Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
Volume 54, Issue 4
Displaying 1-41 of 41 articles from this issue
  • Yoshiko UCHIDA, Manami MIZUTANI, Takuya KUBO, Tetsuya NAKADE, Kanjuro ...
    1992 Volume 54 Issue 4 Pages 611-614
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    Eight beagles were experimentally inoculated intraotally with Malassezia pachydermatis to induce acute otitis externa. Three or 4 days after the inoculation, the animals showed the symptoms of otitis externa. All ear canals were erythematous and the dogs were shaking their heads. A large number of M. pachydermatis was noticed in exudate taken from every ear canal. Clinical signs of otitis externa were reduced after treatment with 0.1 ml (per canal) of 1% pimaricin suspension twice a day for 3 days. The amount of exudate decreased gradually and 12 of the 16 ear swabs examined, thereafter, were found to be negative for M. pachydermatis within 10 days. No side effects were observed in all the treated cases. These results suggested that M. pachydermatis could induce the canine otitis externa, and that pimaricin is effective agent for M. pachydermatis infection in ear canals.
    Download PDF (548K)
  • Ryohei NISHIMURA, Minoru SAKAGUCHI, Manabu MOCHIZUKI, Nobuo SASAKI, Hi ...
    1992 Volume 54 Issue 4 Pages 615-620
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    The effects of intramuscular injections of xylazine (2mg/kg)-ketamine (15mg/kg) [X-K15], and xylazine (2mg/kg)-ketamine (5mg/kg)-butorphanol (0.22mg/kg) [X-K5-B] were compared in atropinized (0.05mg/kg) miniature pigs (pigs). Both combinations induced the anesthesia for more than 1 hr, however X-K5-B induced the more potent and well balanced anesthesia as compared with X-K15, although the amount of ketamine was reduced to one third. The duration of loss of pedal reflex, an indicator of surgical anesthesia, in X-K5-B (62±13min) was significantly (P<0.05) longer than X-K15 (28±19min). In addition, X-K5-B was accompanied by loss of laryngeal reflex in all pigs. Recovery from anesthesia in X-K5-B was much smoother than in X-K15, and the administration of yohimbine (0.05mg/kg) could rapidly and smoothly reverse the anesthesia induced by X-K5-B, although it was accompanied by a transient fall in blood pressure and tachycardia. The combination of xylazine, ketamine and butorphanol appears to be a relatively safe and widely available anesthesia for the period of one hour in pigs.
    Download PDF (943K)
  • Akihiro OISHI, Hiroshi SAKAMOTO, Ryosuke SHIMIZU, Fumihito OHASHI, Aki ...
    1992 Volume 54 Issue 4 Pages 621-628
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    Measurement of plasma erythropoietin level in normal dogs by in vivo bioassay has been considered to be impossible so far. In the present study, we successfully determined it by using concentrated plasma 60 times which allowed the lower limit to 2.7 mU/ml. This normal plasma erythropoietin level was the first to be determined as an in vivo bioactivity and was 9.14±7.81 mU/ml in 75 normal adult dogs. This value was sufficiently reliable in terms of accuracy of determination and considered to be meaningful as the low level in vivo bioactivity that hasn't been known to date. Furthermore, erythropoietin levels in normal plasma were within a certain lower range and showed neither difference in plasma erythropoietin level between males and females or among breeds nor correlation between erythropoietin and hemoglobin level.
    Download PDF (987K)
  • Kazuro MIYAHARA, Shinichi TOSE, Haruhisa SAKURAI, Ikuo IGARASHI, Atsus ...
    1992 Volume 54 Issue 4 Pages 629-635
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    In order to identify mechanisms responsible for the anti-tumor effects of Toxoplasma lysate antigen (TLA), we used an in vitro 51Cr release assay to study the functional properties of plastic-adherent cells during induction of splenic cytotoxic activity by TLA. Cytotoxic activity of non-adherent cells was measured in all experiments after a 6 days incubation. Induction of cytotoxic non-adherent cells by TLA required the presence of plastic-adherent spleen cells. In contrast, rhIL-2 alone was able to induce transformation of cytotoxic non-adherent cells from non-adherent spleen cells. Contact between adherent and non-adherent spleen cells was necessary for successful induction of cytotoxic non-adherent cells by TLA. Treatment of spleen cells with anti-macrophage serum prevented induction of cytotoxic activity by TLA. Biologically active IL-2 was not detected in culture supernatants of spleen cells exposed to TLA. These findings suggest that contact between TLA-sensitized non-adherent cells and macrophages is necessary for induction of cytotoxic cells in the presence of TLA. This contact, however, is not necessary for generation of IL-2-induced killer cells.
    Download PDF (888K)
  • Masayoshi KUWAHARA, Hisashi HIROSE, Kunio DOI, Shigeru SUGANO
    1992 Volume 54 Issue 4 Pages 637-642
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    This study was designed to assess the effect of hyperlipidemia on cardiovascular responses to adrenergic stimulation in a porcine model. Four-week-old piglets (n=10) were divided into two groups; one fed a control diet and the other was fed an atherogenic diet for 8 weeks. Cardiovascular responses were evaluated to both norepinephrine (NE; 0.5 and 2.5μg/kg) and isoproterenol (ISO; 0.1 and 0.5μg/kg) from simultaneous recordings of femoral arterial pressure, heart rate, left intraventricular pressure and left intraventricular dP/dt. It was found that no significant difference in the baseline values of cardiovascular function was observed between the control and hyperlipidemic groups. However, in the hyperlipidemic group as compared with the control group: 1) arterial blood pressure responses to NE were significantly increased (P<0.05), 2) cardiac contractile responses to NE and ISO were significantly potentiated (P<0.05), and 3) reflex bradycardia in response to increasing arterial blood pressure did not occur. These findings indicate that hyperlipidemia can potentiate the cardiovascular responses to adrenergic stimulation, whereas reflex cardiovascular regulation is somewhat altered by hyperlipidemia. Conceivably, these observations may have relevance to the possible role of the mechanism of the interaction of hyperlipidemia and hypertension in atherogenesis.
    Download PDF (853K)
  • Minoru SAKAGUCHI, Ryohei NISHIMURA, Nobuo SASAKI, Toshikazu ISHIGURO, ...
    1992 Volume 54 Issue 4 Pages 643-647
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    Sedative effects of medetomidine, a potent selective and specific α2-adrenoceptor agonist, were evaluated in pigs using 5 different doses (30, 50, 80, 100 and 150μg/kg of body weight) and compared with those of xylazine (2mg/kg). Atropine (25μg/kg) was mixed with both drugs to prevent severe bradycardia. All drugs were administered intramuscularly. Medetomidine at a dosage of 30μg/kg produced more potent sedation than xylazine. The depth of sedation induced by medetomidine was dose dependent within the range from 30 to 80μg/kg. At 100 or 150μg/kg, the depth of sedation was mostly the similar level to that at 80μg/kg but the duration was prolonged. The degree of muscle relaxation produced by medetomidine also seemed to be dose dependent from 30 to 80μg/kg and was stronger than that produced by xylazine. An increase in the duration of muscle relaxation was dose dependent up to 150μg/kg. No analgesic effect was produced by xylazine, however moderate analgesia was obtained by medetomidine. There were no marked changes in heart rate and respiratory rate during the observation period in pigs of any groups, however mild hypothermia after the administration of both drugs was observed. From these results, medetomidine has a significant and dose-dependent sedative effects which are much more potent than that of xylazine, and a combination of 80μg/kg of medetomidine and 25μg/kg of atropine is suitable for sedation with lateral recumbency and moderate muscle relaxation without notable side effects in pigs.
    Download PDF (715K)
  • Yasuyuki SANADA, Hiroshi NODA, Hajime NAGAHARA
    1992 Volume 54 Issue 4 Pages 649-652
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    Serological survey of Rhodococcus equi infection in horses in Hokkaido was performed using ELISA. Of 2, 879 horse sera, 318 (11.0%) gave antibody-positive (OD≥0.3) reactions. The antibody-positive rate of female was significantly higher (p<0.01) than that of male, and no statistical difference between Anglo-Arab and thoroughbred was detected in the antibody-positive rate. The maximum antibody-positive rate (27.1%) was shown at 14 years of age. The antibody-positive rates on the 160 farms were found to vary widely from 0 to 78.9%. A significant difference (p<0.01) in the antibody-positive rate was detected among horse farms. It was elucidated that 100 (62.5%) out of 160 horse farms had an antibody-positive horse. These results indicate that R. equi was widespread on horse farms, and the level of environmental contamination with R. equi differed among horse farms.
    Download PDF (560K)
  • Minoru TAKEUCHI, Hiroshi SHIBATA, Tetsuyuki NASU
    1992 Volume 54 Issue 4 Pages 653-658
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    Effect of soft x-ray irradiation on immunological functions in mice was investigated. Soft x-ray irradiation with 100R or more induced a significant reduction in the number of plaque-forming cells (PFC). The reduction in the number of PFC depended on the irradiation doses. Irradiation with 600R or more showed a significant reduction in the delayed reaction of footpad swelling. However, soft x-ray irradiation with doses ranging from 100R to 1000R did not exert significant influence on the K values of carbon clearance test. Irradiation with 100R or more of soft x-ray showed a remarkable reduction of response to concanavalinA (ConA) or lipopolisaccharide (LPS) in spleen cells, and the response to ConA was lower than that to LPS. These results suggest that in the soft x-ray-irradiated mice, antibody-producing ability, delayed type hypersensitivity reaction and mitogenic activity are sensitive to soft x-ray irradiation and furthermore, T cell is more sensitive than B cell, but phagocytic activity of reticulo-endothelial system (RES) is resistant to soft x-ray irradiation.
    Download PDF (720K)
  • Kazuyuki UCHIDA, Yoshiro TANI, Koji UETSUKA, Hiroyuki NAKAYAMA, Naoaki ...
    1992 Volume 54 Issue 4 Pages 659-667
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    Amyloid protein was isolated from the cerebral meninges of 4 aged dogs with cerebral amyloid angiopathy. By immunoblot analysis, antiserum against synthetic oligo-peptide consisting of 1-28 amino acid of amyloid beta protein recognized prominent wide band ranging from 14 to 18 kilodalton (kd). When amyloid samples were solubilized by formic acid, the antiserum recognized lower molecular weight band ranging from 3 to 4 kd. Immunohistochemical studies on cerebral amyloid angiopathy and senile plaques were performed in 17 aged dogs. Anti-amyloid beta protein serum labeled amyloid deposits in cerebral vessel walls and senile plaques. Compact deposits of beta protein were detected in primitive or classical plaques. After using formic acid pretreatment, diffuse deposits of beta protein in the neuropil representing diffuse plaques were detectable. Classical and primitive plaques reacted with antiserum against glial fibrillary acidic protein, while not with antisera against alpha 1-antichymotrypsin, IgG and IgM. Amyloid deposits in the intestines of aged dogs examined, did not react with anti-amyloid beta protein serum.
    Download PDF (1753K)
  • Shin-ichiro KATSUDA, Hiroshi HOSOMI, Masashi SHIOMI, Yoshio WATANABE
    1992 Volume 54 Issue 4 Pages 669-673
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    Effects of atherosclerosis on the mean value and daily variation of arterial pressure were studied in 12 Watanabe-heritable hyperlipidemic (WHHL) rabbits aged 12 to 35 months and 25 normal Japanese white rabbits aged 6 to 30 months. A pressure catheter was inserted through the left subclavian artery under pentobarbital anesthesia. A few days after the catheterization, the mean arterial pressure (MAP) of the rabbits, which were active and in a good state of appetite, was recorded by an analogue-to-digital converter every second for about 6 hrs and stored in a computer. The mean (M) and standard deviation (SD) in the WHHL rabbit, calculated from each successive MAP record, ranged widely from 85.8 to 131. 4 mmHg and 5.6 to 12.6 mmHg, respectively. There was no significant correlation between M and SD in the WHHL rabbit. M and variance (V) of MAP in the WHHL rabbit were significantly higher than those in the normal rabbit. M did not show any significant change with increasing ages, whereas SD increased significantly with aging in the WHHL rabbit. Concentrations of serum total cholesterol and triglyceride in the WHHL rabbit were 475 and 328 mg/dl, which were about nine and seven times as high as those in the normal rabbit, respectively. Macroscopic and histopathological examinations of the aorta revealed development and spread of sclerotic lesions with aging in the WHHL rabbit. We can conclude that development of atherosclerosis with aging in the WHHL rabbit causes malfunction of the baroreceptors, which contributes to hypertension and lability of arterial pressure.
    Download PDF (911K)
  • Hiroyuki SUNAGAWA, Katsuhiro INOUE
    1992 Volume 54 Issue 4 Pages 675-684
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    Toxin-converting phages CEβ and d-16φ and non-converting phages CEγ and d-1', isolated from toxigenic strains C-468 and D-CB16 of Clostridium botulinum types C and D, respectively, were characterized biologically and biophysically. DNAs isolated from these four phages were studied physicochemically. Phages CEβ, d-16φ, CEγ and d-1' were adsorbed to their susceptible cells at the constant rates of 1.1×10-8, 3.3×10-8, 1.4×10-8 and 1.4×10-8 ml/min, and grew after latent periods of 35, 45, 35 and 35 min at the burst sizes of 38, 85, 35 and 35, respectively. Converting phages were more susceptible than non-converting phages to physical and chemical treatments such as temperature, pH, time, UV-irradiation and organic solvents. GC% of phage DNAs were determined by melting temperature, buoyant density and HPLC analysis to be 26, 26, 29 and 29% for CEβ, d-16φ, CEγ and d-1' phage DNAs, respectively. The restriction digestion profiles of phage DNAs with seven endonucleases were compared by agarose gel electrophoresis, revealing that the two converting phage DNAs were similar in regard to five enzyme digestion profiles, but different in the other two enzymatic profiles. Non-converting phage DNAs produced the same restriction digestion profiles with all seven endonucleases. The molecular sizes determined from the size of restriction enzyme digestion fragments were about 110 kb for converting phage CEβ and d-16φ DNAs and 65 kb for non-converting phage CEγ and d-1' DNAs. Dot blot hybridization experiments revealed that DNA homology between the converting phages CEβ and d-16φ was 50-75%, while that between non-converting phages CEγ and d-1' was about 100%. Converting phage and non-converting phage DNAs did not hybridize at all.
    Download PDF (1308K)
  • Morimasa YAMANAKA, Kazuhisa HIRAMATSU, Tadashi HIRAHARA, Tatsuji OKABE ...
    1992 Volume 54 Issue 4 Pages 685-692
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    We investigated pathological changes at the injection site in guinea pigs and rats for 16 weeks following a single intramuscular injection of one of the following oil adjuvant emulsions; oil adjuvant ISA-70, Freund's incomplete adjuvant, Freund's complete adjuvant, and aluminum phosphate gel. In the animals injected with ISA-70 emulsion prepared by manual shaking, grossly, there was partial thickening of subcutaneous tissue, discoloration of inter-muscular connective tissue, and swelling of the inguinal lymph nodes at 2 and 4 weeks post injection (PI). Histopathologically, ISA-70 injected sites revealed acute inflammatory changes at 72 hrs PI, and peak reactions consisting of macrophage accumulation around oil cysts and fibrosis were observed at 4 weeks PI. These changes were less severe and of shorter duration than those in the other three adjuvants. Guinea pigs and rats injected with materials containing inactivated Newcastle disease virus (NDV) antigen similarly showed an infiltration of plasma cells and lymphocytes in addition to the changes described above. ISA-70 containing NDV antigen induced similar hemagglutination-inhibition titer to that induced by Freund's incomplete adjuvant.
    Download PDF (2427K)
  • Hiroaki HASEBE, Fernando A. OSORIO, Alex HOGG, Hardi LIAUW, Michael J. ...
    1992 Volume 54 Issue 4 Pages 693-698
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    In order to compare the effect of the route of immunization on the efficacy of a modified live Aujeszky's disease (AD) vaccine, which had deletions in both thymidine kinase (TK-) and glycoprotein gIII genes (gpIII-), 20 six-week-old pigs were vaccinated by either the intramuscular (IM) (n=10) or subcutaneous (SC) (n=10) route. All the animals, including five non-vaccinated control animals, were challenged with virulent AD virus 22 days after vaccination. Four of five non-vaccinated animals died within 12 days after challenge. Although none of vaccinated animals died, three of animals in the SC group exhibited clinical signs, and average daily gains in the SC group were depressed. The animals in the IM group were not found to shed challenge virus, but those in the SC group shed the virus up to 9 days. Virus neutralizaing antibody titers in the vaccinated animals were low or non-detectable by 21 days after vaccination. A glycoprotein gII (gpII) screening ELISA detected gpII antibody in all animals in the IM group. While, only 30% of animals in the SC group were positive by the same test. The results of this study indicate that TK-, gpIII modified live AD virus vaccine is effective against challenge with virulent AD virus; however, vaccination by the SC route reduced vaccine efficacy in comparison with IM route.
    Download PDF (778K)
  • Yoshimi BENNO, Akemi IZUMI KUROTANI, Masamichi YAMASHITA
    1992 Volume 54 Issue 4 Pages 699-702
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    The bacteria in the large intestines of eight Japanese tree frogs (Hlya japonica) were enumerated by using an anaerobic culture system. The microorganisms at approximately 3.1×109 bacteria per g (wet weight) of intestinal contents were present in the intestine of all the frogs tested. No difference of the total bacteria in the frog intestine was observed between two different incubation-temperatures (room temperature and 37°C). Eleven genera and 16 species were isolated from the frog intestine. In most frogs, Bacteroides (B.) caccae and B. vulgatus were detected as the predominant organisms. Escherichia coli was also present in greater numbers in the intestine. Other bacteria isolated at high dilutions were strict anaerobes, including Fusobacterium and Clostridium. Enterococcus faecalis was frequently isolated from the frog intestine. However, four genera of Bifidobacterium, Eubacterium, Peptostreptococcus, and Lactobacillus were not isolated from the frog intestine.
    Download PDF (643K)
  • Yoshimi BENNO, Hiroyuki NAKAO, Kiyohisa UCHIDA, Tomotari MITSUOKA
    1992 Volume 54 Issue 4 Pages 703-706
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    The gastrointestinal microflora of male beagle dogs in two different age groups; I) less than month 12 of age, and II) more than year 11 of age, was compared. No detectable difference occurred on the microflora of stomach, duodenum, jejunum, and ileum in both dogs. Large bowel (cecum, colon and rectum) microflora in both dogs yielded the different microbial populations. In all regions of large bowel, the levels of bacteroides, eubacteria, peptostreptococci, bifidobacteria, lactobacilli, and staphylococci in the elderly dogs were lower than those in the younger animals, whereas the numbers of Clostridium perfringens and streptococci in the elderly animals were higher than those in the youngers. The high incidence of lecithinase-negative clostridia was observed with advances in age, but not that of spiral shaped rods. The result of this study shows that the advances in age of beagle dogs yield some changes in the microbial population of large bowel in the animals.
    Download PDF (639K)
  • Toyotsugu NAKAI, Kazuyoshi KAWAHARA, Hirofumi DANBARA, Katsumi KUME
    1992 Volume 54 Issue 4 Pages 707-710
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    Two monoclonal antibodies (MAbs), lMAb-1 and lMAb-5, against Actinobacillus pleuropneumoniae serotype 1 were obtained. In enzyme-linked immunosorbent assay-inhibition tests with whole cell antigens obtained from serotype 1 to 12 strains of A. pleuropneumoniae, lMAb-1 reacted to only a serotype 1, strain 4074. The epitope recognized by lMALb-1 was a carbohydrate sensitive to periodate oxidation and resided on capsular polysaccharide (CP) of A. pleuropneumoniae serotype 1. On the other hand, lMAb-5 reacted with serotype 1, 9 and 11 strains at the same degree and its epitope was found to be located on O-polysaccharide of serotype 1, 9 or 11 lipopolysaccharide (LPS). These results showed that CP was one of the serotype-specific antigens of A. pleuropneurnoniae, and that O-polysaccharide of LPS obtained from serotype 1, 9 or 11 strain was the cross-reacting antigen among these strains.
    Download PDF (619K)
  • Jun SUZUKI, Hirokazu TSUBONE, Shigeru SUGANO
    1992 Volume 54 Issue 4 Pages 711-716
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    Bipolar or unipolar epicardial and intraseptum electrograms were recorded from the in situ rat hearts with spontaneous sinus rhythm or myocardial electrical stimulation of 400 bpm in order to investigate the sequence and duration of cardiac activation in the rat. The ventricular epicardial isochronous maps in the rat showed the activation sequence comparable to those in humans and dogs, although the activation time (6 to 13 msec) of epicardium was approximately one-third of those of such species. The delay of activation between septum and ventricular surface in the rat was 5 to 8 msec, which was also shorter as compared with those in humans and dogs. However, the conduction velocity of the ventricular epicardium in the rat was approximately 40 cm/sec, mostly equal to that of humans. These findings might be accounted for by the possible presence of the bundle branch-purkinje system, at least functionally, similar to humans. The local QT interval in the septum were relatively longer (69 to 123 msec), compared with that of the ventricular surface (50 to 98 msec), and such duratioris of ventricular epicardium and septum were considerably less than those of dogs and humans. These results are consistent with the configuration of QRS-T on the limb lead ECG.
    Download PDF (774K)
  • Takatsugu YAMADA, Motoo MATSUDA, Yoshinori ASHIDA, Ryo TSUCHIYA, Yasun ...
    1992 Volume 54 Issue 4 Pages 717-721
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    Secretory IgA was isolated from feline bile, using ammonium sulphate precipitation, gel-filtration chromatography and affinity chromatography. It formed a precipitating line between anti-cat whole serum or anti-cat bile serum by immunoelectrophoresis and double diffusion. It consisted of three subunits that molecular weight were 80 kd, 62 kd and 27 kd by SDS-PAGE analysis. The 80 kd protein was equivalent to secretory component of human and other animals. Immunoglobulin levels were observed on bile collected continuously by gallbladder cannulation in growing cats. Although immunoglobulins were not detected in bile of feline fetus, after birth its levels increased gradually. Biliary IgA levels reached the adult levels earlier than that of their serum.
    Download PDF (824K)
  • Toshi WATANABE
    1992 Volume 54 Issue 4 Pages 723-729
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    In the present study, attempts were made to evaluate the effects of the estrous cycle and the administration of estradiol (Ovx-E) or progesterone (Ovx-P) after ovariectomy on the threshold of antidromic (AD) and orthodromic (OD) responses of neurons in the medial preoptic area (POA) induced by electrical stimulation of the median eminence-arcuate nucleus (ARC) of the hypothalamus. Single units were evaluated with extracellular recording. The threshold of AD and OD response in POA neurons declined during estrus and proestrus of the estrous cycle and in the Ovx-E group, but increased in the Ovx-P group and during diestrus. The number of neurons with the inhibitory OD response increased during proestrus and in the Ovx-E group, but decreased during diestrus. The neurons with the excitatory OD response increased in the Ovx-P group and during diestrus, and decreased during proestrus. The threshold of the inhibitory and excitatory OD response decreased during proestrus, estrus and in the Ovx-E group, but increased in the Ovx-P group. It is apparent from these studies that estrogen appears to activate the neurons of the POA receiving inhibitory synaptic input via the axonal collateral branches of the ARC neurons, and that progesterone seems to activate the neurons of the POA receiving excitatory synaptic input.
    Download PDF (1011K)
  • Motoaki OUCHI, Mitsuo FUJIWARA, Yoshihiro HATANO, Masao YAMADA, Shiro ...
    1992 Volume 54 Issue 4 Pages 731-737
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    Seventeen monoclonal antibodies (MAbs) against swinepox virus (SPV) were produced and characterized. These MAbs were classified into eight groups (A through H) on the basis of the molecular weight of the polypeptides which they recognized and the staining patterns of antigens in SPV-infected cells by the indirect immunofluorescent (IF) technique. The MAbs belonging to groups A, B, C and G recognized late antigens in cytoplasmic inclusion bodies with molecular weights of 97 kD, 65 kD, 48 kD and 15 kD, respectively. The MAbs belonging to groups D and H respectively recognized 35 kD and 12 kD late antigens, which first appeared in cytoblasmic inclusion bodies and spread to the cytoplasms and surface membranes of the infected cells. The MAb of group F recognized an 18 kD late antigen with granular distribution in the cytoplasm. The MAbs of group E recognized a 32 kD early antigen. Although all the MAbs belonging to the six groups (A, D through H) were specific for SPV, some of those belonging to groups B and C showed cross-reactivity with members of the other genera of poxviridae. An MAb in group B, SP14, cross-reacted with orf and rabbit fibroma viruses. Two MAbs in group C, SP24 and SP32, cross-reacted with vaccinia, cowpox, ectromelia, and rabbit fibroma viruses. These findings indicate that at least two SPV antigens contain cross-reactive epitopes with different genera of poxviridae.
    Download PDF (989K)
  • Yoshihide SASAKI, Hitoshi KITAGAWA, Yuji HIRANO
    1992 Volume 54 Issue 4 Pages 739-744
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    The relationship between pulmonary hypertension and lesions was examined in 41 dogs infested naturally with heartworms, which consisted of 28 cases with pulmonary heartworm disease and 13 cases with caval syndrome. Pulmonary arterial pressure (PAP) was measured before and 1 or 7 days after heartworm removal with a flexible alligator forceps. In these dogs, lesions were examined after the last measurement of PAP. The mean PAP was 28.2+16.0 mmHg (10.9 to 81.4 mmHg in range) at post-removal phase. Pulmonary arterial intimal lesions, pulmonary thromboemboli, pneumonic lesions, tricuspid valvular lesions and mitral valvular lesions were macroscopically recognized in 95, 59, 39, 54 and 56% of cases, respectively. These lesions were classified by severity and the relationship with PAP was examined by the multiple correlation analysis. The multiple coefficient correlation was found the highest between PAP and thromboembolij followed by mitral valvular lesion, tricuspid valvular lesion, and pneumonic lesion. There was no significant correlation between PAP and intimal lesions. The coefficient of determination showed the highest value in thromboemboli when one variable was used, and increased only very slightly when a variable of thromboemboli was added to those of other lesions. The cases with high PAP had fresh thromboemboli in large pulmonary arteries. From these evidences, it was concluded that thromboemboli following natural death of heartworm was the most important factor causing an increase in PAP and developing clinical signs in canine heartworm disease.
    Download PDF (814K)
  • Hiroshi IKADAI, Junko NOGUCHI, Midori YOSHIDA, Tomonori IMAMICHI
    1992 Volume 54 Issue 4 Pages 745-749
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    A newly identified aspermia rat mutant was investigated on testicular histology and mode of inheritance of the defect. Average testis weight of mutants was about one-third of that of phenotypically normal males. Spermatogenesis was interrupted at meiosis. Pachytene spermatocytes significantly decreased in number. Secondary spermatocytes and few round spermatids were seen, but no elongated spermatids and sperms were observed. A large basophilic inclusion-like body existed in the cytoplasm of late pachytene spermatocytes. Genetic analysis revealed an autosomal recessive transmission of the defect. Aspermia (As) was designated for the locus.
    Download PDF (840K)
  • Hitoshi KITAGAWA, Atsuko KUBOTA, Kyoji YASUDA, Yuji HIRANO, Yoshihide ...
    1992 Volume 54 Issue 4 Pages 751-756
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    Cardiopulmonary function was examined in 18 dogs with serious chronic heartworm disease showing ascites, subcutaneous edema, prostration, weakness, jaundice and so on. After surgical heartworm removal from the pulmonary arteries, 10 dogs recovered (surviving group), and 8 dogs died or were euthanatized because of poor prognosis (nonsurviving group). The number of live heartworms residing in the pulmonary arteries of the surviving group tended to be larger than that in the nonsurviving group. At necropsy, severe pulmonary arterial lesions such as thromboembolism including dead heartworms, proliferative and villous lesions and intimal hyperplasia were noticed in all dogs examined, and tended to be severer in the nonsurviving group. Heartworm-coiling around the tricuspid valve chord was found in 1 dog of the surviving group and 4 dogs of the nonsurviving group. Before heartworm removal, there was no significant difference in the mean pulmonary arterial pressure (MPAP) between the surviving and nonsurviving group. Right atrial pressure (v-wave) was higher, and the cardiac index (CI) was lower in the nonsurviving group. Arterial oxygen tension was lower in the surviving group than in the heartworm-free group, and it was lower in the nonsurviving group than in the surviving group. Carbon dioxide tension was lower in the surviving group than in the heartworm-free group. Bicarbonate concentration (HCO3-) was lower both in the surviving and nonsurviving groups than in the heartworm-free group. One week after heartworm removal, MPAP decreased (P<0.05), and CI and HCO3- tended to increase in the surviving group.
    Download PDF (808K)
  • Mhan-Pyo YANG, Ryo GOITSUKA, Hajime TSUJIMOTO, Misao ONUMA, Atsuhiko H ...
    1992 Volume 54 Issue 4 Pages 757-761
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
    The association of tumor-associated antigen (TAA) on the proliferation of BLV-infected lymphoblastoid B-cell lines (BL2M3 and BL312) was investigated. Flow cytometric analysis of the expression of TAA with monoclonal antibody (mAb) c143 showed high expression of TAA on the surfaces of BL2M3 and BL312 cells. A large amount of TAA was found in the culture supernatant of BL2M3 and BL312 cells as well as in the lysates of BL2M3 and BL312 cells. Culture supernatant but not lysates of BL2M3 and BL312 cells promoted the growth of either BL2M3 cells or BL312 cells. Furthermore, this growth promoting activity in culture supernatants of BL2M3 and BL312 cells was inhibited in a dose-dependent manner when cultured with mAb c143. These results suggested that TAA may be involved in the growth factor-mediated cell growth of bovine B-lymphoblastoid cell lines expressing TAA on their cell surface.
    Download PDF (738K)
  • Takeshige OTOI, Susumu TACHIKAWA, Shoji KONDO, Tatsuyuki SUZUKI
    1992 Volume 54 Issue 4 Pages 763-765
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
  • Yukio SEIMIYA, Kan-ichi OHSHIMA, Hiroshi ITOH, Nobuyuki OGASAWARA, Mas ...
    1992 Volume 54 Issue 4 Pages 767-768
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
  • Hiroyuki NAKAYAMA, Kazuyuki UCHIDA, Sang-Koo LEE, Koji UETSUKA, Atsuhi ...
    1992 Volume 54 Issue 4 Pages 769-771
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
  • Harrison CHITAMBO, Akira ARAKAWA
    1992 Volume 54 Issue 4 Pages 773-775
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
  • Yutaka TAMURA, Mayumi KIJIMA, Khoko HAMAMOTO, Haruo YOSHIMURA
    1992 Volume 54 Issue 4 Pages 777-778
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
  • Takaaki YAMAMURA, Yoshio MINATO, Akihiro KOJIMA, Azusa OKANIWA
    1992 Volume 54 Issue 4 Pages 779-780
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
  • Hiroshi IKADAI, Motokazu TACHIBANA, Eiji KOBAYASHI, Tomonori IMANICHI
    1992 Volume 54 Issue 4 Pages 781-782
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
  • Yasuhiko YAMAGISHI, Osamu KATSUTA, Minoru TSUCHITANI
    1992 Volume 54 Issue 4 Pages 783-785
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
  • Kenji TSUKAMOTO, Makoto HASEBE, Shin-ichiro KAKITA, Hiroshi HIHARA, Yu ...
    1992 Volume 54 Issue 4 Pages 787-788
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
  • Shinogu HASEGAWA, Toshinori SAKA, Naoyuki TAKEMURA, Hidekazu KOYAMA, S ...
    1992 Volume 54 Issue 4 Pages 789-790
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
  • Piyanoot PRASITIRATANA, Tasanee CHOMPOOCHAN, Shigeyoshi URAI, Noriyuki ...
    1992 Volume 54 Issue 4 Pages 791-792
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
  • Michiko KONO, Yasuhiro FUJII, Sojin SHIKANO, Kenichiro ONO, Naoyoshi S ...
    1992 Volume 54 Issue 4 Pages 793-795
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
  • Hiromitsu ORIMA, Michio FUJITA, Sinobu AOKI, Makoto WASHIZU, Tetsushi ...
    1992 Volume 54 Issue 4 Pages 797-798
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
  • Yoshiyuki TAKAHASHI, Mohammad NIHAYAH, Mohamed R. JAINUDEEN, Mitsugu H ...
    1992 Volume 54 Issue 4 Pages 799-801
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
  • Ryu ITOH, Yukio KAGABU, Fumio ITOH
    1992 Volume 54 Issue 4 Pages 803-804
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
  • Mitsugu HISHINUMA, Etsuko HISHINUMA, Yoshiyuki TAKAHASHI, Hiroshi KANA ...
    1992 Volume 54 Issue 4 Pages 805-808
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
  • Kazuyuki UCHIDA, Hiroyuki NAKAYAMA, Nobuo SASAKI, Susumu TATEYAMA, Nao ...
    1992 Volume 54 Issue 4 Pages 809-811
    Published: August 15, 1992
    Released on J-STAGE: February 15, 2008
    JOURNAL FREE ACCESS
feedback
Top