When a small amount of lipid peroxide (hydroperoxide of ethyl linoleate) was fed (POV 100 or 250, 3.8% in diet) to rats, the effects of administration of some antioxidants (BHT, riboflavin tetrabutyrate, α-tocopheryl acetate and α-tocopheryl nicotinate) were examined. The oxidative hemolysis of red blood cell by dialuric acid test, TBA values of liver and blood plasma, and liver glutathione peroxidase activities were measured.
Obtained results were as follows.
1) Administration of lipid peroxide to rats increased level of oxidative hemolysis of red blood cell showing increment of vitamin E deficiency except vitamin E-supplemented rats. Supplementation of antioxidants except vitamin E did not show any effect on this increment of hemolysis.
2) Administration of lipid peroxide increased levels of TBA values in liver and plasma, but vitamin E administration inhibited to some degree the increase of TBA values, but did not decrease to the level of non-administered group of lipid peroxide supplemented with vitamin E.
3) Glutathione peroxidase activities in liver decreased by the administration of lipid peroxide and restored by the administration of vitamin E.
4) Glutathione peroxidase activities in liver was increased in vitamin E-deficient rats of non-administered group of lipid peroxide comparing with vitamin E-supplemented groups of the same condition. (Received April 14, 1980)
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