Calcium and radiostrontium-contents in food-materials produced during 1955 to 57 were determined.
In many kinds of food-materials produced on land such as cereals, vegetables, and milk etc., Sr⁹⁰-content was found to attain more than20Sr U, while, in those in marine as seaweeds and dry fishes, this was so small that it was barely recognized.

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The determination of choline content in cereals and seeds commonly used in Japan was made from the standpoint of food chemistry. The determination was carried out by the Reineckate method with methanol extraction. The results were as follows:
1) The contents for rice and its bran were 0.4 to 0.8 and 1.0 to 3.2mg per gram, respectively. The choline loss in rice while polishing ranged from 6 to 43%.
2) The choline content in the bran was found to be the largest in that obtained when rice was polished to 94% of its weight of others obtained when polished rice mor or less than 94%, and, from this, it seems probable that in the bran layer this is deminantly localized especially in the outer endosperm and aleurone layer.
3) Wheat, barley, and oat contained about 1.0mg choline per gram, and rye 0.7mg.
4) Corn grown in cold region contained more choline than that in the warm one, and it seems probable that the biosynthesis of choline is affected by temperature.
5) Among the seeds examined, it is also found that the high fat seeds have a tendency to contain larger amount of choline than low ones.

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From the dietary survey of 50 families of Hachijo Island's inhabitants, it is observed that the diets of these are greatly unbalanced on its nutrients composition, the comsumption of foods of animal source, vegetables, and fruits was low, and so the intake of animal protein, inorganic substances, and vitamins was found to be difinitely low.

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From the physical inspection with 263 residents of Hachijo Island, it would be concluded that the incident ratio of Anemia, Hyperkeratosis, Galt Tenderness, Angular Stomatitis, Edema was almost the same as that obtained with the other districts of Tokyo, while some showed abnormal rate of pulses. It was also observed that minimum blood pressure was lower than that of normal one and the incidence of missed and decayed teeth was abnormally high.

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Blood samples were collected from 76 Hachijo's inhabitants and the specific gravity and the contents of its inorganic and organic components, namlly, total serum calcium, inorganic phosphate, serum protein, albumin, and globulin were compared with those of inhabitants dwelling in nutritively improved area (area of Suginami.Nishi Health Centre, Tokyo) and it was found that total calcium and dialyzable calcium of Hachijo's inhabitants were definitely low.

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The decomposition ratio of dehydro-l-ascorbic acid (DAA) increased with the increase of the duration of its boiling at every pH (1.0-8.2), and the decomposition of it was especially remarkable in the case of its boiling for 10 minutes at pH 6.4-8.2.
Thiourea, NaCl, and thiamine prevented the decomposition of DAA in pure solution during boiling, while could not this in cabbage.Cane sugar and pyridoxine had no perceptible effect upon the decomposition, while the solution of egg albumen and cystine accelerated it.

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In continuation of the work reported previously1), the extention of storage period of steamed fish paste products by Co-60 gamma irradiation was studied at higher storage temperatures. When vacuum packed baked samples were irradiated, a considerable in crease in storage period was observed. However, the loss of the originalflavor after irradiation and a change in color on storing occurred, these changes being marked with a large irradiation dose. An early stage of deterioration was detected by turbidmetry, which has been suggested by Tomiyama, et al 4), along with organoleptic observations.

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Albino rats weighing 40 to 60 grams were maintained on the following diets: 1, low lysine basal diet containing corn meal 76, wheat gluten 20, sodium chloride 1, calcium carbonate 3, and a vitamin mixture; 2, basal diet plus D-lysine hydrochloride 0.5%; 3, “plus L-lysine hydrochloride 0.5%. 4, ” plus DL-lysine hydrochloride 1.0%. The samples of lysine used here were synthetic products derived from r-caprolactam.
The addition of L-lysine stimulated the growth, and of DL-lysine in somewhat less extent. Some of the animals in the D-lysine group were dead after sudeen less of weight, but it could not be attributed to the deleterious effect of the D-compound.
Feeding of DL-lysine over long period caused slight pathological changes in liver and kidney.

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The effect of cooking on the losses of ascorbic acid in vegetables and of activity of ascorbic acid oxidase was determined.
1. The loss of ascorbic acid in vegetables was greater in samples added in water of low temperature and cooked than those added in boiling water and then cooked. This tendency was observed significantly in ‘shungiku’.
2. The presence of ascorbic acid oxidase was observed in ‘shungiku’ brussel sprouts, and immature greenpeas. It appears that the enzyme which is contained in ‘shungiku’ and catalyses the oxidation of ascorbic acid is a mixture of catecholase and ascorbic acid oxidase. The loss of ascorbic acid while cooking was greater in vegetables havin the higher a activity of ascorbic acid oxidase.
3. The inactivated enzyme solution of ‘shungiku’ showed further some activity to destroy ascorbic acid. It seems that this is due to quinones formed from phenolic compounds by the action of catecholase during enzyme preparation.
4. When ‘shungiku’ was cooked in salted water the retention of ascorbic acid was observed to be greater than that obtained without salt, and this protecting effect of NaCl against ascorbic acid was only observed with NaCl solution of 1M or more over concentration.

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The changes in the amounts of mineral elements in the walls of the stomach and large intertine of the rachitic animals by this desease were not brought to be clear as those in the bone and liver. However, the amounts of sodium and potassium in the stomach wall of the rachitic animals were, without exception, significantly larger than those of the controlled animals, and the similar tendency was observed concerning those in the wall of large intestine.
Also, the calcium content in the walls of the two organs showed always the same tendency as Na and K.

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The amounts of calcium and phosphorus in the muscle of the rachitic animals were smaller, compared with those of the controlled animals, and the amounts of sodium, iron, and copper of the mal-nutritive rachitic animals and of potassium of the normal nutritive rachitic animals were larger than those of the controlled animals on the contrary to the cases of calcium and phosphorus.
The amounts of calcium, phosphorus and sodium in the spleen showed always larger amounts in the rachitic rats and rabbits than in the controlled animals, and high copper content was observed in this organ of rachitic rats and high potassium and iron in rabbits.

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The calcium amount in the heart and cerebrum of the rachitic animals was smaller than that of the controlled animals, and as to the phosphorus content, the same trend as calcium was observed expect the mal-nutritive rachitic animals.
The amounts of iron and copper in the lung and cerebrum of the rachitic animals were slightly larger, compared with those of the controlled animals.
However, the amounts of sodium and potassium in the heart of the mal-nutritive rachitic animals were considerably larger than those of the controlled animals.
But, the potassium content of the rabbits was only smaller than that of the controlled animals.

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