Three-day-old etiolated rice seedlings were treated anaerobically for 24hours. Respiratory change of the shoot following transfer from anaerobic to aerobic condition was studied.
1. Treated samples showed a considerably high level of respiration (Qo
2) at 30min. after return to air, followed by a further rise to reach in 3 hours of airadaptation a maximum plateau, which was maintained at least for further 4hours. It was ascertained that this rise in respiration was not due to microbial contamination.
2. One-hour's aerobic period inserted into a period of anaerobic treatment increased the respiratory activity as measured after the whole treatment. About 2-hours' contact with air was, however, necessary for the completion of the respiratory rise.
3. By cooling at 0°at the transference into air, the beginning of the respiratory rise was delayed just by the length of the cooling period.
4. The 24-hour anaerobiosis in the 3-4 day period stimulated coleoptile elongation by about 20%, while it inhibited fresh weight increase of the shoot.
5. When treated shoot sections were transferred to air, rapid water uptake took place before the maximal respiration rate was achieved, and little or no fresh weight increase occurred in the remaining period, while in the aerobic control fresh weight increased at a constant rate during the corresponding period.
6. Respiratory activity per shoot did not change during the anaerobic treatment while it increased in the aerobic control during the corresponding period.
Following transference to air the respiratory activity remarkably increased to catch up with the level of the control in 24hours.
7. Total protein content of the shoot responded to oxygen less remarkably than respiratory activity. The latter seems to be under a regulatory mechanism
more sensitive to oxygen than are some other cellular metabolisms.
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