Separation of the green pigments prepared by the reaction of chlorogenic acid with nitrogen-containing compounds in alkaline sodium hydrogen carbonate solution and in Menzel buffer solution, was investigated by means of Sephadex G-25, G-15 column chromatography, using distilled water or Menzel buffer solution as eluent.
Using distilled water as eluent, blue-green, purple, and yellow pigments were separated from the green solution obtained by the reaction of alanine, phenylalanine, or glutamic acid with chlorogenic acid in alkaline sodium hydrogen carbonate solution. And, from the reaction mixture of phenylalanine with chlorogenic acid using Menzel buffer solution as eluent, green and yellow pigments were separated, and, using distilled water as eluent, blue-green and purple pigments were obtained.
Similar results was obtained in the reaction products of phenylalanine and chlorogenic acid in Menzel buffer.

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Dietary habits and health complaints were studied for 553 employed workers of both sexes in a variety of industries by the questionnaire sheets; one was for dietary habits, and the other was for health complaints (the standardized questionnaire designated Todai Health Index).
The results obtained were as follow,
1) The balanced dietary intake was associated with the balanced body weight toward stature.
2) Among those who had unbalanced dietary intake were persons living alone.
3) The better mental-physical health status in the response to Todai Health Index appears to be associated with the appropriate food-eating behavior.

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Human milk fat (H), cow's milk fat (M), fractionated beef tallow (B), fractionated palm oil (P), coconut oil (C), lard (L), interesterified lard (LE) and slightly hydrogenated lard (LH) were digested in vitro with steapsin and separated into the oily and micellar fractions.
The total amounts of monoglycerides (MG), free fatty acids (FFA) and salt forms of fatty acids (FA salts) solubilized into micellar solutions increased in the order of C>H>M H>L>P LE>B. The differences were only significant between C and the others, and between B and C, H or M. The variance in LH was relatively large.
The solubilization ratio of each fatty acid into micellar solution increased in the order of C_12:0>C_14:0>C_16:0>C_18:0 and C_18:2 C_18:1>C_18:0.
The solubilization ratio of C_14:0, C_16:0 or C_18:0 was greater in fats in which the fatty acid distributed preferentially to 2-position.

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To study the influence of nondigestive fiber on the intestinal transit and alimentary canal, male mice were given the diet containing 0 to 30% cellulose for 9 weeks. The size of small intestine and caecum were not affected by cellulose intake but those of stomach and large intestine (colon+rectum) were affected. Namely the length and weight of stomach increased significantly at 30% cellulose content, and the length and weight of large intestine also showed the tendency to increase following to increasing cellulose content. The shortening of transit time, and the decrease of fecal water content were observed with the increase in the dietary cellulose content.

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How did the variation of dietary life in recent years result in changes of the nutrient intakes of the preschool children living in a mountain-, a farm-, a fishing-area, and a suburb quarter of Niigata prefecture, during a five year period? The present study was carried out on each group of children (10-25 in number) by living environments. The dietary surveys for obtaining information on actual food consumption were done using a weighing method for three days running for each season twice during a five-year-interval from 1968 to 1977.
1) The change of the food constitution pattern of children in the mountain- and the fishing-area was greater as compared to that in other areas. The nutrient intake patterns in four districts changed during past five years. On the whole their nutritional status is changing for the better.
2) The increase in intakes of meat, fats and oils, and vegetables (excluding green and yellow colored ones) along with the decrease in intake of confectioneries, yet poor intakes of gren leafy vegetables, milk and cereals were observed commonly among all the children in the four districts.
3) The intakes of animal protein and fat of the children in the suburb quarter, and the ones of total and animal protein of the children in the fishing-area showed a tendency of over-intake. The children in the mountain- and the farm-area were still insufficient in intakes of animal protein, fat, calcium, vitamin A, thiamine and riboflavin.

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Sucrase-isomaltase complex (S-I) was purified from rat intestinal mucosa and its properties were observed in comparison with those of other species.
1. Rat intestinal S-I having maltase activity was purified from papain solubilized intestinal mucosa by the following ammonium sulfate fractionation, Sephadex G-200 column chromatography and DEAE Sephadex A-25 column chromatography.
2. The molecular weight of rat intestinal S-I was estimated as 215, 000 by polyacrylamide disc gel electrophoresis.
3. Km values of sucrase, isomaltase and maltase activities of the complex were 18.0, 4.0 and 3.7mm, respectively. These enzyme activities were competitively inhibited by Tris. Furthermore, these enzyme activities were inhibited by heavy metal ions either completely (Ag⁺, Hg⁺⁺) or partially (Cu⁺⁺, Zn⁺⁺). Optimal pH was 6.0.
The properties of rat intestinal S-I were similar to those of other species.

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In order to know the relationship between AOM values and the autoxidation temperature, AOM tests were carried out with lard, beef tallow, corn oil and palm oil without the addition of antioxidant under three different temperatures besides the changes in POV and CoV were investigated. The results obtained were as follows:
1) In the AOM tests at the same reaction temperature, POV began to rise in the order of lard, corn oil, beef tallow and palm oil. While the rate of this rise was comparatively fast in lard and beef tallow, and POV rose rapidly, on the other hand, the rise of POV was slightly in corn oil and palm oil slower. However, the time required to reach POV 100 in beef tallow was faster than corn oil.
2) When the reaction temperature was below 100°C, AOM values can be expressed as the exponential function of the reaction temperature in all fats tested but in the case of corn oil slightly different result was obtained comparing with other fats, and this suggested that AOM values among beef tallow and corn oil could be reversed by certain reaction temperature.
3) CoV during AOM test tended to rise later than POV and its changes were much smaller than that of POV. A good correlation was noticed between POV and CoV during AOM tests for all the fats and oils.

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