I. During one month investigations were carried out for changes of the degrees of light-sensitivity (represented by germination) of tobacco seeds with the lapse of time of imbibition and the following results were obtained.
(1) In the case of “Daruma”. “Oo-Daruma”. “Hatano”. “Suifu” and “ Kokubu”. increases and decreases of their light sensitivity form repeating waves of 2 to 4 times with the lapse of
time of imbibition (presoaking time). According to the degree of after-ripening of the tested seeds and the strains of them, there exist some differences in imbibiting time which both maximum and minimum values of light-sensitivity can be obtained. In the case of seeds after-ripened for 5 months, the largest light-sensitivity are obtained on the 3rd or 4th, 8th and 15th days of soaking, and completely after-ripened seeds show the largest increases of light-sensitivity on the 3rd, 5th and 10th days.
(2) However, light-sensitivity of the seeds of “Yellow” alone do not show repeating wave-like changes but form a gentle ascending and descending slope with the lapse of time of imbibition. This way of changing is the same as in seeds of certain other plants which has been observed by some investigators up to the present. The peak is on the 8th day of soaking, in freshly collected seeds, and 4th or 5th day, in completely after-ripened ones.
(3) Differences between higher points and lower points of the waves described in (1) set forth respectively different features according to the strain. “Daruma” exhibits the largest differences which is followed by “Suifu”. then “Hatano”. and “Kokubu”. that is to say, the last waves have the least uneveness. “Yellow” forms only one wave of a mountain shape as described in (2).
(4) If seeds were exposed to a large quantity of light such as 10
4 MK-3min. (1, 800, 000 MKS), changes of light-sensitivity exhibit a simple form like “_??_” and no wavelike curves can be obtained.
II. One of the experiments of the present work in which seeds are given a very brief exposure to a very small quantity of light proved that “Yellow” is extremely light-sensitive. Because despite the fact that the values of the time and the light quantity of exposure were extraordinary small, however a considerable germination percentage could be obtained. Such a small figure was never experienced before.
(1) In the case of fully ripened seeds of “Yellow”. 75% of germination can be obtained by 1, 500 lux (MK)×one-ninetieth second and 54%, by 150 lux×one-ninetieth second, after 72hrs, of soaking, in other words, it can be obtained by a very brief exposure to Light in both cases, (The maximum percentage of germination of this strain of tobacco is 80 to 85)
(2) The seeds of “Yellow” which do not reach full-ripening showed 71.5%, 62.5% and 35.5% of germination by 1, 500 lux×one-ninetieth seconed, 200 lux×one-hundredth second and 200 lux ×one-threehundredth second respectively on the 8th day of soaking which is the peak day of light-sensitivity. (The maximum germination percentage of this strain is 70.)
Thus, even by an extraordinary short exposure to light, we can secure, in this kind, about half figures of the maximum germination percentage. In conclusion, the result showed that a very small dosage of Light promotes germination.
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