生物物理
Online ISSN : 1347-4219
Print ISSN : 0582-4052
ISSN-L : 0582-4052
54 巻, 5 号
通巻315号
選択された号の論文の16件中1~16を表示しています
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  • 新海 暁男
    2014 年54 巻5 号 p. 247-252
    発行日: 2014年
    公開日: 2014/09/30
    ジャーナル フリー
    Adaptive immunity is acquired when foreign substances enter the body. Interestingly, this immune system exists not only higher organisms but also many bacteria. The CRISPR-Cas systems are the bacterial adaptive immune systems against foreign DNA and RNA derived from phages or plasmids. Many bacteria can memorize the sequences of foreign DNA and RNA, and remove them by means of these systems. Structural and functional studies of these systems have dramatically progressed over the past few years. In particular, one of the CRISPR-Cas systems, Cas9, has received considerable attention as a highly efficient tool for genome editing.
総説
  • 清中 茂樹, 坂口 怜子, 森 泰生
    2014 年54 巻5 号 p. 253-256
    発行日: 2014年
    公開日: 2014/09/30
    ジャーナル フリー
    Thermoregulation to conserve body temperature is vital to life in all animals. Multiple mechanisms localized in subcellular organelles have been proposed for thermogenesis. However, direct observation of thermogenesis in intact organelles has not been achieved yet. Recently, we developed genetically-encoded thermosensors ‘tsGFPs’. In tsGFPs, a tandem formation of coiled-coil structures of the Salmonella thermosensing protein, TlpA, transmits conformational changes to the inserted GFP to convert temperature changes into visible and quantifiable fluorescence changes. In mammalian cells, specific targeting of tsGFPs visualized thermogenesis in discrete organelles, notably mitochondria in brown adipocytes and the endoplasmic reticulum in myotubes.
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