIRI, Tatsuya FUKUI, akira SAWAGUCHI, kazuya YOSHINAGA2012 Volume 88 Issue 4 Pages 141-146
Published: February 20, 2012
Released on J-STAGE: May 23, 2012
JOURNAL FREE ACCESSTo understand the cytochemical properties of epididymal epithelial cells, the characteristics of glycoconjugates in the mouse epididymis were examined using the technique of lectin histochemistry combined with immunohistochemistry. Characteristic staining patterns depending on the type of lectins were observed in the epididymal epithelium. Principal cells expressed N-acetyl-D-galactosamine (GalNAc), N-acetyl-D-glucosamine (GlcNAc), and Fucose in the proximal region of the epididymis and Mannose, glucose, and galactose in the distal region of the epididymis. Basal cells expressed Mannose, glucose, galactose, and GlcNAc in the proximal region and galactose in the distal region. on the other hand, clear cells expressed various sugar residues and differences among regions were not observed. Interestingly, principal cells, clear cells, and basal cells specifically reacted with Ulex Europaeus-Agglutinin I (UEA-I lectin), Maackia Amurensis-Lectin I (MAL-I lectin), and Griffonia simplicifolia Lectin I-B4 (GS-I B lectin), respectively. These findings indicate that the selectivity in lectin reactivity for distinct cell types and segment-dependent staining in the epididymis may be related to cellular and regional differences in function. Furthermore, because some lectins stain particular cells or cellular compartments selectively, these lectins could be useful markers for histopathological evaluation of diseases or diagnosis of male infertility.View full abstractDownload PDF (1708K) -
Huayue CHEN, Shoichi EMURA, Sachio NAGASAKI, Kin-ya KUBO2012 Volume 88 Issue 4 Pages 147-151
Published: February 20, 2012
Released on J-STAGE: May 23, 2012
JOURNAL FREE ACCESSThe duplication of the inferior vena cava (IVC) is a rare, but well-recognized anomaly. Duplicated IVC has a significant relevance for retroperitoneal surgery and venous interventional radiology. We report a case of duplicated IVC, which was observed during routine dissection of an 84-year-old Japanese female cadaver. The interiliac vein between the duplicated IVC ran obliquely upwards from left to right. We performed systematic literature review of published reports based on Pubmed and Medline from 1967 to 2011. Of 109 cases with IVC anomalies identified by the literature search, 22 cases (20.2%) displayed no interiliac anastomosis. The interiliac vein connecting duplicated IVC existed in 74 cases (67.9%). According to the running direction of the interiliac vein, we found that the vein ran from left to right in 42 cases, coursed from right to left in 19 cases, and ran horizontally in 13 cases. Thirteen left IVC displayed symmetrical-to-normal connection with the bilateral common iliac veins. Awareness of these venous variations is necessary to reduce surgical risk and to determine strategy in interventional radiology.View full abstractDownload PDF (650K) -
Shota TAJIRI, Tatsuya FUKUI, Akira SAWAGUCHI, Kazuya YOSHINAGA2012 Volume 88 Issue 4 Pages 153-157
Published: February 20, 2012
Released on J-STAGE: May 23, 2012
JOURNAL FREE ACCESSUsing lectin histochemistry combined with immunohistochemistry, we recently demonstrated that the principal, clear, and basal cells in the adult mouse epididymis specifically react with UEA-I, MAL-I, and GS-IB lectins, respectively. The present study examined the distribution of the lectin-binding sites for some lectins on the epididymal epithelium during postnatal development. galactose staining with GS-IB was first detected in some of the undifferentiated epithelial cells in mice aged 1 week, and this characteristic became specific to basal cells in mice aged 2 weeks and above. Fucose staining with UEA-I was first detected in the principal cells in mice aged 3 weeks. Staining of sialic acid with MAL-I was first detected in all undifferentiated epithelial cells in mice aged 1 week, and this characteristic became specific to the narrow and clear cells in all regions and to the principal cells in regions IV and V in mice aged 3 weeks. The results indicate that epididymal differentiation is characterized by the expression of cell-and region-specific sugar chains that appear early during postnatal development before the sperm arrives in the epididymis.View full abstractDownload PDF (1539K)
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