The present paper deals with the ultrastructure of the amelogenesis of the upper major incisor of the rabbit. Ultrastructural changes in the enamel organ of the rabbit incisor, compared to those of the rat incisor, are described on the basis of a classification into six zones/stages, ranging from the growing end to the junctional epithelium, as follows: proliferation, differentiation, secretion, early maturation, late maturation and regression.
The inner enamel epithelial cells of the proliferation stag e had wide intercellular spaces with the mitochondria located distally towards the dental papillary cell. The metabolic pathway was therefore considered to be established through this distal site. During the differentiation and secretion stages, the nucleus and mitochondria were located on the basal side (towards the stratum intermedium), but the r-ER and Golgi apparatus were on the distal side. The intercellular spaces became narrower and terminal webs were found on both the basal and distal sides. Numerous secretory granules were formed in the well-developed Golgi apparatus. The Tomes' process disappeared in the early maturation stage, and the enamel matrix began to be absorbed. In the late maturation stage, respective groups of ruffle-ended and smooth-ended ameloblasts were found one after the other three or four times. Intracellularly, a network arrangement of tonofibrils was observed in the regression stage. The cells of the stratum intermedium in both the differentiation and secretion stages prolonged their cellular processes, being interconnected by many desmosomes, and surrounded the basal cone-like projection of the ameloblasts. In the early and late maturation stages, flat-arranged papillary cells in contrast to those of the rat, contacted the blood capillaries. In the regression stage, the tonofibrils ran along the cell axis and the half desmosomes paralleled the basement membrane facing the surrounding connective tissue. The stellate reticulum began to decrease suddenly in the differentiation stage and finally disappeared in the middle of the secretion stage.
The outer enamel epithelial cells were transformed into a cylindrical, cubical and flattened shape in the proliferation stage, and arranged in a wavy course, holding fenestrated blood capillaries in the differentiation and secretion stages. The enamel formation of the rabbit incisor ended within a short period, in accordance with a shorter, early maturation and longer, late maturation, as well as other characteristic features of the enamel organ.
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