In February 1962, in Miyazu city of Kyoto Prefecture, a food poisoning event occurred which closely resembled globefish poisoning. According to Dr. Hobo's epidemiological investigation a conclusion was reached that the food directly connected with the poisoning was the oyster which had been cultivated in Miyazu Bay.
An experiment was conducted to clarify the cause of this poisoning and the following results were obtained.
1) The poisonous component of the oysters was soluble in water.
2) This component was found in abundance in the internal organs, and the potency was lost by boiling in alkali.
3) The purified poison which was extracted with methanol and a purified method performed with ion exchange resine, charcoal and cellite had lethal doses of aproximately 1.2μg for 20g mouse parentally administered.
4) The poison was slightly different from the paralytic shellfish poison of mussel and Akasaragai as well as that of the globefish.

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Since 1961, the area of Fukuoka City as well as other areas of Japan has been polluted by radioactive fallout brought about mainly by the fission and fusion bomb test in Red China. In this area periodic measurements of radioactive fallout deposits were measured on the Standard Diet proposed by the Department of Welfare in Japan. In addition measurements were taken on several kind of vegetables (well washed), dairies, row milk, short necked clam and the Tapes Philippiarium. Results obtained from these surveys are as follows:
1) The level of radioactivity of the fallout deposited on the vegetables resulting from the 1st, 2nd and 5th tests of the atmic bomb in Red China increased remarkably from 10 times to 200. This is much higher than that of the usual amount of fallout on vegetables. Especially noted was that a somewhat higher level of radioactivity than normal level was maintained for several months during the time of the 5th test.
2) Increase and decrease of radioactivity on seasonal vegetables fluctuated approximately with those of the radioactive fallout deposited on the vegetables. The level of radioactivity on the vegetables increased sharply from normal level measured on them after the test.
3) Although radioactivity level on the vegetables had increased from the usual level, the level measured on dairy and row milk did not fluctuate remarkably during the test.
4) The degree of radioactivity in the short necked clam with shell removed fluctuated considerably more than the usual level, eg. 0.5 to 38.9. However there was no evidence of increase of radioactivity in the clam after the test.
5) The degree of radioactivity in the Standard Diet in Japan increased slightly in 1967 compared to that of former years.
6) Giant particles of radioactive fallout precipitated on spinach just after the fifth test of the Atomic Bomb in Red China were discovered by means of autoradiography. Removal of these particles could not be completely successfully accomplished with a soapless soap.

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Experimenting with steady exposure numerous studies were made on the physiologically induced responses of exposure to noise. The result concluded was that the central location of physiological damage by noise quite possibly is in a higher centre.
Next the metabolic change under noisy environmental conditions was investigated. It was noticeable that the ammonia content in the brain was significantly elevated in the case of noise exposure. This fact could not be observed in other stress exposures. It was brought about by the disturbonce in the removal process of the ammonia not by the acceleration of the ammonia production.
In this study, white noise was used at 80 or 90dB (C) from 2 to 4 hours. Intermittent exposure was made under 2 conditions, i.e., one the condition that repeated a cycle formed by ON-time of 30 seconds and OFF-time of 30 seconds (ON-OFF 30sec.), another the condition that repeated a cycle formed by ON-time of 300 seconds and OFF-time of 300 seconds (ON-OFF 300sec.). The ON/OFF ratioes were 1 in both intermittent exposures.
Adult male rats were exposed to noise, then were killed by decapitation. The brain was rapidly removed, and homogenized in ice-cold 10% trichloroacetic acid with glass homogenizers. The estimation of ammonia was made by Conway's method using Seligson-Hirahata's apparatus.
The following results were obtained:
At 80dB exposure, the central ammonia concentration was elevated significantly under steady and ON-OFF 300sec. intermittent exposure for 2 and 4 hours, but not under ON-OFF 30sec. intermittent exposure for 2 and 4 hours.
Under ON-OFF 30sec. intermittent exposure to 90dB, the central ammonia content was elevated significantly during a 4 hour period but not during a 2 hour period.
From the above mentioned results, the following concepts may be considered;
1) Injury by steady exposure to noise will be higher than that caused by the intermittent exposure.
2) From the view-point of biological damage, intermittent exposure may be characterized by the following 2 factors;
a: duration of one duty cycle
b: total ON-time during the exposed period.

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Based on the recent reports that the changes of the cerebral ultrasonic attenuation (CUSA) can be an indicator of the cerebral circulation under certain conditions, the following two experiments were carried out to clarify the relationship between nervous activities and the cerebral circulation. The examinees were healthy male students, 22 in experiment 1 and 15 in experiment 2.
1. Changes of CUSA were observed under exposure to sonic boom and gradually increasing noise. The latter caused no change of CUSA, while the sonic boom resulted in a characteristic pattern change of CUSA with an initial increase and its early restoration to the original state.
From these observations it is assumed that a strong sensory stimulation caused a temporary decrease of the cerebral circulation. This is restored quickly by autoregulation of the cerebral circulation and the movement in body circulation.
2. The change of CUSA due to postural change correlated significantly with the CPT-swing degree.
These observations reveal that the homeostasis in the circulatory system appears to be parallel for both tests.

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For the purpose of physiological analysis of the function of concentration maintenance, an experiment on the changes of the cerebral circulation in nervous activities was carried out with the measurement of the changes of CUSA and the recording of the brain waves in the TAF test. The examinees were nineteen healthy male students.
The results obtained were as follows:
1) CUSA decreased during the TAF-test and, by stopping the test returned to the initial level.
2) These changes correlated with the changes of the alpha-wave of the brain waves, that is, the stronger the alpha-blocking during the TAF-test, the greater the changes of DUSA.
3) This excellent group, in a ten-minute TAF-test showed a tendency of a greater change of CUSA than that of the inferior group in TAF-test.
These results, together with the present knowledge on CUSA and cerebral circulation, lead us to the conclusion that the use of the TAF-test resulted in the rise of the brain activity lelel around the cerebral cortex and the brain-stem reticular formation, which in turn brought about an increase in cerebral circulation in parallel with the changes of the brain waves. Also when testing this excellent group in comparison to the inferior group, it is considered that the TAF-test for the relatively long duration of ten minutes as in this experiment required a more strenuous effort in concentration of attention, consequently, the grade of increase in the cerebral circulation was greater in the excellent group.

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Six kinds of arm covers which contact tightly around the joint regions of the elbow were prepared, and the degree of deformation from each cover and the restrictive pressure on the skin ot the joint were measured and their results were compared with the restrictive psychological feeling, when the arm was strongly bent.
(1) It was found that the deformation and vertical (i. e. restrictive) pressure on the skin were exceedingly greater on the outer part of the ioint, and that the both values were larger in the case of the cover cloths which are stretchable in the longitudinal direction of the arm rather than the case of that in the round direction of the ebow.
(2) Functional tests were applied after Sheffés method of “paired comparison”. Exactness of judgment of the wearers was tested by the “testing of coefficient of consistence” and then the degree of judgment between the wearers was tested by the “testing of coefficient of agreement”. A high correlation was found in each case. Numerical values were obtained by the analysis of variance from the results of judgment.
(3) It was further noted that no linear relation exists between the deformation and the value of psychological feeling and a distinct linear relationship does exist between vertical pressure and the value of psychological feeling.

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As previously reported, ⁹⁵Zr-⁹⁵Nb were detected in fallout and also in vegetation in the Fukuoka Prefecture even at other times besides occasions of nuclear explosion. It is suggested that an intake of a considerable dose of these radioactive agents may occur in common daily dietary food. In succession of Report I, which described the distribution and excretion of these radioactive agents in subcutaneously injected rats, an oral administration in rats using intubation technique was carried out consecutively and the distribution and excretion of these radioactive agents were pursued by using a G. M.-tube counter.
1) Radioactivities of every observed organ other than the stomach revealed the maximum value at the 24th hour after the administration, rapidly reduced up to the 7th day and very slowly decreasing.
2) A remarkable deposition of ⁹⁵Zr-⁹⁵Nb in tibia as distinguished from other organs was not observed in early stages after the oral administration although it differed from the former subcutaneous injection. But on the 63th day after administration, an amount of deposition of ⁹⁵Zr-⁹⁵Nb in second place after the spleen, was detected in the tibia.
3) The amount of 66.22% of the total excretion of ⁹⁵Zr-⁹⁵Nb was excreted by the 7th day after administration and also was measured in feces more than in urine. From the fact mentioned above, it is considered that ⁹⁵Zr-⁹⁵Nb given by oral administration to rats may have poor absorption by the alimentary tract.

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