Two endogenous rhythms, light-on and light-off rhythms, are associated with the photoperiodic floral response of
Pharbitis nil1, 2). The light conditions required to initiate or reset these rhythms were investigated.
Two hours of light at 4000lux was not enough to initiate its own rhythm when applied 8hours after the beginning of the dark period; i. e. the light-off rhythm initiated at the beginning of the preliminary 8-hour dark period still persisted after the 2-hour light period. Light periods of 4 to 8hours were sufficient to initiate a new rhythm even at 10-70lux.
The light-off rhythm was initiated when plants were transferred from 4000lux to 10lux and proceeded normally under 10lux. Transfer of plants from 4000lux to 250lux did not initiate the light-off rhythm, but transfer from 250lux to darkness was effective.
Spectral dependence of light required to initiate the light-off and/or light-on rhythms was very similar to that for light break and the reversion of the far-red effect. Blue, green, red, and white light of 1000, 30, 12, and 30μw/cm
2, respectively, were equally effective for induction of the light-off and/or light-on rhythms.
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