The amount of phosphorus in rice, though large in comparison with that of calcium, is not enough for the normal growth of rats and calcification of their bone. Moreover, the greater part of phosphorus in rice is phytin form of low digestibility. The authors have investigated the effect of supplement of protein and vitamin D to the rice diet on the availability of phosphorus in rice by rats.
The experiments provide an evidence that the addition of egg white to the rice diet greatly enhanced the phosphorus utilization as that of vitamin D. The phytase of small intestine has been activated by the supplement of egg white as well as vitamin D to the diet. The effect of egg white and vitamin D is cumulative. In contrast to the effect of egg white the addition of phosphate decreased the phytase activity.

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Urinary nitrogen level of rats was determined before, during, and after the first stress (avoidance conditioning) and the second stress (extinction) period for 60 days. The level increased rapidly with stress and reached maximum during the first stress which coincided with an anxiety behavior. And the level was kept during the second stress. It restored and decreased rapidly after the stress. The excretion per intake ratio increased rapidly reaching maximum during the first stress period in all animals. And gradually decreased during and after the second stress period. The increased excretion ratio indicates an increase in protein catabolism. Increased selection of protein induced by the emotional stress is assumed to be closely related to the increased protein metabolism.

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Urinary glucose level of rats was determined before, during, and after the first stress (avoidance conditioning) and the second stress (extinction) period for 65 days. The urinary glucose excretion did not increase during the first stress period. The increase appeared gradually during the second stress period and reached maximum soon after the stress was over. It did not restore completely to the normal level after the stress. It increased in all animals coincidentally with an anxiety behavior. The increase was 120% in average during the second stress, and was 276% at maximum after the stress. An increase in the urinary glucose was brought by the emotional stress, and is related closely to the decreased selection of glucide. Present experiment shows a glucosuria induced by the psychological stimuli, which was persistently resulted long after the termination of stimulation and the disappear·ance of anxiety.

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The toxicities of histamine and phenylethylamine in the rotten matsutake mushrooms were already reported in the last paper Part 7. Now kittens were used to make it sure how histamine and phenylethylamine would cause animals to vomit.
1) Kittens vomited immediately after histamine was given through their mouths, the least necessary quantity being 15mg/kg.
2) Phenylethylamine had nothing to do with the vomit. But it caused a delatation of kitten's eyes, the degree of which varied on the quantity of phenylethylamine.
These symptons were acute and did not last long.

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Micro-organisms separated from matsutake mushrooms were examined how they act on the putrefaction of matsutake mushrooms.
They turned out to be mostly soil micro-organisms. Nematoda were also found to play an important role in the disintegration especially of the fibrin.

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To clarify factors indispensable to the geening reaction of “Gobo”, authors tried an experiment by a “Tempura” model system.
Alkalinity of solution in “Koromo”, chlorogenic acid or its related compounds contained in “Tane”, and nitrogeneous compounds in “Tane” and “Koromo”, were indispensable factors to the greening reaction. The reaction proceeded even at above 100°C.

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When heated at 50°C-138°C, the rates of the greening reactions of Menzel solutions (pH 9.1) containing chlorogenic acid with its mole ratios 1: 1, 1: 2, 2: 1 to phenylalanine or leucine depend on the kind of amino acid, the mole ratios and the reaction temperature.
The changed colors of solutions heated were estimated by Xenon automatic fluorescent colorimeter, and specialized by dominant wavelength in the CIE system and by three attributes of color in the Munsell system.

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The eighteen amino acids were determined by microbiological analysis in buckwheat proteins, namely, albumin, globulin, prolamin and glutelin.
The protein fractions were found to differ from each other in their amino acid compositions. Especially prolamin contained more glutamic acid and proline and less glycine, aspartic acid, lysine, alanine, arginine and threonine than those in other protein fractions. In comparison with amino acid pattern of protein fractions in cereals belonging to Gramineae, albumin and globulin gave no significant difference, however, prolamin was similar to that in wheat and naked barley (subfam. Pooideae) and glutelin was similar to that in rice (subfam. Pharoideae) or in Japanese barnyard millet and foxtail millet (subfam. Panicoideae).

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According to the new concept of Nutritional Coefficientification, the method of calculating protein score was concretely indicated. The method was compared with the method by Izumi and the characteristics were pointed out.

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The concept of E.N.C. previously reported was revised on the coeffcient indicating the % value of daily nutritional allowance purchasable per 1 yen.
In applying this newly revised concept, the cost calculating slide ruler was devised. The daily E.N.C. value of school lunch in Kyoto city was calculated and compared with the value of home dishes.

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Many kinds of enriched (especially vitamin B₁ and B₂ fortified) foods, which have been approved by Ministry of welfare as “Tokushu Eiyo Shokuhin” (“Special Nutrition Foods”) are prevailing on the market.
Under these circumstances the survey was made for 298 items of B₁ enriched foods and 164 items of those B₂ enriched in Osaka area to find whether certein amounts of B₁ or B₂ mentioned to be added could be detectable or not.
The result showed that 23%, 43%, and 34% of total foods surveyed contained less 59%, 60-119%, and over 120% of B₁ respectively to the amounts of B₁ to be enriched.
The B₂ content in the food items surveyed showed similar variation as those of B1 enriched foods.

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The variation of the free amino acid contents in strawberry and Japanese persimmon (fuyu) during their growing period were investigated by the aid of ion exchange chromatography and paper partition chromatography.
The results obtained are listed in Table 2 and 3.

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Calcium, phosphorus, and iron content in Tsuruna (a vegetable) were determined. Calcium and phosphorus content were high in May and June, when calcium was above 90mg% and phosphorus was approximately 60mg%. Iron was approximately 3mg% regardless of sampling time.
The solubility of calcium, phosphorus and iron contained in Tsuruna was investigated by soaking and boiling in water. Also, the cooking losses of those minerals were investigated by boiling and soaking Tsuruna in water to get rid of the harshness. Moreover, the Tsuruna prepared as above was soaked In 0.5% HCl (HCl concentratian in gastric juice) at 37°C (bodily temperature) for two hours (staying time of food taken in the stomach) and the amounts of calcium, phosphorus and iron dissolved were determined to estimate the utilization of those minerals by the body.
The greater part of the calcium of Tsuruna was insoluble, and the cooking loss and the amount of calcium dissolved in HCl were small. The greater part of the phosphorus and iron of Tsuruna was soluble. Their cooking losses were so large that the amounts of those minerals dissolved in HCl were small.
Tsuruna was also compared with spinach and Kyôna in regard to the matter described above.

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Smith claims in 1942 that the creatinuria of women is exaggerated during premenstrual period, but he did not take the effect of diets into consideration. The authors reexamined the relation between the excretion of creatine and the periods of menstrual cycle with three women, who continued to take the same diets, throughout the experimental period.
The daily variation of the excretion of creatine has been so considerable that a definite relation between the creatinuria and the period during the menstrual cycle could not be found.
Contrary to expectation, the urinary estrogen also had not a constant relation with the menstrual cycle.

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A survey was performed for assessing the nutritional states of the children of the agricultural district who are living uncomfortably from the economical and cultural viewpoint. Body height and weight, nitrogen, creatinine and riboflavin in the urine, vitamin A and riboflavin in the blood, and the basal metabolism were determined.
Body height and weight of the children of the agricultural district were considerably inferior to that of the children of the urban district, but the Kaup's index for the former children was normal.
Nitrogen, creatinine and riboflavin seemed to be excreted in a small amount. However, thereby we can not draw a definite conclusion that they are deficient in protein and riboflavin.
A lesser amount of urinary creatinine and the inferior growth of body height and weight may be due to the pubertal spurt of the agricultural district get in later time as compared with that of the urban children. Further research needs to be done in this area. Basal metabolic rate was almost in normal range.
Contrary to speculation, we could not confirm that the children of the agricultural district were undernourished, so far as we had surveyed.

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For the coloring of water-base foods especially of juices and beverages with β-carotene, the oily emulsion of β-carotene is most preferable.
For this purpose, solubilities of β-carotene in various solvents were measured, obtaining the following results.
1. β-Carotene once dissolved in orange oil by heating remained about 3% in solution after standing at room temperature for as long as one month.
2. When the solution of β-carotene in orange oil was heated, isomerization and degradation occurred. In general, the higher the heating temperature and the longer the heating period, the more the cis compound was formed. When 1% suspension of all-trans β-carotene in orange oil was heated at 100° for 30 minutes, 60-65% remained as all-trans form, although 35-38% was isomerized to Neo-B.

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The coloring of soft drinks with emulsified β-carotene was studied with the following results.
1. The stability of emulsified β-carotene was practically excellent.
2. The presence of oxygen was the most important factor to affect the stability of β-carotene in the emulsion, and the light accelerated the destruction of β-carotene in the presence of oxygen.
3. Copper ion greatly affects the stability.
4. The orange soft drinks colored with the emulsion did not show any adverse effect.
5. Little color change was observed when orange soft drinks fortified with ascorbic acid was colored by the emulsion and stored at least for a year.

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The cholesterol level in the rat fed with supplemented lysine, tryptophan and methionine to 5% casein diet was examined, to know the predominent factor in cholesterol lowering effect of 30% casein diet. The result showed that these amino acids have no effect on the cholesterol level. And the authors observed an increase in Kiliani-reactive substances in the feces of rat fed with 30% casein diet which decreased the serum and organ cholesterol levels. It is assumed that the decrease in the cholesterol levels in the serum, adrenal, liver and lung were due to an increase in the fecal Kiliani-reactive substances.

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The effect of amino acids supplement of 5% casein diet on the serum cholesterol level in the rat was investigated. The cholesterol level was scarcely decreased in the rat fed with the diet supplemented with only essential amino acids. However, supplements of arginine, aspartic acid, cystine, glycine and glutamic acid in addition to the essential amino acids resulted in a remarkable decrease in the serum cholesterol level.

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Home-made soybean pastes used by farm families show occasionally the presence of tyramine but seldom in commercial “Miso”.
From the viewpoint of utilization of soybean as protein sources of farm families, an improvement of the quality of soybean pastes is an important problem in extension services.
In order to disclose the actual condition of the tyramine content distribution of home made soybean pastes 147 samples were collected from 147 farm households in Saitama, Tochigi and Gumma prefectures.
According to the assay results by paper-chromatographical and photometrical procedures, the range of tyramine content in these soybean pastes was from 0 to 53mg%. And among these soybean pastes the group that contains more than 30mg% tyramine accounted for 10% of the total.
However, the correlation between tyramine content and other individual factors such as duration of aging, sodium chloride content and ratio of materials could not be found.

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