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Volume 21 (1968)
- Issue 6 Pages 369-
- Issue 5 Pages 301-
- Issue 4 Pages 239-
- Issue 3 Pages 173-
- Issue 2 Pages 95-
- Issue 1 Pages 1-
Predecessor
Volume 21, Issue 5
Displaying 1-7 of 7 articles from this issue
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SEIYA KOHNO, MASAYOSHI KOHASE, MASARU SUGANUMA1968 Volume 21 Issue 5 Pages 301-311
Published: 1968
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSMeasles virus was found to grow in L cells, without producing cytopathic effects. The growth curve experiments revealed two phases of infection, viz., the early logarithmic multiplication and the later persistent state of infection. Studies on viral penetration and uncoating (or disintegration) demonstrated the relatively low efficiencies in both reactions. Release of the progeny viruses was also suppressed. They were found mostly in the extreme neighborhood of the cell membrane.
Most of the cells at the later phase of infection were susceptible to measles virus, when tested after cell-cloning. They were carrying no virus or virusrelated antigens (as tested by subculture with mixed Vero cells, or by fluorescent antibody staining and hemadsorption) .
These findings, altogether, indicate a suppression in spreading of the viruses in the culture.
When the cultures of the later phase of infection were treated with PH 3.0 for 5 minutes, the total cell-associated infectivity dropped drastically. But subsequently, without a lag, a prompt recovery of infectivity was observed.View full abstractDownload PDF (1040K) -
II. SEROLOGICAL CHARACTERISTICSRIICHI SAKAZAKI, SETSUO IWANAMI, KAZUMICHI TAMURA1968 Volume 21 Issue 5 Pages 313-324
Published: 1968
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESS1. A total of 2720 cultures of Vibrio parahaemolyticus isolated from human patients was studied serologically. By agglutination and agglutinin-absorption tests, eleven O groups and 47 K antigens, of which 6 were omitted from the antigenic schema, were designated.
2. Since it was confirmed that all cultures of the species possess a common H antigen, the antigenic formula of serotypes was designated with a combination of O and K antigens.
3. In some K antigen, quantitative variation of partial antigens was recognized, and the designation of these partial K antigens was omitted from the antigenic schema to simplify it.
4. An antigenic schema was set up for 41 serotypes of the vibrio.
5. Additional 650 cultures isolated from sea fish and sea water were tested with the eleven O and 41 K antisera. The occurrence of individual serotypes in human cultures and cultures from sea fish and sea water was compared.
6. Methods of a diagnosis of serotypes are discussed.View full abstractDownload PDF (1174K) -
III. ENTEROPATHOGENICITYRIICHI SAKAZAKI, KAZUMICHI TAMURA, TEIJI KATO, YASUSHI OBARA, SHIRO YA ...1968 Volume 21 Issue 5 Pages 325-331
Published: 1968
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSIn recent years, it was demonstrated that cultures of Vibrio parahaemolyticus isolated from human diarrheal stools were different from those from sea fish and sea water in a hemolytic activity on a special blood agar, which was named the Kanagawa phenomenon. In the present studies, the finding has been confirmed by using 3370 cultures of the vibrios. Ninety-six % of the vibrios isolated from human patients gave positive (hemolytic) reaction in the test of the phenomenon, while only one % of the vibrios from sea fish and sea water gave positive results in the test. No relationship was observed between the phenomenon and serological and biochemical characteristics.
Enteropathogenicity of the cultures of the vibrios positive in the Kanagawa phenomenon was evident by the results of feeding tests according to Takikawa and Aiiso and Fujiwara and by a case of laboratory infection of the culture to a male who took gastroenteritis by administration of approximately 106 viable cells of the hemolytic vibrios in the test of the phenomenon. On the other hand, feeding tests were carried out with 15 human volunteers to clarify enteropathogenicity of the negative (non-hemolytic) vibrios. None of them took illness, although over 109 cells of the vibrios were administered. Thus, the authors considered, as a rule, that the vibrios giving negative Kanagawa phenomenon may be nonpathogenic to human beings.View full abstractDownload PDF (793K) -
HIDEMASA OGAWA, AKIKO NAKAMURA, RIICHI SAKAZAKI1968 Volume 21 Issue 5 Pages 333-349
Published: 1968
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSThe “enteropathogenicity” of Escherichia coli serotypes isolated from diarrheal adults and children was examined by three bioassay systems ; inoculation into guinea pig eyes, into ligated rabbit intestine, and to cultured HeLa S3 cells.
The OK types, 0144: K ? (B), 0143: K ? (B), 0136: K78, 0124: K72, and 028a, c: K73, were isolated from the patients of dysentery-like symptoms and designated as Shigella-like group. These strains, except one old laboratory strain, were positive in the three bioassays causing keratoconjunctivitis in guinea pigs, dilatation of the rabbit intestinal segments, and intracellular multiplication within HeLa cells.
On the other hand, the OK types, 026: K60, 044: K74, 055: K59, 0119: K69, 0126: K71, 0128: K67, 0146, K86, 086a : K61, and 086a : K62 were isolated from the patients of gastroenteritis symptoms and designated as Salmonella-like group. All the strains of this group were completely negative in the three bioassay systems.
The ablity of Shigella-like E. coli to penetrate into the cells of intestinal epithelial lining was interpreted as the indispensable factor for establishment of the infection, as was the case with Shigella bacilli.View full abstractDownload PDF (12644K) -
NAOKO NAKANO1968 Volume 21 Issue 5 Pages 351-357
Published: 1968
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSA toxic substance named fusarenon was isolated from rice grains infected with Fusarium nivale. The substance, at concentrations of 50μg/ml or higher inhibited completely the division of Tetrahymena pyriformis (ciliated protozoan) in exponentially growing mass culture and in temperature-induced synchronous culture.
From the delay in cell division caused by exposure of the cells to 200μg/ml of f usarenon for 20 min at various stages after the end of the synchronous treatment, it was shown that the cells at 30 min before the synchronous division maximum were most susceptible to the toxic substance. The cells at this stage are known to have completed the syntheses of DNA and m-RNA and be actively synthesizing the specific protein, all of which are indispensable with the forthcoming division.
From pulse-labeling with 3H-thymidine, -uridine or -phenylalanine in the presence of fusarenon at different concentrations, it can be said that fusarenon affects most seriously the protein synthesis. It is, therefore, likely that the blockage of the cellular protein synthesis caused by the toxin may result in the inhibition of cell division.View full abstractDownload PDF (569K) -
RIICHI SAKAZAKI1968 Volume 21 Issue 5 Pages 359-362
Published: 1968
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSDownload PDF (304K) -
SABURO IWASA, SETSUJI ISHIDA, SADAO ASAKAWA, MASAMI KUROKAWA1968 Volume 21 Issue 5 Pages 363-368
Published: 1968
Released on J-STAGE: March 19, 2010
JOURNAL FREE ACCESSDownload PDF (563K)
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