The fatty acid composition of the liver and adipose tissue from fasting rats were examined by means of gas chromatography. It has been proved that each fatty acid which constitutes phopholipids or neutral fats of the liver and adipose tissue changes its content individually through the course of starvation. The fact suggests that the fatty acids of some kinds of the lipid seem likely to serve in a di fferent manner respectively as an energy source of the body.

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Most of soybean paste “Miso”, one of daily protein sources by farm families, are home made andh ave diverse qualities depending on combination ratio of materials, the ageing condition etc.
Qualities of soybean pastes collected from 66 households in Saitama and Gumma Prefecture were examined from the viewpoint of identifying the formation of amines in their ageing process. Thepre sence of tyramine in some of those soybean pastes was recognized qualitatively by the paperchromato-graphy, followed by its isolation.

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In order to elucidate the contamination of animal products due to the nuclear tests and to know the distribution of Cs-137 in soft tissues of bulls, K and Cs-137 were measured in organs and muscles of 18 month old bulls under normal feeding conditions.
These organs and muscles were rumen, intestine, liver, kidney, pancreas, lung, heart etc., and 6 kinds of muscles of best quality for food.
Ca and Sr-90 contents in some of the organs were also assayed.
As the result, Sr-90 was detected only in the rumen, kidney and large intestine. On the contrary, Cs-137 was detected in amounts from 2 to 15 CU in all of the organs and muscles, although there was no definite pattern of distribution of Cs-137 in the tissues.

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Potato, wheat flour dough, onion, beet greens and whale meat were fried at 180°C for 3 minutes in cooking oils with different “foam extension” values.
The amounts of oil absorbed in these materials were higher with oils of large foam extension values, and in these cases vitamin C in the materials was much more decomposed than the case of oils with smaller foam extension values.
Decrease of minerals in whale meats was also remarkable in frying with oils of la rger foam extension.

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To investigate the influence of food constituents on the rancidification of oil, non-fat casein, gluten, albumin and soluble starch were mixed with distilled water (pH 6.0) individually or in combination, and the n admixed with oil, and the variation of peroxide value and acid value was measured with varying standing periods.
1. If the casein was mixed with oil in the ratio of more than 1:1, the peroxide value of oil raised with the standing period (day), but in the case of less than 1:5, the peroxide value remained in low.
2. When casein, starch and water were mixed with each other or altogether in equal quantities and co-existed with oil, the acid value increased markedly in the case of casei n and water, followed by the three component mixtures and also respective mixtures of casein, albumin and gluten with water. The amount of increase was highest in the case of casein, and it was followed by albumin and gluten.

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Die erste Ursache über die niedrige Verdaulichkeit des Eiweisses von Chlorella lassen sich vielleicht auf seine zähe Zellmembran zurückfuhren.
Also wird das getrocknete Chlorella mit den einigen Enzymemitteln, in denen Zellulase, Proteinase usw. enthalten sind, vor der kiinstliche Verdaungen des Eiweisses von Chlorella mit Pepsine behandelt.
Ab er zeigt keine Wirkung auf die Verdaulichkeit des Eiweisses mit einigen Behandlungen von den Enzymemitteln.

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The authors had reported previously that the amount of Sr-90 excreted by the rats fed on the high calcium diet had been higher than that of the low calcium diet group.
In this report, the relation between the Na-phytate intake and the excretion of Sr* and Ca metabolism was investigated, because of interfering action of phytic acid to calcium absorption.
When the amount of Sr* excreted in feces and urine and uptake on femur were observed, there was no significant difference between two groups fed on Na-phytate diet and control diet.
From the results of this experiment, it seems that the existence of an adequate amount of calcium in diet will diminish the interference of phytic acid on calcium metabolism.

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The studies were made to establish the reliable and simplified methods of hydrolyses of several food proteins for microbial assay of the amino acids composing these proteins. Assay using the titrations of the acid produced by the test organisms gave maximum values of the amino acids when the proteins were hydrolysed in sealed-tubes at 120°C for 1 hour for glycine, 2 hours for cystine, 3 hours for tyrosine, 4 hours for lysine and serine, 6 hours for methionine, leucine, isoleucine, aspartic acid, glutamic acid, histidine and proline, 8 hours for alanine and valine, 10 hours for arginine all with 4N hydrochloric acid, while 8 hours for threonine and phenylalanine both with 3N hydrochloric acid. Tryptophan was well assayed, with minimum loss, from a hydrolysate prepared by 15-hour sealed-tube hydrolysis at 120°C with 4N hydroxide solution, and the value obtained was compared with that determined by colorimetric method. Effects of the presence of varied quantities of starch upon the assay values of several amino acids were also examined following these hydrolysis procedures.
Twenty four food proteins were analyzed by these hydrolysis methods and their amino acid compositions were determined, comparing the lysine contents with those determined as available lysine by Carpenter's method.

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The ratio (K) of f_A to f_B is defined as Nutritional Balance Coefficient, where both f_A and f_B are the F. N. C. of certain foodstuffs or dishes.
1) The ratio K expresses numerically the nutritional balance between the two nutritional elements.
2) The nutritional balance and pattern about two elements of several groups of menu or food are characterized in the f_A-f_B-diagram.
3) The ratio of f_A to E_A is the coefficient corresponding to the cost of the foodstuff, and all the foods which have equal ratio are of the same cost.
4) Radiantly the straight lines can be drawn through zero point on the f_A-f_B-diagram, which indicate the points of the same cost. The figure thus obtained can be useful when the choice of menu is necessary within the limited amount of expense.

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1) The percentage ratio of amino acid contained in the food to the ideal amount per N 1g was calculated, and a list indicating the mutual balance of amino acid and the proteinscores was prepared.
2) The percentage ratio of amino acids contained in 100g foodstuff to the fundamental daily nutritional allowances was listed into the f_AAx-table.
This table off_AAx about amino acids is equivalent to the F. N. C. table previously reported.
3)K_AAx, i. e. the ratio of f_AAx. to f_prot, can indicate the irregularity in composition of amino acids in each food protein.
4) By applying the f_AAx-table, the protein scores of foodstuffs or cooked meals isgiven by the following equation: whereK_kN is a constant determined by Nitrogen constantN_xexclusively.
5) The error in protein score calculation following the equation was studied.
6) The value of f_AAx/f_prot, in the case of S_AAx=100is 100/K_kN, and its table corresponding to N_x was exemplified. In this case the protein score of food is always satisfied, so that K_AAxtable is useful.
An example of its application was given.

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Various kinds of buckwheat starch separated and refined by the method cited in the previous report were examined with an X-ray diffractometer and a Brabender's amylograph.
It has been recognized that the diffraction pattern derived from the starches of summer-harvested buckwheat usually belongs to the A-spectrum, while that from the starches of autumn?harvested buckwheat often belongs to the C_A-spectrum, the first fringe of B-spectrum being found though weak.
This result can be assumed to be due to the difference of the outer temperature in which the plants grew, because the starch of one of the summer kinds, cultured in autumn, was found on examination to have the first fringe very weak.
Besides, the amylograms of the starches, at 8% concentration, of the summer-harvested buckwheat compared with those of autumn-harvested, it has been found that the latter always shows a pasting point 3°C lower and a maximum viscosity higher than the former.

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An experiment on the weanling rats were proceeded to estimate the nutritional value of the utilization of skim-milk in the farm village diets of the Tohoku District (especially, eastern side) of Japan.
The farm village diets were divided into two groups by the protein content. Low and high protein diets contained 9.05% and 11.71% protein in average, and used as the experimental diet I and IV respectively. Experimental diets II and V were added skim-milk to the diets I and IV respectively to make the protein content of about 18%. Experimental diets HI and VI were added V. B₂ to the diets I and IV respectively to make its content as of the diets II and V.
Thirty-six weanling rats of Wister strain were divided into six groups I to VI.
Each groups consisted of six rats, three males and three females, and was fed with the corresponding experimental diet I to VI.
During and after 40 days feeding, the animals were examined on the following items as the indication of the nutritional values of these diets: increasing rate of body weight, efficiency ratio of diet, weight of liver, spleen and kidney, bone length and weight, calcification of bone, the liver fat content, the level of blood hemoglobin, serum alkaline phosphatase activity. The following results were concluded from the experiment.
The diets II and V had a higher nutritional value to growing rats than the other diets and the differences were statistically significant, especially in the point of view of body weight, visceral weight, bone length and content of minerals in bone. On the mean of the liver fat content, there was no significant difference among each group of different diet, except between diets I and V (I was higher than V). The low protein, low calcium diet seemed to have a contributing factor in liver fat deposition. The level of serum alkaline phosphatase of the rats of diets II and V were lower than that of the other groups. The differences were statistically significant. The level of blood hemoglobin seemed to reflect the amont of V. B₂ in diets.

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Rats were fed on several fat diets, and fats and fatty acids in serum were analysed.
When rats were fed on high-fat diets, large amounts of diglycerides, monoglycerides and fatty acids were absorbed. The absorption was largest when olive oil and linseed oil were fed and was least by depot fat of rats.
From above results it was suggested that the greatest incerase in absorption would be observed by simple saturated?triglycerides feeding and little change by mixed 2-unsaturated-1-saturated trigly-cerides feeding.

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The patient (29 years, housewife) suffered for a long time from typical vitamin B₂ group defficiencies, but from Feb. 1961 pantothenic acid defficient symptoms also appeared. Thereafter the pantothenic acid defficient symptoms appeared off and on for about a year and 9 months. She was then treated with various kinds and doses of pantothenic acid derivatives, such as, D-L (or D-)-alcium pantothenate or D-L (or D-)-pantothenol and the effect of each derivatives was observed.
1) 100mg of D-calcium pantothenate or D-pantothenol cured the symptoms readily. In the case of D-L-derivative 200mg had the same effectiveness.
2) When 100mg of D-calcium pantothenate or Dpantothenol was given in the early stage or reappearance of pantothenic acid defficient symptomes, the effectiveness was remarkable.
3) Pantothenic acid defficient symptomes and nicotinic acid defficient symptoms readily recovered when given orally lange amounts (100mg) of the defficient vitamins. On the other hand vitamin B₂ defficient symptoms required about one to several weeks' treatment and vitamin B1 defficient symptoms required several months treatment.

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