It was well known that thiamine was readily adsorbed on acid clay and that the adsorbed thiamine was not eluted from the adsorbent. In previous papers, the authors showed the adsorbed thiamine could readily be eluted by some protein or amino acids solution.
The present experiment was designed to clarify the availability of thiamine adsorbed on acid clay in rats.There was no difference between pure and adsorbed form in growth test. So was it in urinary excretion of both forms.It was concluded that no significant difference was found in the availability of these.

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The nutritional utility of protein of rice and bread was compared by the experiment on five male subjects 23-25 years old.Total experimental period was devided into three, the first period being 5 days as preparation and the second and the third being 10 days each, during which the subjects were fed rice or bread as a staple food each period alternatively. The side dishes were, however, prepared samely throughout the three experimental period containing 19.3 g animal protein per day.
Results obtained were as follows:
(1) On the digestibility of the protein, the bread period was superior to the rice period.
(2) On the nitrogen equilibrium, no difference wes obtained.
(3) On the ratio of accumulated nitrogen to intake or to absorbed nitrogen, the rice period was superior to the bread period.
(4) No differences were found between the two periods on the status of blood and physiological functions influenced by working, but on the specific gravity, haemoglobin content of blood and on some physiological functions, better results were obtained from a large number of the subjects during the rice period.

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The total choline content of 74 kinds of vegetables and mushrooms commonly used in Japan were determined.The purity of the choline reineckate obtained from plant materials was tested by piper chromatography.after hydrolysis with nitric acid The results were as follows:
1) Mushrooms, pulse crop, and herbage crops were generally rich in choline, especially, cauliflower (71.1mg%) and brussel sprout (83.6mg%) contained larger amounts of choline than other vegetables.
2) Arrow head, taro, yam, bambooshoot, and mushrooms, which are known as Asthmogene Nahrung, contain a large amount of choline.
3) The navel of broad bean contained 308mg%, therefore it was found that the choline content was the largest of all the plants examined.
4) The pureness of the choline reineckate obtained by this determination method, though there were a few exception, was further ascertained by paper chromatographic test.

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Riboflavin content of processed albuminous foods that is cheese, dried bonito, pidan, dried mullet roe, and paste of sea urchin eggs, were determined chemically. Free riboflavin and its esters were separated by paper partition chromatography, and their amounts were compared. The author found that the content of riboflavin in those foods was very low except in Chiai (dark muscle) of dried bonito, and that of esteribied riboflavin was small or none. The esters of riboflavin were supposed to shift to free riboflavin in the cource of processing.

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The nutritional effect of the addition of L-lysine and DL-threonine to polished rice was examined in rats fed on polished rice as the single protein source. Examinations were made on the increase of body weight (growth), nitrogen balance, activity of xanthine oxidase in the liver, total protein in the serum, and serum protein fraction. The growth of rats fed on polished rice added with both lysine and threonine was good but liver xanthine oxidase activity was extremely low like the group fed on diet without addition of the amino acids. No significant difference was found in total protein in the serum and serum protein fraction, as well as in nitrogen balance. These observations indicate that the addition of these amino acids alone to polished rice does not give a complete protein nutrition. It was also considered that evaluation of the nutritional value of proteins cannot be made sufficiently by one method but requires examinations by various methods.

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In order to have precise knowledge on the nutritive value of yeast protein, the ratswere fed the diets containing 42% and 90% of yeast grown on sulfite waste liquor of wood, and urinary all antoin-nitrogen and uric acid-nitrogen were estimated. These values were 50-70γ/mg intake N and 1-3γ/mg intake N, resp. and were about 5 times as much asthose from rats on dry whole egg feeding.
It is thought that the biological value of about 6% of total N of the yeast is zero, since about 6% of total N of the yeast is excreted in the form of all antoin-nitrogen in urine, so the biol. values of the yeast protein are calculated 75 at 5% level of protein intake and 68 at 10% level of protein intake from the data in the Part II of this report. These valuesare similar to those of animal proteins.

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The value of curd tension has a tendency to fall along with the decrease of proteinor casein content of the milk, although there are some cases in which the relation did notcome to existance.
This is greatly influenced by the state of combination of calcium andphosphorus to casein in the milk. A hard curd is formed when the ratio of combined Ca/P becomes low.Even the milk deficient in protein can form hard curd in the case oflow Ca/P ratio.The relation of curd tension value and natural acidity of milk was followed but withno remarkable significance.

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The iodine content of 53 main food-materials in Japan was determined.
(1) Seaweeds were generally rich in iodine. The highest value of 167 mg% was found indried Laminaria.
(2) The iodine content of fish and beef liver was relatively high (0.2mg%-O.9mg%), a small amount of iodine was detected in seeds, nuts, pulses, and polished rice (0.04-0.09mg%).

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It is said that Japan is the only non-goiterous conutry. This study is to estimate theiodine intake of Japanese. The daily iodine content in Japanese diets was about 200γ-20mg. The daily urinary excretion of iodine was about 200γ-7mg. Wide individual variationswere due to differences in seaweeds intake. From these results the iodine intake of Japanese is generally estimated to be about 500-1000γ or more daily. This value seemedto be very high than that of any other countries.

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From the standpoint of effect on keeping quality of milk, the comparison was madebetween low pressure polyethylene can and metal can as milk container.
Several trials of storage tests at room temperature (in summer) and low temperature (3°C) were carried out. Analyses were made on acidity, total bacterial count, vitamin C determination, organoleptic observation, and alcohol test. Samples were collected atintervals of 4 hours for 16 hours in room temperature and 24 hours for 120 hours in lowtemperature test, respectively.
Data obtained showed that there was no marked difference in effect on keeping qualityof milk between polyethylene can and metal can except for some superiority of theformer in bacterial count and flavor.

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During storage of raw starch, the deterioration of this occurrs and results in the depressions of yield and quality, but any adequate method for this prevention is not yetfound.
The authors reported already about the purification of potato starch by surface activeagents, and in this paper, the suppressing effect for the deterioration of raw potato starchby surface active agents is observed.
Raw starch treated with a dilute dodecyl benzene sulfonate solution posseses good stabilityfor deterioration and is kept from the lowering of pH value. By this means, a moreprolonged storage of raw potato starch may be possible Although the suppressing effect for deterioration depends upon the purity of the raw starch, a treatment with 0.05-0.15% of dodecyl benzene sulfonate is generally favorable.

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