Tocopherol (Toc) content of egg yolks was determined by the TLC-Colorimetric method of Tsugo et al. and some factors affecting their content were investigated. The results were followings.
1. The average Toc content of 40 egg yolks from seven sources were : α-Toc 19.3±7.6, γ-Toc 10.0±4.0μg/g. There is a great variation of Toc content among eggs. δ-Toc content was 0.3 μpg on an average, but in many cases below 0.1. The ratio of α-Toc/γ-Toc was 2 on an average and deviated from 1 to 3.
2. The consecutive eggs from a hen had almost the same content of Toc, but there was a large variation among eggs from different hens of a same flock.
3. Single oral dose of each 30 mg of α-, γ- and δ-Toc to a hen gave a maximum value of her egg Toc content after 5 days and greatly transferred into the egg yolks after 4-6 days. Amount of α-, γ- and δ-Toc transferred into egg yolks were each 18.0%, 4.8% and 1.0% of Toc dosed.
4. Giving 10mg/day of α-Toc acetate to a hen daily, Toc content of egg yolks was definite after the 12th day and the transferring efficiency (egg yolk Toc/Toc given) was 18.2 on the average but deviated among individual hens.
5. Shell eggs which were wrapped in aluminum foil and stored in a sealed container at 15°C, showed no change of Toc content during storage of 23 days.

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To clarify the properties of wheat gluten flour blended to wheat flour, Food Rheometer was applied for the measurement of the strain and stress for a dough sheet, when it is ruptured by stretching under constant rate of load.
The relation among stress h, strain a, and extensibility product S (≈ h·a) of the dough sheet and loaf volume of the bread were observed.
High correlation was observed between extensibility product and loaf volume of bread, on baking the bread with (1) wheat flour, (2) wheat flour enriched with spray-dried wheat gluten, (3) wheat flour blended with other proteins than wheat protein.
It was shown that this method can be applied for the study on properties of wheat gluten products in dough formation.

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Niacin intake of adult women in Osaka Prefecture was surveied in 1972-3. Ages of the subjects were from 18 years to 40 years. Eighty-six of them were picked out from the sample of the national nutrition survey in 1972, and fifteen of them were students of Osaka City University. Mean niacin intake was 17.0±5.48 mg/day/person, 8.21 ± 2.14 mg/1, 000 kcal, and mean niacin equivalents (niacin plus one sixtieth of tryptophan) was 33.1 ±9.49 mg/day/person, and 15.9 ± 3.75 mg/1, 000 kcal. Niacin equivalents were met the Japanese nutrition allowance by most of these subjects. Niacin intake and niacin equivalents were correlated with protein or calorie intake on this survey.

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One of the authors has reported that a certain amount of cadmium accumulated in cuttlefish liver has no injurious effect on an animal whereas the same amount of cadmium chloride is injurious. To make clear this reason a study was carried out on the measurement of the complex formation between cadmium and glutamic acid by means of the paper electrophoresis, using the radioisotope of ¹⁰⁹Cd as a tracer. The results obtained were compared with the stability constants of cadmium glutamate complexes reported by other authors, and further discussions were made. For example, in 0.1 M of glutamic acid (H₂L) solution at pH 6.0, the composition ratios of cadmium species were as follows : Cd²⁺, 52.42%; CdL, 46.75%; and CdL₂^2-, 0.83%.

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Tyrosinase inhibitors in cow liver were extracted with 0.25 M sucrose containing 10-3M ethylendiamine tetraacetic acid. Subsequently, they were partially purified by centrifugation (105, 000 X g, 60min), heat treatment (100°C, 10 min) and gel-filtration with a Sephadex G-25 column. The propertiesof the partially purified tyrosinase inhibitors were studied and the following results were obtained : 1. Molecular weight of a tyrosinase inhibitor in cow liver was estimated to be less than 5, 000. 2. This tyrosinase inhibitory activity was stable against the heat treatment and unaffected by treatment with p-chloromercuri benzoic acid.
3. The inhibitor showed the maximum absorption at about 260 mμ and the minimum absorption at 225 mμ.

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Male rats of the Wistar strain, weighing about 42g were fed 70% casein diet with or without vitamin B₆. Growth rate of rats fed vitamin B₆ dificient diet was markedly retarded. Liver enlargement and fat deposition, which resulted from increases in triglyceride and cholesterol, were observed in the vitamin B₆ deficient rats. Liver weights and fatty acid compositions in the normal rat livers were changed during 19 hour starvation, whereas those of the deficient rat livers were not changed during the same starvation period. Impaired oxidation of fatty acids in the deficient rat will be suggested. Fatty acid compositions of carcass lipid were determined and an increase in arachidonic acid content in the deficient rat was remarkable.

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The antioxidative activities of distilled watersoluble and ethanol-soluble fractions of 10 kinds of spices and the synergistic effects of the extracts with a-tocopherol were examined in lard system at 98°C.
The ground spices showed antioxidative effects. Among them, rosemary and sage showed pronounced antioxidative effects. Distilled water soluble fractions from all spices except clove did not show antioxidative effects. Ethanol-soluble fractions from all of the spices used showed antioxidative activities as high as those of ground spices when employed at equivalent concentrations. The greater part of potent compounds of ground spices appeared to be extracted with ethanol. Distilled water-soluble fractions from all of the spices used showed synergistic effects with a-tocopherol. Ethanolsoluble fractions from mace and clove wereeffective.

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