Site specific incorporation of unnatural molecules into native proteins are proven to be one of the most promising methodologies in protein molecules engineering. In this article, I describe successful examples of function regulation of hemoproteins by interactions with phenylboronic acid as a sugar recognition site and maleic acids as controlling sites of electrostatic repulsion. As another approach, molecular assemblies such as lipid bilayer membranes, are demonstrated to be potential one which enable the functional conversion of the bound-protein through noncovalent but domain-specific perturbations.
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