It was revealed by the pr e sent study that neutrophils and eosinophils were demonstrable in the colostrum, that the colostrum bodies were entirely peroxidase negative, and that neutrophils arising from the parotid gland were identified by the methods of the specially-made suction cup as well as the tissue sections; absolute counts of leucocytes in 1.0 ml. parotid saliva of 20 normal human subjects ranged from 0 to 794. Furthermore, evidence was presented that the peroxidase stain is definitely preferable to the Romanowsky mixture for differential staining of the myeloid leucocytes in the degenerating stages.

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In order to obtain a better understanding of the essential nature of the cellular component in the saliva, reactions of glycogen, iodine, peroxidase, Dopa oxidase and of iron as well as staining of the plasma membrane were tested on the saliva smears derived from 114 human subjects aged from 6 to 56 years. It was made clear through the present study that the glycogen-, peroxidase-, Dopa oxidase-reactions of the. salivary corpuscles generally became weaker with increase of the degeneration in the saliva, and that, to our surprise, a very small fraction of the salivary corpuscles indicated a more intense glyc ogen reaction than normal neutrophil leucocytes in the peripheral blood, furthermore that the nuclei of the salivary corpuscles were occasio nally Dopa and iodine reactions positive, which was never seen in the leucocytes of the peripheral blood. Most of the salivary corpuscles are thought to be the neutrophil leucocytes which stain in considerably different degrees from those of the peripheral blood. The peroxidase and Dopa reactions of the oral epithelial cells were negative, while glycogen, and iodine reactions of them were positive, in addition to this, the plasma membrane of them was stained very distinctly as compared with that of the salivary corpuscles.

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1) We observed successive l y the changes on alkaline phosphatase, acid phosphatase and PAS positive substances of the autonomic nerve cells of the myenteric and submucous plexus of the small intestine of mouse caused by the pilocarpin injection.
2) In the myenteric plexus o f the control case, we classified each three types of the nerve cells according to the intensity of the activity of alkaline phosphatase and acid phosphatase and to the stainability of the PAS positive substances.
3) Alkaline p h osphatase In the myente r i c plexus, all kinds of cells increase their activity on 15 and 30 minutes cases after injection. On one hour case, the activity decreases and is almost the same or somewhat weaker than the control case. 'O n two, four and six hours cases, the activity is the same as the control case. The changes on the cell of the first type is more feeble than that of the other two. The cells of the sub m ucous plexus show the similar change to those of the myenteric plexus. But the degree of the activity on one and two hours cases is less than the myenteric plexus and four hours case shows the same activity as the control case.
4) Acid phosphatase In the my e nteric plexus, the activity increases on the 15 minutes case a little but further increases on the 30 minutes and one hour cases. But the activity decreases on the two hours case, to the degree which is the same as or somewhat weaker than the control case. On four and six hours cases, the activity is the same as the control case. The changes on the cell of the first type are not so significant as those of the other two. In the submucous plexus, the nerve cells show the same changes as those of the myenteric plexus did. But in some cells of the submucous plexus, the activity does not decrease distinctly on two, four and six hours cases and is kept to the same degree as one hour case. Cytochemical studies of the temporary changes etc.
5) PAS positive substance, In the cells of both plexuses, the stainability increases considerably on the 30 minutes case. On one, two, four, and six hours- cases,. the stainability is the same as on 30 minutes case. The PAS positive substances are not glycogen but something else.

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