We have developed a high-resolution electron cryo-microscope by which images can be recorded with higher resolution than 3Å at a specimen-stage temperature of 4.2K. By using this instrument and electron crystallography the structures of Light-harvesting complex and bacteriorhodopsin were analysed at resolutions of 3.4Å and 3.0Å, respectively. The structure analysis of LHC gave the coordinates of twelve Chlorophyll molecules and two Lutein ones together with those including loops of the polypeptide chain. The result of bR resolved analyse atomic structurre from two dimensional crystals.
抄録全体を表示