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Volume 68 (1991 - 199･･･
- Issue 6 Pages 319-
- Issue 5 Pages 259-
- Issue 4 Pages 215-
- Issue 2-3 Pages 81-
- Issue 1 Pages 1-
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Volume 68, Issue 5
Displaying 1-6 of 6 articles from this issue
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Akira YASUNAGA, Seiji KATO, Yuzou UCHIDA, Ryosuke MIYAUCHI1991 Volume 68 Issue 5 Pages 259-269
Published: 1991
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe fine distribution of the intramural lymphatics at the ileocecal junction of the monkey intestine, especially in the lamina propria of the ileocecal valve, was examined by light and electron microscopy using enzyme-histochemical staining. The distinction between the lymphatics and the blood vessels was made by light microscopy on cold glycol methacrylate resin (JB-4) sections using 5'-nucleotidase (5'-Nase)-alkaline phosphatase (ALPase) double staining. The lymphatics were found to show strong 5'-Nase activity and to comprise irregularly shaped vessels or spaces. The central lymphatic vessels (central lacteals) in low villi were seen to lie deep within the ALPase-positive subepithelial capillary network. In the ileum side of the ileocecal junction, the 5'-Nase-positive lymphatics were seen both in the superficial layer and the deep layer of the lamina propria. On the contrary, in the cecum side the mucosal lymphatics were less numerous in the superficial layer and were distributed mainly in the deep layer near the lamina muscularis mucosae. These lymphatics ran through the lamina muscularis and merged into the lymphatic network in the submucosa. The submucosal lymphatics communicated with each other at the ileocecal junction and formed a well-developed network. Collecting lymphatics with valves were also seen near the tunica muscularis (sphincter muscle) in the deep submucosa. These lymphatics traversed the muscle layer and drained into the subserosal lymphatics.View full abstractDownload PDF (6307K) -
Chiaki YAMAMOTO, Etsuro KAWANA1991 Volume 68 Issue 5 Pages 271-282
Published: 1991
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSTo study MPTP-induced muscular rigidity, we try to detect the changes of both dopamine (DA) and GABA within rat striatums by immunohistochemical means. A high dose (30 mg/kg) of MPTP i. p. injected into rats produces behavioral abnormalities (tremor and ataxia), and higher doses (>60 mg/kg) develop an acutely muscular rigidity without producing a measurable histological change. GABA is induced in the striatum of 4 MPTP (30 mg/kg) i. p. treated-rats developed tremor and ataxia. But the animals recovered to an apparently normal state and are not showed GABAimmunoreactivity. Dense GABA-immunoreactivity is observed in the striatum developed muscular rigidity, when the animals are injected with 60 mg/kg MPTP i. p.. At this time, their striatums show slight decrease of DA-immunoreactivity in medium sized-spiny neurons. The results give some insight as to how DA and GABA function within the striatum with respect to the development of neuronal abnormalities. It is also suggested by our behavioral and immunohistochemical studies that effects induced by the depletion of DA within the striatum may be mediated through the inhibition of the striato-nigral GABAergic pathway, which in turn may lead to an activation of the nigrothalamic or nigro-collicular GABAergic pathway. This study indicates that the role of striatal GABAergic transmission is important in the development of muscular rigidity. The activity within the striatum can be followed with immunohistochemical technique for GABA morphologically.View full abstractDownload PDF (5774K) -
Akira NAITO, Yoshifusa SHIMIZU, Yasunobu HANDA, Masayoshi ICHIE, Nozom ...1991 Volume 68 Issue 5 Pages 283-288
Published: 1991
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSActivities of the elbow flexors and extensors during the movement of the elbow flexion and extension were analyzed in six normal human volunteers by electromyography (EMG). In the flexors, the majority of the muscles showed EMG activities during both the flexion and extension phases, although patterns and amplitudes of EMG activities varied from individual to individual. The biceps brachii always became less active when the forearm was in pronation. In the extensors, increase of EMG activities was observed at the period of the maximum elbow extension in the majority of cases, while no EMG activity was shown throughout the movement in some cases. During the elbow movement except at the maximum extension, the triceps brachii was almost inactive and some of their three heads, in particular the long head, often showed no EMG activity. In contrast, the anconeus was usually active, sometimes showing strong EMG activity.View full abstractDownload PDF (1823K) -
Masatake IMAI, Taizo SHIBATA, Keiichi MORIGUCHI, Hiroyuki HAYAMA1991 Volume 68 Issue 5 Pages 289-293
Published: 1991
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSIn an earlier study, we found compound tubular glands distributed in the lamina propria mucosae of human and fowl esophagus.
Subsequently, we discovered bottle-shaped glands in the Japanese lizard and gecko esophagus in the same lamina as that of the human and fowl. Moreover those glands produced equivalent pepsinogen granules. We provide below, a detailed description on the results.
1. Bottle-shaped glands were distributed in the lamina propria mucosae of the Japanese lizard and gecko esophagus.
2. A large number of those glands were distributed in the lower region of the esophagus, but did not exist in the upper and middle regions of the esophagus.
3. The esophageal mucous membrane of the gecko and Japanese lizard were covered with a simple columnar ciliated epithelium, and the same epithelium reacted strongly to PAS and AB (pH 2.5), moderately to AB (pH 0.5) or negatively.
4. PAS-AB (pH 2.5) stain presented a dark blue color or a deep red color or a deep red and dark blue mixed color in one section.
5. The above-mentioned glands contained pepsinogen granules.
6. Those glands do not possess parietal cells.View full abstractDownload PDF (1907K) -
Hideki SAKAI, Kuniaki TAKATA, Minoru FUKUDA, Takaaki FUJIWARA, Hiroshi ...1991 Volume 68 Issue 5 Pages 295-298
Published: 1991
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSAn improved fixation technique for transmission electron microscopic observation that enables good fixation of all areas of the rat lens was devised. Immersion fixation with 0.1 M phosphate-buffered 1.25% glutaraldehyde at 4°C for 12 hours followed by postosmication produced good results for all areas of the lens - anterior, equatorial, and posterior zones. The technique was particularly suitable for maintenance of the shape of the lens since practically no irregularity, vacuolar degeneration, or expansion of the intercellular spaces was noted. This technique, which requires only a relatively short time, was also useful for the detection of early ultrastructural changes associated with cataract in spontaneously diabetic WBN/Kob rats. We anticipate that our procedure will be widely applied.View full abstractDownload PDF (1945K) -
Yasukazu NAGATO, Masaki SEKIGUCHI, Hiroshi KUSHIDA, Kazuyo SHIMAI1991 Volume 68 Issue 5 Pages 299-304
Published: 1991
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSTo facilitate improvement of investigations on the distribution of mossy fibers in the hippocampal formation, a method is described using Timm's stained preparations after methacrylate embedding with the hydrophilic resin, Quetol 523M. Fixation with a mixture of formaldehyde and glutaraldehyde yielded satisfactory staining results and good structural preservation. During the course of histochemical experiments employing Timm's staining, examinations revealed that sulfide silver reaction products were consistently present in both the mossy fibers themselves and their terminals associated with the dendrites of pyramidal cells in tissue sections of 1-2μm in thickness. The results obtained also revealed that variations of the mossy fiber system occurred in the neurological mutant mouse dreher (dr). The bundles of mossy fibers forming the intrapyramidal synaptic field may be considered to reflect genotype-dependent differences in the mutation. The present method is adequate for allowing the histochemical demonstration of mossy fibers and their giant boutons by light microscopy.View full abstractDownload PDF (2527K)
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