遺伝学雑誌
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
65 巻, 1 号
選択された号の論文の4件中1~4を表示しています
REGULAR PAPERS
  • Wataru SAKAMOTO, Koh-ichi KADOWAKI, Shigemitsu TANO, Tomosaburo YABUNO
    1990 年 65 巻 1 号 p. 1-6
    発行日: 1990年
    公開日: 2006/06/10
    ジャーナル フリー
    It was described previously that the combination of the cytoplasm of a Japanese variety of Oryza sativa and the nucleus of O. glaberrima produced by successive crossings led to male sterility. In the present study, the mitochondrial DNAs (mtDNAs) were isolated from this cytoplasmic male-sterile (CMS) strain and its maintainer strain, and were analyzed with restriction enzymes and by agarose gel electrophoresis. Differences in the restriction pattern were observed between the CMS strain having sativa cytoplasm and its maintainer strain having glaberrima cytoplasm. The plasmid-like DNAs (B1 and B2), which are often observed in mitochondria of CMS strains derived from O. sativa, could not be detected by Southern hybridization analysis as well as gel electrophoresis. It was suggested that the plasmid-like DNAs were not associated with the cytoplasmic male sterility. However, the homologous sequences to B1 and B2 were detected in the nuclear genomes of all species tested.
  • Yutaka OONO, Kiyoshi KANAYA, Hirofumi UCHIMIYA
    1990 年 65 巻 1 号 p. 7-16
    発行日: 1990年
    公開日: 2006/06/10
    ジャーナル フリー
    Progeny plants obtained by self-crossing transgenic tobacco plants possessing the rolC gene (ORF 12 of TL-DNA of Ri plasmid) showed stable inheritance of dwarfness in plant height as well as in floral organs. A histological analysis of dwarf plants indicated that shortening in the internodal length was associated with a smaller length in epidermal cells. Furthermore, transgenic plants expressing dwarf characters showed earlier flowering than normal one. The organ-specific transcription of the rolC gene was independent of the influence of other Ri TL-DNA genes.
  • Hirokazu HANDA, Yasunobu OHKAWA, Kousuke NAKAJIMA
    1990 年 65 巻 1 号 p. 17-24
    発行日: 1990年
    公開日: 2006/06/10
    ジャーナル フリー
    Restriction endonuclease analysis was carried out for mitochondrial (mt) DNAs of 27 accessions of rapeseed (Brassica napus L.) to detect intraspecific variation of the mitochondrial genome. MtDNA isolated from green leaves was digested with ten endonucleases and was electrophoresed using agarose slab gel. Mt genomes of the 27 accessions were classified into two major types, each of which was further divided into three subtypes based on the length differences in the restriction fragments of mtDNAs. In total, six mt genome types (type Ia, Ib, Ic, IIa, IIb and IIc) could be identified. To analyze the relationship between the mt genome types and the cytoplasmic male sterility (cms) induction, restriction fragment patterns of two male sterile cytoplasms, namely, nap cms and pol cms, were compared with those of the normal cytoplasm. The mt genome of the nap cytoplasm was found to be either of Ia, Ib and Ic types, whereas the pol cytoplasm carried the type IIb mt genome. Mitochondrial linear plasmid DNA was also observed in several accessions, but the relationship between its presence and the cms trait could not be established.
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