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Volume 90 (2013)
- Issue 4 Pages 79-
- Issue 3 Pages 53-
- Issue 2 Pages 23-
- Issue 1 Pages 1-
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Volume 90, Issue 4
Displaying 1-6 of 6 articles from this issue
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CONTENTS
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Shoichi EMURA, Toshihiko OKUMURA, Huayue CHEN2014 Volume 90 Issue 4 Pages 79-83
Published: 2014
Released on J-STAGE: May 09, 2014
JOURNAL FREE ACCESSWe examined the dorsal lingual surface of an adult fishing cat (Prionailurus viverrinus) by scanning electron microscopy. The filiform papillae on the lingual apex had several pointed processes. The connective tissue core of the filiform papillae resembleda a well in shape. The filiform papillae on the anterior part of the lingual body were large and cylindrical in shape. The connective tissue core of the filiform papillae consisted of a large conical papilla. The filiform papillae on the central part of the lingual body were large and conical. The connective tissue core of the filiform papillae consisted of a large main process and some secondary processes. The connective tissue core of the fungiform papillae did not have processes. The vallate papillae were surrounded by a groove and a pad. The top of the connective tissue core of the vallate papillae had a rough surface with no spines.View full abstractDownload PDF (2134K) -
Shoichi EMURA, Toshihiko OKUMURA, Huayue CHEN2014 Volume 90 Issue 4 Pages 85-88
Published: 2014
Released on J-STAGE: May 09, 2014
JOURNAL FREE ACCESSWe examined the dorsal lingual surface of an adult brush-tailed rat kangaroo (Bettongia penicillata) by scanning electron microscopy. The filiform and fungiform papillae on the lingual apex and body consisted of a main papilla and secondary papillae. The connective tissue core of the filiform papillae on the lingual apex was cylindrical in shape with a crushed top. The connective tissue core of the filiform papillae on the lingual body had one large and several small processes. The fungiform papillae were round in shape. The connective tissue core of the fungiform papillae had several depressions on its top. The surface of the vallate papillae was rough and the papillae were surrounded by a groove and a pad. Several long conical papillae derived from the posterolateral margin of the tongue where foliate papillae have been shown to be distributed in many other animal species. The long conical papillae were very similar to those of the koala and opossum.View full abstractDownload PDF (2076K) -
Shin-ichi ABE, Mitsuhiro AOKI, Tadashi NAKAO, Masaaki KASAHARA, Jose F ...2014 Volume 90 Issue 4 Pages 89-95
Published: 2014
Released on J-STAGE: May 09, 2014
JOURNAL FREE ACCESSWe examined the topohistology of the subscapularis tendon at the glenohumeral joint in 10 mid-term (15-16 weeks of gestation) and 10 late-stage (27-32 weeks) human fetuses. At both stages, there were two patterns of terminal course of the subscapularis tendon: 1) the tendon was tightly attached to the medial part of the joint capsule and extended anterosuperiorly along the capsule to the lesser tubercle (7/10 mid-term fetuses; 5/10 late-stage fetuses); 2) the tendon passed superiorly through the joint cavity for a long distance in combination with the subcoracoid bursa opening widely to the joint cavity (3/10 mid-term fetuses; 5/10 late-stage fetuses). The lower glenoid labrum tended to be well developed in the former pattern because the subscapularis tendon did not interfere with the superior extension of the labrum. With only one exception (late stage), the capsule-attaching tendon was seen in fetuses in which the coracoid process was located on the superior side of the lesser tubercle, whereas the intra-articular tendon accompanied the coracoid process at the same supero-inferior level of the tubercle. Thus, the topographical relationship between the coracoid process and lesser tubercle in fetuses seemed to determine the courses of the subscapularis tendon at the glenohumeral joint. The present variation in the subscapularis tendon was likely connected with the adult morphologies of the middle and inferior glenohumeral ligaments or folds, whose variations are well known.View full abstractDownload PDF (5665K) -
Keiichi MORIGUCHI, Norikazu OHNO2014 Volume 90 Issue 4 Pages 97-100
Published: 2014
Released on J-STAGE: May 09, 2014
JOURNAL FREE ACCESSPolymorphonuclear leukocytes (PMNs) engaged in phagocytosis produce reactive oxygen species (ROS), such as those that occur in an activated NADPH oxidase reaction, to eliminate ingested microorganisms. The translocation of NADPH oxidase components to produce antimicrobial free radicals from the vesicles to the phagosomes may be important. Hydrogen peroxide (H2O2) derived from O2- has been observed by electron microscopy using a cerium method. However, 2’-7’-dichlorofluorescin diacetate can also detect H2O2 through fluorescence. The main objective of the present study was to measure the H2O2-dependent fluorescence of PMNs after opsonized zymosan A (OPZ) phagocytosis using a microplate reader under different fixation conditions, including 0.5, 1, and 10% glutaraldehyde (GA) individually for 1, 5, 10, or 30 min. An additional objective was to visualize, through the use of electron microscopic cytochemistry, the process of H2O2 generation in OPZ phagocytic fixed PMNs. The fixed PMNs showed that the largest fluorescent value was produced by a concentration of 0.5% GA for all fixation times. This suggested that the fixation of PMNs with a high concentration of GA inhibited phagocytosis and produced ROS. In the fixed PMNs, electron microscopic results showed that after 1 min of mixing, some PMNs attached to particles and exhibited mild deposits in their secretory vesicles. When PMNs engulfed particles, free radical-producing vesicles had enhanced reaction deposits 10 min later and fused to the phagosomal membrane, releasing numerous free radicals into the lumen. Time-dependent H2O2 production was enhanced in the secretory vesicles, some of which were fused exactly to the phagosome membranes.View full abstractDownload PDF (1234K) -
Masataka SUNOHARA, Shigeru MORIKAWA, Akira FUSE, Iwao SATO2014 Volume 90 Issue 4 Pages 101-106
Published: 2014
Released on J-STAGE: May 09, 2014
JOURNAL FREE ACCESSThrombopoietin (TPO) and its receptor, c-Mpl, play the crucial role during megakaryocytopoiesis. Previously, we have shown that the promoter activity of c-mpl induced by TPO is modulated by transcription through a PKC-dependent pathway and that GATA(-77) is involved as a positive regulatory element in TPO-induced c-mpl gene expression in the megakaryoblastic CMK cells. In this research, to examine participating possibility of GATA promoter element in TPO- induced c-mpl gene expression through a PKC-independent pathway, the promoter activity of site-directed mutagenesis and the effect of potein kinase C modulator were measured by a transient transfection assay system. Together with our previous results on the TPO-induced c-mpl promoter, this study indicates destruction of -77GATA in c-mpl promoter decreased the activity by 47.3% under existence of GF109203. These results suggest that GATA promoter element plays significant role in TPO-induced c-mpl gene expression through a PKC-independent pathway.View full abstractDownload PDF (1027K)
CORRIGENDUM
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2014 Volume 90 Issue 4 Pages 107
Published: 2014
Released on J-STAGE: May 09, 2014
JOURNAL FREE ACCESSDue to author’s request, one of the author’s name (Toshihito YOSHIHITO) should be corrected to Toshihito YOSHIMOTO. The corrected author’s name list is as follows:
Shinichi ABE1, Tadashi NAKAO1, Toshihito YOSHIMOTO1, Seppo PARKKILA2,
Gen MURAKAMI3 and Baik Hwan CHO4View full abstractDownload PDF (480K)
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