遺伝学雑誌
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
33 巻, 6 号
選択された号の論文の4件中1~4を表示しています
  • Yasushi TAKENOUCHI
    1958 年 33 巻 6 号 p. 163-175
    発行日: 1958年
    公開日: 2007/11/30
    ジャーナル フリー
    The chromosomes of thirty species of Japanese weevils (Curculionidae) belonging to twelve subfamilies were studied in germ cells. Data on the species investigated and their chromosome numbers together with the sex-determining mechanism are summarized in Table 1. The number of chromosomes shows a variation ranging from 11 to 23. The species showing the haploid number of 11 are numerous being 36.8per cent in frequency. Generally most species of the same genus have a similar chromosome number. All the species under the study reported in this paper are characterized by an X-Y sex-determining mechanism in males.
  • 岡田 利彦
    1958 年 33 巻 6 号 p. 176-187
    発行日: 1958年
    公開日: 2007/11/30
    ジャーナル フリー
    When the mixture of E. coli B and T3 phage are plated using the double agar layer method described by Adams, the T3 phage-resistant bacteria form the colonies on and in the upper layer after incubation of 24hrs, at 37°C. Among the resistant colonies formed, the chewed up sectored colonies and the so-called thin colonies were frequently observed. These colonies have been reported by Demerec and Fano (1954) and by Fraser (1954). However, the mechanism of the sectored colonies formation have not been studied precisely. The present paper concerns the experiments which were undertaken to reveal the mechanism.
    The sectored colonies (i.e., the bacteria and the phages lysing them) could be maintained by the transferring the colonies on the neutral agar slant medium. Hence, in the experiments these colonies transferred several times on the slant agar were used.
    The bacteria obtained from the sectored colonies were B/3, 4, 7 and the phages lysing the colonies T3h mutants. The former were designated as Bs or Bs/3 and the latter T3s or T3hs for convenience, because the bacteria and the phages seemed to be specific strains.
    When the Bs/3 strains were mixed with T3h phage in the appropriate ratio and plated by the the double agar layer method, the sectored and thin colonies could be reconstructed. Then, it was concluded that the sectored and thin colonies were formed by the interaction between B/3 and T3h phage: during the course of the B/3 colonies on the plate, they were lysed by T3h mutants which appeared among the great number of T3 wild type phages grown on E. coli B wild type strain.
    Furthermore, the sectored colonies were also obtained by mixing E. coli E wild type strain with T3 wild type phage or T3hs phage. When the sectored colonies reconstructed by mixing E. coli B and T3 phage, were incubated at 37°C for 48hrs., the papillae-like secondary colonies appeared on the thin colonies. However, when the T3hs phage instead of the T3 wild type phage was used, the secondary colonies were not observed. Therefore, the colonies would be T3 phage resistant colonies which appeared in the thin colonies.
  • 岡田 利彦
    1958 年 33 巻 6 号 p. 188-191
    発行日: 1958年
    公開日: 2007/11/30
    ジャーナル フリー
    以上を要約すると次のようになる。T3ファージ抵抗性菌の集落の中に見出される扇形集落を斜面培地で何代か継代すると, はじめはB株上で円形の溶菌斑を形成する T3hファージが, 同じB株上に不整形の溶菌斑を形成するファージ集団に置きかえられているのを見出した。これを T3hs と名付けたが, このファージの Bs/3 株で増殖したもの [T3hs (Bs/3)] は, B株上に不整形のBs/3株上に円形の溶菌斑を形成し, B株で増殖したもの [T3hs (B)] は, B株上にも Bs/3 株上にも円形の溶菌斑を形成した。
  • I. 各発生時期におけるパラチオン抵抗性
    田村 真二
    1958 年 33 巻 6 号 p. 192-199
    発行日: 1958年
    公開日: 2007/11/30
    ジャーナル フリー
    1. キイロショウジョウバエ(D. melanogaster)の殺虫剤抵抗性系統 Hikone および WMB と非抵抗性系統 Fukuokaおよび Canton-S のそれぞれ1令 (孵化直後), 2令 (孵化後約35時間), 3令成熟幼虫 (孵化後約96時間) をパラチオンの各種濃度含有の飼料で飼育し, その蛹化率, 羽化率等に対するパラチオンの影響をしらべた。
    2. Fukuoka, Canton-S, 両系統の1令幼虫は0.1ppmパラチオンで蛹化率, 羽化率ともにそれぞれ対照の4%, 1%に低下したのに対し, Hikone, WMB 両系統の1令幼虫は3.0ppmパラチオンにおいても蛹化率はそれぞれ対照の35%, 26%, 羽化率はそれぞれ対照の34%, 26%に低下したのみであった。
    3. 2令幼虫の場合は Fukuoka, Canton-S 両系では0.1ppmのパラチオンですでに蛹化, 羽化するものがまったくなかったが, Hikone, WMB 両系統では3.0ppmのパラチオンにおいても蛹化率はそれぞれ対照の27%, 14%, 羽化率はそれぞれ対照の26%, 14%を示した。
    4. 3令成熟幼虫においては Fukuoka, Canton-S 両系統でも1令, 2令幼虫に比して相当の抵抗性を示し, 50.0ppmのパラチオンにおいてもそれぞれ対照に比して21%, 39%の蛹化率を示し, Hikone, WMB 両系統では50.0ppmのパラチオンでそれぞれ対照に比して21%, 19%の蛹化率を示したが, 羽化率においては前2者は 50.0ppmで羽化するものはなかったのに対し, 後2者はともに対照に比して3%の羽化率を示した。
    5. 抵抗性, 非抵抗性の系統における幼虫期間, 蛹期間の長さには有意義な差異は認められなかったが, 抵抗性の1令, 2令の場合, 非抵抗性のものに比べて幼虫期間の延長の傾向が認められた。
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