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Volume 70 (1993 - 199･･･
- Issue 6 Pages 247-
- Issue 5 Pages 195-
- Issue 4 Pages 139-
- Issue 2-3 Pages 41-
- Issue 1 Pages 1-
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Volume 70, Issue 4
Displaying 1-6 of 6 articles from this issue
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Setsuko NODA, Tomoko INOUE1993 Volume 70 Issue 4 Pages 139-149
Published: October 20, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSWe examined the immunohistochemical localization of protein kinase C (PKC) in chick embryonic and chick duodenal villi contained in cultured duodena using monoclonal antibodies that recognize types I,2 and 3 at the light and electron microscopic levels. In the light microscopic studies, staining with the type 2 and 3 PKC antibodies revealed immunoreactivities in the absorptive epithelial cells of the villi that approximated the time course after hatching, but was weak and partial just before the time of hatching. The epithelial cells of crypts seemed to lose their stain at all times. In the transmission electron microscopic studies, positive immunoreaction was found predominantly in the endoplasmic reticulum and nucleus, and also in the terminal web in the absorptive epithelial cells of villi. In the cultured duodenal fragments, a change in the distribution of PKC was observed on the sheet-like duodenal fragments and its immunoreaction was mainly seen on the basal side of absorptive cells.
In contrast, no change in the staining of PKC was revealed on the ring-like duodenal fragments.
These results suggested that PKC in the chick duodenal villi may be functionally related with the differentiation of absorptive epithelial cells and sensitive to changes in the cellular interactions of absorptive epithelial cells.View full abstractDownload PDF (3799K) -
Kohsuke CHIDA1993 Volume 70 Issue 4 Pages 151-155
Published: October 20, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSAlkaline phosphatase (ALP) in rat liver was detected by means of immunohistochemical techniques in ordinary histologic specimens which were fixed with formalin and embedded in paraffin. The specimens were incubated in anti-ALP antibody at room temperature for a longer time,3 hr, than ordinary incubation time. Antigen detection sensitivity and contrast of reaction product of the immunogold silver staining were superior to those of the peroxidase-labeled antibody method. In rat livers which were partially hepatectomized 24 hr before, the more abundant enzyme localized around enlarged bile canalicular spaces.View full abstractDownload PDF (1451K) -
Iwao SATO, Kazuyuki SHIMADA, Tooru SATO, Richard ETHERIGE1993 Volume 70 Issue 4 Pages 157-163
Published: October 20, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe functional teeth of adult salamander (Caudata): Ambystoma maculatum, Salamandra salamandra and Aneides lugubris were prepared for transmission electron microscopy and electron microprobe analysis of the elements and trace elements of the enamel layer. The arrangement of enamel crystals in these species is variable. The outer part of the enamel layer in which hydroxyapatite elements (Ca, P) and trace elements (e.g.,F,Fe,Mg) are concentrated, is most mineralized. The concentration of trace elements are arrangement of crystals in the enamel layer differed in these species, may be related to the pattern of mineralization of enamel.View full abstractDownload PDF (3138K) -
Atsumi UKESHIMA, Masayuki KUDO1993 Volume 70 Issue 4 Pages 165-169
Published: October 20, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe intraembryonic blood islands preceding the hepatic haemopoiesis, were examined in embryos from stage 17to 19 by light microscopy, and their three-dimentional expansion was demonstrated. These blood islands were located in the mesenchyme of the lateral fold, mesentery, allantois and ventral aorta, commonly showing large scale. Except the lateral fold, however, those were just temporary sites. Particularly large islands were necessarily found in the mesenchyme of the lateral fold at the stages examined. They were present bilaterally and exhibited cephalocaudally long, rod-like structures with variable sizes. The largest island measured over 2,600 μm in length.View full abstractDownload PDF (1392K) -
Atsumi SHIMOZAWA, Hiroaki NAKAMURA1993 Volume 70 Issue 4 Pages 171-180
Published: October 20, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSNerve fiber counts and caliber analysis of the nerve branches to m. mylohyoideus were performed with an electron microscope on 5 sides from 3 mice. The nerve branches contained an average of 93.6 (54.7%) myelinated fibers,76.2 (44.6%) unmyelinated. fibers and 1.7 (0.7%) undetermined fibers.60.3% of the myelin sheaths of the myelinated fibers in the nerve branches were3476.0 μm in major diameter and 67.8% were 2.5-5.0 μm in minor diameter.81.7% of the axons of the myelinated fiberk'rveie 1.5-4.5 μm in major diameter and 83.1% were 1.0-3.5 μm in minor diameter. The diameter frequency distributions of the myelin sheaths and axons of myelinated fibers in the nerve branches were similar in pattern to that of the facial trunk distal to the stylomastoid foramen. On the other hand, these frequency distributions of the nerve branches showed that the diameters of the myelinated fibers of the nerve branches were approximately 1-2 μm smaller than those of the r. digastricus, r. stylohyoideus and the nerve branch to venter rostralis of m. digastricus. Average ratios of the cross-sectional areas of myelin sheaths to those of axons of the myelinated fibers in the various axon area classes were 0.59-1.18, and those ratios tended to decrease in the larger axon classes.View full abstractDownload PDF (2839K) -
Hidekazu WAKURI, Kazutoshi KAWAMUR, Ken-ichiro MUTOH1993 Volume 70 Issue 4 Pages 181-193
Published: October 20, 1993
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSIn six pigs from 7 months to 3 years of age the cleavage line pattern of the skin was examined in the whole body by a thick metal probe with a sharp conical point. Directionality of the cleavage lines was determined from the relationship of the line of the long axis of each physical structure. The cleavage lines of adult pigs were compared with those of young pigs (Wakuri,1967). The cleavage lines of the ear, neck, trunk, and free extremities of appendage were quite similar in adult and young pigs. Those of the buccal, interscapular and sacral regions, umbilicus and scrotum showed slight differences. The most noticeable directional variability in cleavage lines appeared in the masticatory region, arms and legs of adult pigs. Cleavage lines were found to be transverse in arrangement in the masticatory region, auricular root, neck, trunk, tail and legs. They showed an annular pattern around the preputial orifice and in the arms and anogenital region.View full abstractDownload PDF (3154K)
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