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Volume 19 (1964 - 196･･･
- Issue 6 Pages 341-
- Issue 5 Pages 285-
- Issue 4 Pages 233-
- Issue 3 Pages 169-
- Issue 1 Pages 1-
Volume 19, Issue 6
Displaying 1-5 of 5 articles from this issue
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Nozomi Takemura1965 Volume 19 Issue 6 Pages 341-348
Published: February 10, 1965
Released on J-STAGE: February 17, 2009
JOURNAL FREE ACCESSIt can be imagined that naturally occuring environmental carcinogens have existed in the human environment since long ago. For example, aflatoxin, a potent carcinogen, found recently in Brazilian peanuts suggests this. On the other hand, the incidence of hepatoma of rainbow trout in hatcheries throughout the United States, of which the cause is attributed to the artificial ration given to the fish, suggests that similar risk may occur in the human because of the increase of chemical productions, as Hueper stated.
The high incidence of cancer in an organ of a certain race should not be considered to be entirely genetic of that race. For if it had been considered so, some important social environmental factors which might have effect indirectly on the incidence would have escaped our attention and the study of cancer itself would not have made progress.
It is stressed by Poel and others that cancer is a terminal stage of normal cell proliferation under inadequate homeostatic restraint. In fact, many functional stages in the humen body have relation wtoh and effect on its abnormal progressive proliferation of normal cells. Furthermore etiology of environmental cancer is so complex that it is desirable to advance epidemiological, pathological, biochemical and molecular-biological researches further and deeper together for the better understanding of the nature of environmetal carcinogenesis. The problems of cancer of the skin, stomach, lung, bladder and breast in the human have been discussed from these viewpoints.View full abstractDownload PDF (1283K) -
Yasushi Kodama1965 Volume 19 Issue 6 Pages 349-364
Published: February 10, 1965
Released on J-STAGE: February 17, 2009
JOURNAL FREE ACCESSExperimental studies were performed on the formation of carcinogenic 3, 4-Benzpyrene in meat treated under pyrolytic conditions.
(1) As the temperature to which meat is subjected in usual cooking is 300°C to 500°C, the experimental pyrolysis of meat was made at 300°C and 500°C in both aerobic and a nitrogen atmosphere and at 700°C in a nitrogen atmosphere.
(2) Chemical analysis was performed in the following manner on the tarry material obtained from the scorches of meat and the smoke generated, respectively. One kilogram of minced meat was first dried at 110°C for 3 hours, and pyrolysed at the specific temperature. The smoke generated was absorbed in a series of bottles containing aceton or benzene. The solvent was evaporated off under reduced pressure in a nitrogen atmosphere to get a tarry material. The scorches were seperated and extracted with aceton in a Soxhlet apparatus for several hours. The aceton extract was then evaporated off to obtain a tarry material.
(3) The tarry material obtained from smoke was washed with 10% NaOH, 10% H2SO4 and water. The neutral fraction of tarry material thus obtained was chromatographed repeatedly on activated alumina until it decreased to about 0.5g. Benzene, cyclohexane, and petroleum ether containing 5% acetone were used as eluents.
(4) The final column chromatogram was carried out using alumina containing 3% H2O as adsorbent and cyclohexane followed by cyclohexane containing 10 to 30% benzene as eluent. The purification of 3, 4-benzpyrene and other polycyclic aromatic hydrocarbons was made by paper chromatography using acetylated paper. The polycyclic aromatic hydrocarbons contained in each eluate fraction were identified and determined by the absorption spectrophotometry reported by Cooper.
(5) The amounts of 3, 4-Benzpyrene detected in the smoke and scorches obtained by pyrolysis of meat (1kg. wet material) were as follows:
4580μg and 132μg at 700°C under N2, 31μg and 16μg at 500°C under N2, and 2μg in smoke at 500°C under the ordinary atmosphere. No 3, 4-Benzpyrene was detected in the tarry material obtained under other experimental conditions.View full abstractDownload PDF (2163K) -
Especially on the sensitized-erythrocyte lysis test and the fluorescent antibody testSusumu Imamura, Yoshio Ashizawa1965 Volume 19 Issue 6 Pages 365-368
Published: February 10, 1965
Released on J-STAGE: February 17, 2009
JOURNAL FREE ACCESSNumerous attempts have been reported by many workers to improve the practical methods in the serodiagnosis of leptospirosis. Efforts were made to find diagnostic test with high sensitivity and specificity that is easily performed without the use of living leptospirae.
The leptospiral sensitized-erythrocyte lysis test developed by Chang and others was studied by us using freeze-dried antigen obtained from sonically disrupted cells and the results were compared with those of the agglutination-lysis test. The test was observed to be a valuable diagnostic procedure.
Studies were also made on the indirect fluorescent antibody test and the results were also compared with those of the agglutination-lysis test. Test results with the 2 procedures showed good correlation, but the sensitivity of the fluorescent antibody test was lower than that of the agglutination-lysis test.View full abstractDownload PDF (481K) -
Part 1. Chages in the ammonia content of brain, liver, and blood under some environmental conditionsChikara Sakaguchi1965 Volume 19 Issue 6 Pages 369-373
Published: February 10, 1965
Released on J-STAGE: February 17, 2009
JOURNAL FREE ACCESSThe studies were carried out to investigate the changes in the ammonia content of brain, liver, and blood under different physical environments. The environmental conditions studied were noise, cold, and immobilization.
The adult male rats were exposed to 100∼105 phone noise characterized by wide octave band spectrum for 3 hours, or to cold (-10±1°C) for 3 hours, or were immobilized for 2 hours. There-after, the animals were killed by two different methods; the one was decapitated at room temperature and the other was dropped into the mixture of solid carbon dioxide and acetone. The brain and liver were rapidly removed, and homogenized in ice-cold 10% trichloroacetic acid with glass homogenizers. The blood samples were obtained by heart puncture. The determination of ammonia was made by Conway's method using Seligson-Hirahata's apparatus.
As the results, under the noise condition, the ammonia content was elevated significantly in the brain, liver, and blood, but not under the other conditions.
It may be concluded that the noise acts not as metabolic stimuli but as neurotropic stimuli with a peculiar nature.View full abstractDownload PDF (715K) -
1965 Volume 19 Issue 6 Pages 380
Published: 1965
Released on J-STAGE: February 17, 2009
JOURNAL FREE ACCESSDownload PDF (60K)
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