The authors investigated effects of fluctuating noise on hearing. Seven persons were experimentally exposed to a steady state noise (S) of 98 phon for 60 minutes, a fluctuating noise (A) of 101 phon with standard deviation of 8.8 phon for 40 minutes and (B) of 99 phon with s.d. of 7.0 phon for 60 minutes. The fluctuating noises were composed of air exhaust noises.
All values were measured using JIS method. The TTS was measured five times, in time series of exposure and showed in dB value.
Regression analysis applied to the data obtained revealed statistically significant linearity between the increase of TTS and the exposed duration in minutes in common logarithm, with all three kinds of noise as follows;
Y_S=9.69log₁₀X+6.29 Y_S, Y_A, Y_B......TTS in dB with noise S, A and
Y_A=32.12log₁₀X-16.14 B respectively
Y_B=19.17log₁₀X-5.24 X......exposed duration in minutes
The difference among the three regression coefficients was significant at 5% level with each other. These results indicate that TTS increases linearly parallel with exposed duration in minutes in common logarithm and that the greater TTS with noise A than that with noise B and similarly the greater TTS with B than that with S are observed. Since the three means of OBL on frequency analysis with the three noises around the center frequency of 3kc were nearly the same value, the present study may indicate that the TTS due to a fluctuating noise is greater than that due to a steady state noise and that the TTS may be modified by the characteristics of the fluctuating noise.
As to the TTS recovery term, longer duration was observed in cases of greater TTS as Ward et al and Yamamoto et al indicate.

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It was observed that there are changes in blood glucose levels induced by inhalation of lower concentration of carbon monoxide (CO) in rabbits.
The previous report was designed to ascertain the pattern of action of CO:, the method used was intravenous injection of saline dissolving CO (CO-saline) or red blood cell suspension saturated with CO.
In this report experimentation was performed to compare the action of CO with that of adrenaline on the changes in the blood glucose. Effect of phentolamine and propranolol was also studied in this experiment, and a part of the experiment was performed in reserpinized and spinal rabbits.
Results obtained were as follows:
1) The changes in blood glucose levels after CO-saline injection was not influenced by the administration of phentolamine or propranolol.
2) In the case of reserpinized rabbits, changes in the blood glucose by CO-saline were not observed, but the effect turned to be positive after repetitive injection of adrenaline.
3) In the spinal rabbits, injection of CO-saline failed to cause changes in blood glucose levels while injection of adrenaline increased.
The findings indicate that the changes in blood blucose may be elicited by the central action of free CO and this change induced by CO-saline was not directly related to α and β receptors.
It is suggested that there may exist another glycotic mechanism, different from hyperglycemia as a result of adrenaline being released from peripheral organs such as the adrenal medulla.

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Most houses in Japan were provided with cesspool- or septic tank-percolation field system for domestic sewage disposal. The author carried out a two-year test on biodegradation of linear aklylate sulfonate (LAS) under the cesspool-percolation field, prior to the detergent conversion to biodegradable surfactants. Good drainage was sustained with the test LAS cesspool in comparison with the former ABS cesspool; so the test cesspool-percolation field system maintained an unsaturated soil condition.
Under aerobic and anaerobic conditions, analysis on bacterial flora of the LAS cesspool was carried out on soil and waste taken from the cesspool. Among LAS assimilable bacterial species as Micrococcus, Aerobacter, Flavobacter, Paracolobactrum, Alcaligenes and Pseudomonas, a Pseudomonas sp. appeared to play the main role in the process of surfactant biodegradation. The Pseudomonas was named as Pseudomonas surfactassimilas nov. sp..
Taxonomical characteristics of Pseudomonas surfactassimilas nov. sp. are as follows;
The strain is Gram-negative rods and motile with a polar flagellum. Branching is not found.
Rods: 0.4 to 0.6 by 1.2 to 1.5 microns.
Nutrient agar colonies: Circular, smooth, entire, flat, yellowish gray.
Nutrient agar slant: smooth, glistening, transluent, yellowish gray, medium unchanged.
Potato: Growth moderate, creamy to yellowish gray.
Litmus milk: Unchanged.
Aerobic, facultative.
Non fluorescence on King's media.
Catalase: Negative.
Kovacs' oxidase test: Positive.
Gelatin Stab: No liquefaction.
Nitrates: Not reduced.
Acetylmethyl carbinol: Not produced.
Urease: Negative.
Indol: Not produced.
Hydrogen sulfide: Not produced.
Ammonium citrate: Not utilized.
Starch: Not hydrolyzed.
Casein: Not hydrolyzed.
Cellulose: Not attacked.
Glucose, lactose, sucrose: Not fermented.
Citric acid, tartaric acid, succinic acid, acetic acid, fumaric acid: Used as a sole source of carbon.
Valeric acid, propionic acid, butyric acid: Not utilized as a sole source of carbon, but used under existence of vitamins.
Phenol (1%): Growth observed after twice inoculation on synthetic broth as a sole source of carbon.
Surfactants with straight alkyl chain: Utilized as a sole source of carbon.
Temperature relations: 20-37°C, optimum 30°C.
Optimum pH: 7.0
Source: Isolated from soil and waste of cesspool.
When waste water was treated under aerobic circumstances, surfactants with straight alkyl chain could be decomposed by biological oxidation to the extent comparable with, or more than, the degradation of general organic substances. The cesspool-percolation field system easily maintained an unsaturated condition to avoid excessive ponding in the percolation field by periodic supply of waste water which provided aerobic condition.

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There was a relatively high incidence of group O persons among those affected by influenza A2 infection as previously reported by McDonald and Zuckermann. However, experimental proof of their statistical survey has not been obtained. The object of this study was to show the participation of ABO blood group substance and its antibody in influenza A2 infection.
(1) Blood group substances of egg grown influenza viruses were studied by means of the agglutinin-inhibition test and the elution test. Concentrated, washed and methanol treated suspension of influenza viruses did not inhibit the activities of anti-A, anti-B and anti-H agglutinins. The elution test was then carried out by means of using the virus particles adhered on paper strips. The strips were sensitized with sufficient amounts of anti-A (human), anti-B (human) or anti-H (Ulex europaeus) at a low temperature, and washed with chilled normal saline. Excessively bound agglutinins were then liberated from the strips by heating up to 50°C for 20min. The presence of only anti-H agglutinin was proved in thus obtained eluates. This result indicates the content of blood group H substance in influenza A2 virus. The H substance in influenza B virus was also certified by this test method, but its quantity was assumed to be less than that of A2. For the purpose of assuring the H substance, chickens were immunized with concentrated influenza A2 (Hongkong) virus with incomplete Freund's adjuvant. In the sera of 3 out of 5 chickens, a relatively weak complete anti-H and Eisler's agglutinin were determined to have been produced.
(2) After the cessation of influenza A2 (Hongkong) epidemic in 1969, 455 individuals' adult human blood samples were collected to determine blood groups, the presence or absence of complete anti-H agglutinin and the titer of hemagglutination inhibition (HI) titer for A2 (Hongkong) virus.
Results obtained were:
1) No correlation was found between the range of HI titer and the classification of blood groups, ABO, MN, P, Rh and Lewis.
2) Incidence of complete anti-H agglutinin in influenza vaccine recipients' and non-recipients' sera were very similar.
3) Incidence of complete anti-H agglutinin and HI titer in the sera of influenza A2 (Hongkong) vaccine non-recipients was compared. Frequency of the sera showing HI titer of 1:4 or higher were less in the group of anti-H positive group relative to the anti-H negative group. X² test of the result, however, showed no significant difference. (Probability≈0.1)
(3) Based on the results of the present study, it was assumed that the anti-H agglutinin in nasal or tracheal secretions of group A, B and AB persons could adhere to H substance of influenza A2 virus to inhibit its growth.

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(I) Various techniques in agglutination methods using passively sensitized particles were compared for the purpose of determining a suitable test method for detecting small amounts of univalent antibodies toward soluble substances in chicken eggs.
1) Univalent anti-egg albumin (-EA) antibody, produced in primary response immune sera of rabbits, proved its serological activity in all three agglutination tests of EA-sensitized particles, i.e., passive hemagglutination, bentonite agglutination and latex agglutination.
2) A tube technique using latex particles sensitized using small amounts of egg materials was determined to be the method best suited for this study.
3) Appropriate concentrations of EA, egg yolk (EY) and arantoic Fluid proteins (AF) to sensitize latex particles were determined.
(II) Sera from influenza vaccine recipients and nonrecipients were tested by latex agglutination using particles sensitized with EA, EY and AF. Results obtained were as follows:
1) In all 589 recipients' sera, 107 (18.17%) samples proved to contain one or more kinds of univalent antibodies toward chicken egg materials. Only 3 (1.17%) 237 non-recipients' showed positive reactions to the antibodies.
2) Nonspecific latex agglutination occurred in the tests of 36 (6.11%) samples of sera from the recipients. In the remaining 553 samples, 48 (8.86%) proved anti-EA activity, 70 (12.66%) for anti^* EY and 51 (9.22%) for anti-AF respectively. Relatively high incidence of anti-EY was estimated partially due to the nonspecific agglutination of EY-sensitized particles.
3) Titers obtained by latex agglutination methods were lower than those by passive hemagglutination test. However, it was found that these two test methods showed similar rates of antibody detected sera due to the relatively low incidence of nonspecific latex agglutination.
4) Antibodies against egg materials in recipients' and nonrecipients' sera revealed serological reactions by neither ring precipitin test nor two-dimensional immunodiffusion using cellulose acetate membrane.
5) No statistical relationship was found between frequencies in production of anti-egg material antibodies and the classification of some blood groups, ABO, MN, P and Rh (D).
6) No correlation was found between the presence in sera of anti-egg material antibodies and HI antibody titer toward influenza/Hongkong virus which was an ingredient of the vaccine.

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Heights and weights of infants (142 boys and 117 girls) who were physically examined at an infant health center in Osaka Prefecture during 1964 and 1967 were followed up monthly for one year from birth. The following results were obtained by the analysis of the data.
1) The average height and weight of the male and female infants investigated were slightly larger at every age of month than the standard set by the Ministry of Welfare in 1960. However, the infants investigated can be considered as a relatively unbiased sample of the population of Japanese healthy infants at least as far as size and growth of body build, as the body build of infants during the period of the investigation were slightly larger than those in 1960.
2) Relatively large positive correlations were found between height (weight) of a infant measured at different age of month. Moreover, when male and female infants were classified into three groups (large, normal, small) according to their height (weight) at the time of birth, and height (weight) of each group was followed up for a year, it was found that in both boys and girls, the absolute values of difference of mean height (weight) among three groups at the time of one year old was almost the same as that observed at the time of birth.
3) However, a relatively large variation of relative body size of a infant at different month ages and large coefficients of variation observed on the monthly increasement of height (weight) in every interval between two successive age of month suggested that there were relatively large individual variation in the growth of infants.
4) Using Rao's method, the standard growth curves of height and weight of infants and their 99% confidence bands were obtained for groups of male and female infants classified by their height (weight) at the time of birth, on the assumption that the deviations of any individual growth process from population growth curve have a multivariate normal distribution. It was found that in every group, the standard growth curve of height (weight) was expressed in the three degree polynomial in age of month and it corresponded well with the observations.

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Along with development of mechanical analysis, various methods have being applied for determination of total mercury. The method generally used however is colorimetry by dithizone extraction. Reproduction and accuracy were poor owing to low sensitivity, and this method requires great quantities of samples and reagents. As for dithizone extraction, interferences from other metals and the instability of color have resulted in measurement errors.
The purpose of the present paper is to establish a simplified method of total mercury determination with high sensitivity and good reproduction.
Determination was performed in the following proceduers,
1) Treat solid samples by combustion method, and use digestion at room temperature or reduction method when the samples are liquid.
2) Collect mercury in 0.2% KMnO₄-1N⋅H₂SO₄ solution using midget impinger.
3) Transfer this solution to separating funnel with 1ml of 40% NH₂OH⋅HCl and extract by 0.002% dithizone chloroform.
4) Take a part of dithizone chloroform layer to combustion boat, and vaporize mercury in quartz tube at 700°C.
5) Determine generated mercury vapor by ultraviolet atomic absorption using spectrophotometer.
6) Recovery rate was 99.3%, detectable range was 0.0003μg to 0.2μg and a linear relation was observed in this range.

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A considerable number of reports have been published on the experimental symptoms of poisoning by mercury. There have however been very few reports on its behaviour in living bodies when the administrated dose was low.
In this paper, some information was obtained at a low concentration of administration---information on absorption, excretion, and accumulation of methyl mercury in living bodies using mice of d.d strain, transformation into fetus using rats of Wisterstrain.
Results are as follows;
1) Absorption rates from the digestive tract of orally administrated mercury compounds were Methyl mercury choloride (MMC) 73%, Phenyl mercury acetate 45% and Mercury acetate 6% after about 6hrs.
2) Accumulation in the bodies of mice given solid foods containing MMC 1.0ppm (mercury equivalent) showed apparent increase. But experiments revealed that there was a certain limiting value in accumulation after about 75 days, the value being about 40μg.
3) 23.2% of the total administrated MMC dose taken into the mother body passed into the fetus group. MMC concentrations in brain and liver were higher in the fetus-body than in those of the mothers.
4) Contents of mercury in blood and hair apparently showed lower values in pregnant rats than in the non-pregnant.
5) In comparison with human mother and their new-borns, mercury content in blood corpuscles was higher and that of serum was lower in the new-born.

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