A cell line was newly established from a lymph node with metastasis from oat cell carcinoma of the lung.The culture medium consisted of RPMI-1640 supplemented with 20% fetal bovine serum. The culture cells grewfloating free in the culture fluid and have been serially subcultured for 80 generations, up to date. Doubling timewas approximately 24 hours. Multi-lobulated nuclei and well-developed Golgi apparatus were prominent and secretorygranules were occasionally observed. No virus particles were found. Repeated examination ofimmunofluorescence revealed no evidence of immunoglobulin synthesis and EB virus production. Chromosomalanalysis of cultured cells of the 8th generation revealed the modal number of 47 chromosomes and the karyotypicanomaly of B5q+, t (Alq+; Cq-)- in all of the mitotic cells.
These characteristics of this cell line obviously differ from the lymphoblastoid cell lines derived fromthe human lymphoid tissues, strongly supporting the possibility of its cancer cell origin. This cell line, hence, wasdesignated as OAT line after oat cell carcinoma.

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Search for possible tumor-specific reaction in human lung cancer was attempted by the indirect membraneimmunofluorescence technique. Living cells of the established cell line, OAT, derived from human pulmonarycarcinoma of oat cell type, were used as the target cells and both FITC-conjugated anti-human IgG and Ig Mgoat sera were used as the second reagents, respectively. Five out of 8 sera from the patients with pulmonarycarcinoma of small cell type gave positive membrane immunofluorescence reaction with anti-Ig M reagent but notwith anti-IgG. However, 27 sera from patients with other types of carcinomas, including 9 cases of adenocarcinomaof the lung, and 8 sera from healthy individuals gave no positive reaction. No membrane immunofluorescencereaction was given by the selected positive sera, when it was tested against the cell line of Burkitt lymphoma origin, P3HR-1. The results strongly suggest that the antibody against the cell surface of OAT cells resides in IgM fractionof sera from the patients with small cell carcinoma and that the antigen specific for and common to small cellcarcinomas of the lung.

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Two cases of Chondrosarcoma of rib origin are presented.
Case 1: A 17-year-old male, incident to a school routine chest roentgenogram, was found tohave a abnormal shadow of the left chest field. At first, he was treated as pleurisy at anotherclinic, later referred to our clinic for operation, under the diagnosis of infiltrated carcinoma followingopen biopsy for the left chest wall tumor.
A block resection of the mass was carried out with removal of the second to fouth ribs involved. The histological examination of the specimen showed chondrosarcoma of rib.Case 2: A 46-year-old man, first entered our clinic, with a compliant of a painful mass onthe upper right part of the chest with diagnosis of tuberculous abscess of rib and antituberculousadministration for several months. Ten months after the patient was readmitted to the clinic, becauseof gradual increase of the mass in size.
The mass togethere with the fourth, fifth, sixth ribs had been resected.
Diagnosis was chondrosarcoma of rib.
Both of the patient's postoperative course were uneventful.
A brief reveiw was made on 11 cases, 9 cases collected from the literature, 2 from our ownrecords.

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