遺伝学雑誌
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
42 巻, 5 号
選択された号の論文の9件中1~9を表示しています
  • FUSAO MOTOYOSHI
    1967 年 42 巻 5 号 p. 291-297
    発行日: 1967年
    公開日: 2007/05/21
    ジャーナル フリー
    Four chlorophyll mutant strains of sand oats (albino-R5, -R6, R7 and yellow-1) and two of barley (yellow chlorina and yellow green) were cultured in test tubes to investigate their nutritional requirements for chlorophyll formation. Plants were cultured in test tubes containing nutrient agar media. Effects of glucose, casein hydrolysate, and yeast extract on chlorophyll formation were examined using leaves of these mutants. Glucose was effective on albino-R5 and yellow-1 mutants of oats to increase chlorophyll content, while casein hydrolysate and yeast extract were ineffective. In albino-R6 and -R7 no increase of chlorophyll was observed in the media used. In yellow chlorina and yellow green mutants of barley, the addition of glucose caused an increase of chlorophyll content. Only yellow green mutant responded to casein hydrolysate, whose increasing concentrations resulted in a higher content of chlorophyll. Alanine, valine, leucine and glutamic acid also stimulated chlorophyll formation in this mutant.
  • T. KANEHISA, S. FUJII
    1967 年 42 巻 5 号 p. 299-306
    発行日: 1967年
    公開日: 2007/05/21
    ジャーナル フリー
    Protein fractions of the Drosophila larva in tu and Oregon R strains by means of an ammonium sulfate were studied by Tiselius-electrophoresis and by ultraviolet analysis, and it was found that a qualitative difference between tu and the control was electro-phoretically observed in a fraction (F-3) of five fractions obtained from the 12000g.- supernatant of larva-homogenates treated with an ammonium sulfate (2.8M in final concentration); namely, the tu material has only one peak in a direction “up” although the control shows two little peaks. A quantitative difference of ultraviolet-absorption at around 285mμ was also observed between tu and the control in a fraction (F-2).
    On the other hand, tumor-inducibility of “Freckled-type” melanotic tumor, F-type, was detected by injection of three fractions (F-1, F-2, and F-3) separated from these larva-homogenates by DEAE-cellulose column-chromatography (NaCl-linear concentration gradient-elution), into both pre-third instar larva of Varese (non-tumor strain) and Chieti-v (a strain with low tumor incidence of ordinal melanotic tumor, M-type). The results showed that the inducibility of F-type tumor in Varese and the relatively higher inducibility of M-type tumor might be in the F-2. Further, the F-2 was checked for the existence of nucleic acids by ultraviolet analysis, orcinol and diphenylamine reactions, and 32Pi-incorporation experiment. However, negative results were obtained. From these experimental results and the previous reports (Kanehisa 1960b), it is suggested that the F-type tumor-inducible factor (or factors) may not be a substance with nucleic acids but a kind of biochemically active protein which contains tryptophan or its derivative, and that such a specific factor may not specifically be different from a factor for M-type tumor and its specificity for F-type tumor may depend on hereditary nature of the host-itself responsible for the factor.
  • HACHIRO SHIMBA, SAJIRO MAKINO, MOTOMICHI SASAKI, MICHIHIRO C. YOSHIDA, ...
    1967 年 42 巻 5 号 p. 307-315
    発行日: 1967年
    公開日: 2007/05/21
    ジャーナル フリー
    Thirty-two patients associated with sex abnormalities were screened for chromosome abnormalities based on short-term leucocyte cultures. Among them, 12 cases were found to be chromosomally abnormal. They are 1 case of suspected Klinefelter's syndrome with an XY/XXY mosaicism, 6 cases of Klinefelter's syndrome with a regular XXY sex-determining mechanism, 2 cases of Klinefelter's syndrome with an XXYY sex-mechanism, 1 case having small penis with an XY/XXY mosaicism, 1 case of cryptorchism with an unusually long Y, and 1 case of azoospermia showing a relatively high incidence of chromosome breakage. Out of the 6 regular Klinefelter cases, one had a long Y chromosome and two showed chromosome breakage at a relatively high incidence. The chromosomes of the remaining 29 cases did not deviate from a normal complement, being 46, XX in females and 46, XY in males.
  • SUMIO MINAMORI
    1967 年 42 巻 5 号 p. 317-326
    発行日: 1967年
    公開日: 2007/05/21
    ジャーナル フリー
    Some flies having a killing agent were found in sampled flies of Drosophila melanogastey from a natural population in 1964. The number of their offspring was reduced distinctly, and the distorted segregation of second chromosomes in the offspring was observed by using dominant marker genes.
    The killing agent was transmitted from both females and males to their progenies. The agent had been maintained by a carrier strain, though its all original chromosomes were substituted with marked chromosomes. However, most strains, having the killing agent originally, had lost it within a year. The killing agent seemed to have been transmitted by copulation from males to females and by contact of larvae. The rate of development or multiplication of the agent at 30°C was faster than that at 20°C. Flies under two days old could not transmit the agent to their progenies. Such experimental results indicated that the killing agent might be an infective microorganism for flies.
    The survival rates of flies carrying Cy, L, Pm or bwD chromosomes respectively to the killing agent were observed to be different. The similar experiment was performed by using 184 second chromosomes isolated from a natural population. The survival rate of flies carrying chromosomes derived from the carrier strain was higher than that of flies having no infection. Flies carrying a lethal chromosome reduced the survival rates more than flies carrying a quasinormal chromosome.
  • YUKIKO KATAOKA
    1967 年 42 巻 5 号 p. 327-337
    発行日: 1967年
    公開日: 2007/05/21
    ジャーナル フリー
    A system of suppressor for the SD action in a natural population of D. melanogaster in Odate, Japan was analyzed. Most of the suppressors were located on the X chromosome. Two kinds of second chromosomes were found in the same population, one of which was a chromosome carrying insensitive element to the SD action and the other which inhibits the effect of suppressor. The effect of the suppressor was different for the SD which originated from different populations. The suppressor was located somewhere between the v and f loci on the X chromosome. The suppressor-X chromosomes were found to show slight but consistent meiotic drive, and in relation to this effect, a genetic mechanism regulating sex ratio in the population was discussed.
  • XVI. KARYOLOGICAL STUDIES ON SENSITIVE AND RESISTANT SUBLINES OF THE MOUSE LYMPHOCYTIC LEUKEMIA, L-1210, TO SEVERAL ANTITUMOR AGENTS
    TOSIHIDE H. YOSIDA, KYOKO OHARA, LLOYD W. LAW
    1967 年 42 巻 5 号 p. 339-347
    発行日: 1967年
    公開日: 2007/05/21
    ジャーナル フリー
    Chromosomes in a drug sensitive and 5 drug resistant sublines of mouse leukemia L-1210 were studied. The 5 sublines used in the present study were resistant to methotrexate (L-1210/AM), 6-mercaptopurine and fluorouracil (L-1210/6MP-FU), 6-mercaptopurine, fluorouracil and methotrexate (L-1210/6MP-FU-AM), antileukemic agent NSC-38280 (L-1210/38280) and thioguanine (L-1210/TG). The chromosome numbers in sensitive L-1210/s cells varied from 34 to 46 with the mode at 40. Resistant sublines L-1210/AM, L-1210/6MP-FU, L-1210/6MP-FU-AM and L-1210/38280 also had 40 chromosomes as the mode of variation, fluctuating from 36 to 45. The L-1210/TG subline had only 41 chromosomes as a mode. A large submetacentric marker chromosome was usually found to characterize the sensitive line. In none of resistant lines, however, this marker chromosome was observed, while a small minute telocentric or metacentric and long telocentric T-markers were often observed in them. The relationship of these changes in karyotypes and type to resistance was discussed.
  • YASUSHI TAKENOUCHI
    1967 年 42 巻 5 号 p. 349-350
    発行日: 1967年
    公開日: 2007/05/21
    ジャーナル フリー
  • YASUSHI TAKENOUCHI
    1967 年 42 巻 5 号 p. 351-352
    発行日: 1967年
    公開日: 2007/05/21
    ジャーナル フリー
  • 太田 朋子
    1967 年 42 巻 5 号 p. 353-360
    発行日: 1967年
    公開日: 2007/05/21
    ジャーナル フリー
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