Sugars condained in the alcohol extracts of seed embryos of
Vigna sesquipedalis germinated in the dark were studied semi-quantitatively on onedimentional ascending paper-chromatograms.
No other sugar than sucrose, glucose and fructose was detectable, excepting that two unidentified blue-colored spots of relatively high Rf values were made appeared when resorcinol-HCl was sprayed. These sugar-like substances behaved different on paper-chromatogram from galactose, mannose, rhamnose, xylose, arabinose and ribose; they also did not react with the diphenylamine as well as the orcinol reagents.
The seeds before germination contained only sucrose, no hexose being detected. As germination proceeded, glucose and fructose were formed rapidly to predominate soon over sucrose in the epicotyl, hypocotyl and radicle. In plumules, on the contrary, the amount of glucose and fructose did not exceed that of sucrose; the production of fructose in particular was very much small. The cotyledon, as a homologous organ to the plumule, showed a similar sugar pattern to that of plumules, i.e. a bulk of sucrose accompanied by only a scanty amount of glucose and no fructose.
In the cotyledon tissues maltose was absent, and, therefore, the starch degradation might not be performed by the action of amylase but likely by the phosphorylase action instead.
These results would suggest, in accordance with Wanner (1952), that in the cotyledon sucrose may be synthesized from the products of starch degradation, hexoses, and transported as such into the growing parts of the germs to be split again to free hexoses probably by the action of invertase.
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