The presence of argyrophil cell was demonstrated in the adenomas of familial polyposis coli (FPC) with the Sevier Munger method. Ususlly, argyrophil cells were distributed sparsely near the base of the crypts, rather close to the normal mucosa. But, adenomas with severe or moderate dysplasia did not contain argyrophil cells. The frequency of argyrophil cells in the adenoma was remarkably higher in the sigmoid than in the ascending colon. Because this phenomenon was found both in the non-adenomatous mucosal crypts of FPC and of normal control cases, it is conceivable that the appearance of the argyrophil cells in the adenomas of FPC is affected by the structural arrangement of the original non-tumorous mucosa, as a background. Furthermore, a tendency, that many argyrophil cells were located within the adenomas, was observed in one of the seven families examined. It showed that the argyrophil cell which is thought to be one of the elements constituting the adenoma might actively participate in the growth of the adenoma.
Of late microbiological air samplers of various types have been developed in monitoring the critical areas in the hospitals and pharmaceutical plants. It has not been clarified, however, that a commercial air sampler is the most suitable for such a purpose. The present studies were conducted to investigate the bacterial collection efficiency of these air samplers.
The new experimental apparatus basically consists of a bacterial aerosol generator and an isokinetic sampling steel air duct. Serratia marcescens was used as the test bacteria, and then the bacterial collection efficiency of the three kinds of commercial air samplers (Andersen air sampler, Pin-hole air sampler and M/G air sampler) was examined.
It was found that in these experiments these three air samplers had a high bacterial collection efficiency. All except 0.3 to 2.0% of the small bacterial particles (1 to 5 µm) were trapped by these tested air samplers. Furthermore, in these three air samplers it was also confirmed that for collecting the hospital airborne bacteria the bacterial collection efficiency was more than 99.9%.
The authors’ findings showed that these three air samplers were disigned according to Ranz and Wong s theoretical and experimental results.
The study on the immunosuppressive activity of the cytoplasmic component (SF) from Streptococcus mitis on the immune response of mice to the sheep red blood cells (SRBC) was carried out. The bacterial cell suspension was disrupted in a Braun shaker. The disrupted cell suspension was diluted and centrifuged at 10,000xg for 30 minutes to remove the cell walls. The supernatant was centrifuged at 31,000×g for 45 minutes and the final supernatant was dialyzed against distilled water, frozen and lyophilized. These products were employed as the cytoplasmic component (SF). The SF in the extracts of Streptococcus mitis suppresses the antibody formation in mice against SRBC. It is effective to inject it just one day before the inoculation with SRBC.