Eiyo To Shokuryo
Online ISSN : 1883-8863
ISSN-L : 0021-5376
Volume 21, Issue 2
Displaying 1-10 of 10 articles from this issue
  • Mitsuko Okada
    1968 Volume 21 Issue 2 Pages 71-73
    Published: 1968
    Released on J-STAGE: November 29, 2010
    JOURNAL FREE ACCESS
    Effect of ammonium sulfate on respiration was examined with the isolated rat liver mitochondrial system using pyruvate as substrate.
    A minute amount of ammonium sulfate caused a severe inhibition of respiration, and a decreased utilization of the substrate was observed at the same time. On the other hand, glutamate and aspartate productions after the ammonium sulfate addition were too small to explain the respiratory inhibition.
    The formation of α-ketoglutarate was inhibited by ammonium sulfate and the degree of the inhibition was closely related to a decrease in intramitochondrial pyridine nucleotide level. On the other hand, glutamate was largely produced by glutamate dehydrogenase reaction when α-ketoglutarate was added externally to the mitochondrial system or when flow rate of the TCA cycle was increased. In summary, ammonium sulfate inhibits respiration, while it is utilized as a nutrient for the glutamate synthesis under some different conditions.
    Download PDF (548K)
  • Mitsuko Okada
    1968 Volume 21 Issue 2 Pages 74-76
    Published: 1968
    Released on J-STAGE: November 29, 2010
    JOURNAL FREE ACCESS
    When mice were fed on a high protein diet (70% casein), there were increases in the levels of ornithine, urea and arginase activity. Inorganic ammonia, however, kept almost constant level regardless of protein content of the diet. Ornithine-keto acid transaminase (OKT) reacted with α-ketoglutarate formed by the TCA cycle to produce glutamate and glutamic semialdehyde by the addition of ornithine to isolated mitochondria. In this system glutamic semialdehyde was further oxidized to glutamate. The OKT-TCA cycle reaction was inhibited by valine which is a competitive inhibitor of OKT to ornithine, and by ammonia which inhibits the supply of α-ketoglutarate. Hepatic ornithine content was elevated by the injection of ornithine, valine or both.
    Download PDF (491K)
  • Kozo Ishiguro, Masayoshi Nakakuma, Katumi Uruno, Yoshio Noie
    1968 Volume 21 Issue 2 Pages 77-80
    Published: 1968
    Released on J-STAGE: November 29, 2010
    JOURNAL FREE ACCESS
    Blood specific gravity, serum protein and serum tryptophan content were low in inhabitants of mountainous areas than in those of farming-and fishing-areas, but serum protein fractions did not show any regional differences among inhabitants of these three areas.
    It was interesting that serum tryptophan concentration presented a positive correlation with that of serum gamma globulin and a negative correlation with that of serum albumin significantly. The data obtained in the present study were in acord with the results of rat experiment reported by Koyanagi and Ishiguro.
    Download PDF (696K)
  • Mitsuaki Maki, Yukio Sato
    1968 Volume 21 Issue 2 Pages 81-84
    Published: 1968
    Released on J-STAGE: November 29, 2010
    JOURNAL FREE ACCESS
    From the hot water extract of Osmunda Cinnamomea, the mucilage was precipitated with alcohol and prepared purely by cupric sulfate. As a result of chemical analysis, it was made clear that the mucilage consisted of the polysaccharides of pectin and hemicellulose combined with metal ions of calcium, magnesium, aluminium, potassium and sodium, had xylose, arabinose, glucose, galactose and galacturonic acid as the component sugars, and the content of moisture was 8.91%, ash 0.12%, total sugars (as glucose) 90.84% and methoxyl group 2.04%. Summarizing the facts above mentioned, the authors proposed some structural form of this mucilage.
    Download PDF (674K)
  • Kaneko Tanaka, Michiko Kurebayashi, Hirofumi Tanaka
    1968 Volume 21 Issue 2 Pages 85-88
    Published: 1968
    Released on J-STAGE: November 29, 2010
    JOURNAL FREE ACCESS
    The boron supply in the culture medium largely affects the ascorbic acid content of vegetable plants. There are two phases of the condition determining the amount of the acid in these biosynthesis and decomposition. The reaction between borate and polyhydroxy compounds, having been long used in carbohydrate chemistry and analytical chemistry, was supposed to be applied for the inhibition of the oxidation of vitamin C and its related compounds.
    Some experiments ware conducted to prove the above mentioned reaction, and the effectiveness of borate for the prevention of dehydroascorbic acid oxidation was proved.
    Dehydroascordic acid and 2, 3 diketo-L-gulonic acid, at pH7.4, were almost completely protected by borate against oxidation. This protection apparently depends upon a combination between the borate and the acids.
    Borate could not inhibit the oxidation reaction of L-ascorbic acid.
    Download PDF (722K)
  • Naoomi Ozaki, Keiko Yamada
    1968 Volume 21 Issue 2 Pages 89-93
    Published: 1968
    Released on J-STAGE: November 29, 2010
    JOURNAL FREE ACCESS
    A method for a determination of peroxide in lipids contained in food was tried and it was found that this method was practical. Outline of the authors' method is as follows.
    Lipids are extracted at room temperature with carbon tetrachloride (40ml), from food materials (10g), crushed with sodium sulfate anhydrous. Iodometry is applied to an adequate quantity (usually 10ml) of filtrate of carbon tetrachloride solution without removal of solvent, for avoid the destruction of peroxide by heating during the distillation. Same quantity of this filtrate is taken and the solvent is distilled out and the amount of lipids in this filtrate is measured. From values gained by these two measurements, peroxide value per unit weight of lipids is calculated.
    Download PDF (1103K)
  • 1968 Volume 21 Issue 2 Pages 94-110
    Published: 1968
    Released on J-STAGE: November 29, 2010
    JOURNAL FREE ACCESS
    Download PDF (4183K)
  • 1968 Volume 21 Issue 2 Pages 110-122
    Published: 1968
    Released on J-STAGE: November 29, 2010
    JOURNAL FREE ACCESS
    Download PDF (3178K)
  • 1968 Volume 21 Issue 2 Pages 122-135
    Published: 1968
    Released on J-STAGE: November 29, 2010
    JOURNAL FREE ACCESS
    Download PDF (3313K)
  • 1968 Volume 21 Issue 2 Pages 135-148
    Published: 1968
    Released on J-STAGE: November 29, 2010
    JOURNAL FREE ACCESS
    Download PDF (3416K)
feedback
Top