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Volume 81 (2004)
- Issue 6 Pages 119-
- Issue 5 Pages 85-
- Issue 4 Pages 57-
- Issue 2.3 Pages 33-
- Issue 1 Pages 1-
Predecessor
Volume 81, Issue 4
Displaying 1-4 of 4 articles from this issue
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Kiyokazu FUKUI, Rieko KOMINAMI, Ayumi KANEUJI, Tadami MATSUMOTO, Harum ...2004 Volume 81 Issue 4 Pages 57-62
Published: 2004
Released on J-STAGE: January 18, 2008
JOURNAL FREE ACCESSWe report a new method of perfusion fixation for the proximal one-third of the femur of the Japanese white rabbit. Fluids to flush the blood and fix the marrow were injected into the abdominal aorta and drained from the stump of the femur. The oozing of the fluids from the stumps guaranteed complete flushing and fixation. The new method facilitated fixation and decreased the volume of necessary fluids.
Scanning electron microscopy (SEM) images of bone marrow fixed using the new method and using the conventional method did not differ. Large fat globules were not observed in the SEM specimens produced using either the new or the conventional method.View full abstractDownload PDF (319K) -
Akemi KAMIYAMA, Jun GOTO, Noboru GOTO, Kazumi TSURUKIRI, Yoshiaki HOSA ...2004 Volume 81 Issue 4 Pages 63-66
Published: 2004
Released on J-STAGE: January 18, 2008
JOURNAL FREE ACCESSIn order to study the precise structures of the human eyelids, we made serial sections, and found a plug-in structure protruding from the lacrimal caruncle and reaching into the lacrimal point. We will show a photo of this plugin structure, as we believe it plays an important role in the lacrimal drainage system.View full abstractDownload PDF (103K) -
Iwao SATO, Kiyoshi KONISHI2004 Volume 81 Issue 4 Pages 67-74
Published: 2004
Released on J-STAGE: January 18, 2008
JOURNAL FREE ACCESSThere is not fine information regarding the influence of diet on development of masseter muscle and its mitochondria. Objective of this study is to compare mitochondrial enzyme activity, and morphology of masseter muscle fiber cells and its mitochondria prepared from rats fed soft diet to those fed hard diet. Cross-sectional area of fiber cells and number of mitochondria per unit area prepared from rats fed hard diet were greater than those of animals fed soft diet on postnatal day 60, and these differences decreased under both feeding conditions on days 120. Structure of mitochondrial cristae of rat fed hard diet was clear but that of fed soft diet ambiguous and contains many halos. Mitochondrial succinate-O2 and NADH-O2 oxidoreductase activities isolated from rats fed hard diet were higher than those of soft diet group over the period from days 30, and differences in both diet groups became the largest on days 60, and decreased on days 120. Our results indicated that development of masseter muscle fiber cells and mitochondria is hindered when rats are fed soft diet, but recover partially later, and hard diet is required for normal development of masseter muscle.View full abstractDownload PDF (339K) -
Akio HIURA, Hiroshi NAKAGAWA2004 Volume 81 Issue 4 Pages 75-84
Published: 2004
Released on J-STAGE: January 18, 2008
JOURNAL FREE ACCESSLittle is known whether capsaicin affects on the nonspecific acetylcholinesterase (NsAchE) positive nerves in the cornea. To examine this point, the density of the corneal nerve fibers stained with NsAchE method was compared with that of control. Capsaicin (50 mg/kg) was injected once into the dorsal skin of rats at 2 day after birth. As controls, a vehicle was similarly injected into animals of the same age. At 5 (n=3), 17 (n=4), 45 (n=4) and 75 (n=3) days after treatment, the bilateral corneas were excised from capsaicin treated (n=14) and age-matched control animals (n=14). The entire subepithelial corneal nerves stained in whole mount preparations were hand traced. Their density was represented as mm/mm2 by converting the total areas of the nerve fibers into the total length of them using computer image analysis system. No significant differences in the nerve fiber density were seen in the control and capsaicintreated corneas at the survival times examined. Even high doses of neonatal capsaicin injection (total: 150 mg/kg) no NsAchE-reacted corneal nerves showed a significant decrease. However, the time of wiping by instillation of 100μM capsaicin onto the corneas severely reduced (analgesia to chemical irritant) in the neonatal capsaicin-treated rats (50 mg/kg, n=13) compared with those of controls (n=13) at 17, 45 and 75 day survivals. This was closely associated with the decrease of PGP-ir intraepithelial nerve fibers. The corneal nerves reacted with the NsAchE seemed to be capsaicin resistant and have a functional property except the chemical nociception.View full abstractDownload PDF (449K)
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