The reversion-inducing cysteine-rich protein with Kazal motifs (
RECK) gene is one of the endogenous matrix metalloproteinase (MMP) inhibitors. It was reported that decreased
RECK expression closely correlated with tumor malignancy. We determined the cDNA sequence of the canine
RECK gene. The cDNA sequence and deduced amino acid of canine
RECK were 2,913 bases and 971 residues, respectively. The predicted amino acid sequence of the protein showed 95.5% and 91.9% homology with human and mouse
RECK, respectively.
RECK mRNA expression was analyzed in various canine tissues and tumor cell lines by quantitative RT-PCR. The highest
RECK expression was detected in lung and testis. In comparison with the tissues, a remarkably low expression level was detected in tumor cell lines. In addition, the
RECK gene was transfected in the canine transitional cell carcinoma, and its influence on cell proliferation, migration, and invasion was analyzed. The transfected
RECK gene suppressed only canine tumor invasion. These results showed that
RECK might play an important role in tumor malignancy in dogs as well as in other mammalians.
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