The consumption of soysauce and its loss per person per day were investigated. The amount of loss of soysauce was calculated from the amount of salt examined in the foods or/and liquid remainded in used dishes and pans.The samples were collected from thirty meals in ten days of a certain dormitory.
It was observed that the average consumption of soysauce was 34.3 ml and the average ratio of its loss to the amount of the consumption was 9.6% with ranges from 3.9 to 17.9%.

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In the previous report, it was suggested that the non-boiled Koji-juice contained an inhibiting substance which could prolong the lag period of growth of Nukamiso Lactic acid bacteria. In this report its properties were investigated.
I. This substance was found to be extracted with butyl acetate from the non-boiled Koji-juice at pH 2.5 and transfered into sodium bicarbonate solution.
2. The inhibitory substance in the extract was lost its activity by heating for one hour in the following condition: at pH 3.0, 100°C, pH 6.8, I00°C, or pH 7.0, 60°C, respectively.
3. Koji mold could produced this inhibitory substance in surface culture of Modified, Mayer medium, but not in shaking culture.

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An investigation was made on the diets among the 30 Katori settled families extracted arbitrarily from the 56 ones. Since each one of the settlers (who were the object of this survey) showed much more nutrients intake than the average one for farmer in the other parts of this country, the economic conditions of these families seem to be good as for settlers of the newly cultivated land. But as some of them (23.3%) took no animal proteins, it was assumed that there was an extreme difference between them. This could be also recognize from their consumption of seasonings, fishes, and its products. The mode of their living differs very much between them by the various, complicated conditions of location, soil, weather and farming plan; consequently, for the promotion of their diets, more systematic investigations must be undertaken in this settled village.

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It was observed that the nutritive value of average Japanese diet can be significantly improved by supplementing 0.2% l-lysine and 0.2% dl-threonine. A group of six rats was fed with dried mixed diet of 2.1 % nitrogen content and another group with casein diet. There was no difference in growth between the two groups and also the effects of the amino acids supplementation could not be observed.
However, the results showed that the nutritive value of the same dried mixed diet but diluted with corn starch to a 0.8% nitrogen level was significantly lower than that of casein diet with a same nitrogen level. At this level of nitrogen, the effect of the supplementation with lysine and threonine to this mixed diet can be observed in growth of weanling rats, protein efficiency and the xanthine oxidase activity of liver. The supplementation with lysine alone to the mixed diet promoted the growth of rats in the first 3-4 weeks but did not in a later period.

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When vitamin A, absorbed previously on defatted soybean flours, was mixed with another materials at the beginning of manufacturing process, the loss of vitamin A in this “Miso” was 10% approximately after fermentation, and the decrease of vitamin A content during the storage for 2 months at 27°C. was very slight.
Though the dispersion of vitamin A in “Miso” was not uniform at the early period of fermentation, the uniformity became high in the products owing to the tank-change process repeated several times during the fermentation and crushing.
On the other hand, the direct addition of vitamin oil onto nonfortified “Miso” was carried out easily by the use of a suitable mixer, and the high uniformity of vitamin A dispersion was obtained in spite of the simple addition. The loss of vitamin A in this “Miso” was 2-3%, after 2 months storage.

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1. Five radioactive calcium salts, mixed with normal rats ration, were administered to rats research the availability of these calcium. After 3 days of feeding with those rations, radioassay values were obtained with one side of the femurs of those experimental rats.
2. Carbonate and Lactate were found to be the most effective salt for the deposition of Ca on femurs, and the efficiency of phosphate and phytate was aproximately half times of above two salts, while little effect with oxalate.
3. These findings suggested that calcium carbonate and lactate were the most suitable materials for the preparation of enriched foods on calcium.

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The effect of ascorbic acid on the decoloration of various dye solutions was studied. Results are as follows;
(1) The rate of decoloration of dye solutions increased as the reaction period was prolonged.
(2) The rate of decoloration was increased, with the increwie of ascorbic acid concentration, but not in proportion to that concentration.
(3) The decomposition of dye solutions was affected by light in the experimental conditions.
(4) There was no obvious change on the colors, when the ethylendiaminetetraacetate known as inhibitor for the oxidation of ascorbic acid, was added to the mixture, and similar results were obtained, when dehydroascorbic acid was used instead of ascorbic acid in the reaction mixtures. From these results, it was assumed that the decoloration was due to the oxidation of ascorbic acid to dehydroascorbic acid.

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Further studies were made, with the following results, on the lag period of the growth of lactic acid bacteria which was prolonged by a substance contained in the non-boild Koji-juice.
1. The lag phase was reduced by the addition of phytin to an extent of 10γ per ml to the non-boiled Koji-juice medium, and the bacterial growth was much promoted in all phases, when more phytin was added. However, effects both of shortening the lag period and of promoting the growth were not shown by inositol. Inorganic phosphate was found, by the addition to an extent of 1236γ per ml to the medium, to shorten the lag period, but not promote the growth. This series of experiments, therefore, revealed the fact that phytin specially showed the promoting effect on the growth of lactic acid bacteria, and this result was a noteworthy one.
2. Yeast-RNA also had the lag-shortening effect, but its decomposed compound, mononucleotide, had not.

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The l-ascorbic acid content of green bean seedlings increased with the germination, when the plant was grown on the media with SH inhibitors, such as p-chloromercuribenzoate (PCMB), NaASO₂, and CH₂ICOOH, but this was reverse with SH compounds such as reduced glutathione (GSH), and cysteine. On the contrary the formation of l-ascorbic acid from D-glucurono-lactone, which is said to be the precursor of that vitamin during the germination was inhibited by the presence of SH inhibitors such as PCMB; but SH compounds such as GSH or cysteine increased this biosynthesis.

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1) In the lumen of rat small intestine, D-glucurono lactone was converted to l-ascorbic acid, and this was promoted by the presence of SH compounds, such as glutathionine (GSH) or cysteine, but inhibited by P-chloromercuri benzoate (PCMB), NaAsO₂, or CH, ICOOH.
2) In the case of ascorbic acid formation from d-glucose, Robinson-ester, or Harden Young-ester neither of SH compounds nor their inhibitors were observed to have this effect.
3) Yet the biosynthesis of l-ascorbic acid from Cori-ester was conspicuously accelerated by oxidized glutathione (GSSG) or PCMB, and inhibited by GSH and cysteine.
4) These results suggest us that the phosphorylation of D-glucose to Cori-ester is the first and direct pathway of the biosynthesis of l-ascorbic acid from D-glucose in intestine.

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The mineral contents of the mother's milk, which was taken by the rachitic infants, differed greatly among the individuals, so that the definite tendency could not obtain. However, generally speaking, the amounts of the total calcium, dialytic calcium, phosphorus, sodium, and potassium were comparatively greater in the rachitic mother's milk, and those of the copper, and iron were a little smaller than those of the normal mother's milk.

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Owing to the individual differences, the amounts of the minerals were scattered greatly in the feces of the rachitic infants who had taken the non-artificial feeding. However, the contents of the calcium, phosphorus, iron, and copper were generally greater and that of the potassium showed a reverse tendency, comparing with these contents in the feces of the normal infants.

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The effect of thiamine on β-amylase of sweet-potato was studied, and the results obtained are as follows:
1. About 20% inhibition of the activity of β-amylase was caused by the addition of thiamine at the concentration of 10^-3M, but no inhibition took place at the concentration of 1/3×10^-3M or at less concentration.
2. The activity of β-amylase was not inhibited by pyrimidine derivative and thiazol, or by the mixture of them, but it was inhibited by thiochrome at the concentration equal to that of 10^-3M thiamine.
These results seem to suggest that the combination of pyrimidin and thiazol is essential for the inhibition of β-amylase activity by thiamine.

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Studies were made on the effects that Vitamin B₂, B₆, B₁₂, Nicotinic acid (N. A.), Vitamin B₁+B₂, B₁+B₆, B₁+B₁₂, and B₁+N. A. were to give upon β-Amylase of sweet-potatoes.
The results were as follows:
1) The activity of β-Amylase was inhibited only little, if any, by the single application of B₂, B₆, B₁₆ and N. A. but the inhibition was much greater than by the single application of B₁, when B₁ was mixed severally with B₂, with B₆, with B₁₂, and with N. A.
2) The mixture of B₁ and B₁₂ slightly reduced the inhibition of the activity of β-Amylase caused by Vitamin B₁.

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The nitric acid hydrolysis method established by C. J. Ackerman is a simplified method for the estimation of choline in biological material which eliminates the troublesome extraction with methanol and permits the analysis of both wet and dry samples, but in his case the recovery of choline added to the test sample ranged from 97.8 to 106.4 per cent, and the deviation of resuts were somewhat greater.Therefore several conditions for the imprivement of this method were investigated.Results are as follows:
1) Washing with n-propanol results in the formation of Na₂CO₃ and NaNO₃ that tends to loss of choline.
2) When the test sample is hydrolyzed at about 80°C, a better result can obtain.
3) The presence of glycine, proline, hydroxyproline and certain amino acids do not interfere on this analysis.
The results of the recovery test carried out according to a new procedure fell on the same ranges with the original method, but the deviation was smaller than that of original one.

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