Morphological and histochemical observations were made of the otolith membrane of the macula utriculi in normal guineapigs.The influences of fixatives upon the structure of the otolith membrane were observed with several kinds of fixatives.
The histochemical stainings were mainly applied to identify the acid mucopolysaccharides. Several of main findings were as follows:
1) The colloidal substance of the otolith me- mbrane shows P.A.S.positive reaction, metachro- matic reaction of toluidin blue and is stained with colloidal iron(after Rienhalt and Abul-Haj's me- thod).And these chemical character is resistent to hyaluronidase.The author belibues from these observations that the colloidal substance of the oto- lith membrane consists mainly of the hyaluronidase resistent sulfomucopolysaccharides.
2) The cilia of the sensory cells is situated in the colloidal substance, however the special mor- phological relation between the cilia and the colloidal substance is not distinctly observed.
3) The otoliths are covered with the organic substance which shows the chemical characters as colloidal substance.Electron microscope shros the otolith itself consists of a number of electron dense fine granules of about 100A and the organic matrix with low density.
4) As a fivative, absolute alcohol seemed to be most suitable for morphological observation of the otolith membrane.The metamorphosis of the otolith in various pH of the fixatives can be attri- buted to the result of the chemical transfiguration of the matrix in the otolith and the organic substance which covers the otolith.

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A case with the progressive gangrenous rhinitis was reported.He was a 34-year-old male who unfortunately expired following intestinal bleeding with peritonitis-like symptoms.Improvement in the general condition was the initial step by the administration of medicines and then surgey, radi- ation therapy and steroid hormone treatment were used and a somewhat clinically effective improve- ment in the symptoms was obtained but in using the radiation therapy and the steroid hormones we must take note of the nature of the "iatrogenic disease".We reported the 7 cases with the pro- gressive gangrenous rhinitis seen from 1955 to 1961
(5 cases had already died and 2 cases are yet alive) who consulted the OtorhinolaryngologicaI
Dept.of Osaka University Hospital.According to their clinical findings and examinations, this disease should be suspected when ulceration and granulation, in nasal cavity or accessory nasal cavity, remittent fever(resistant to antibiotics), continual accerelation. of sedimentation rate and no swelling of lymphno des, no specific findings in bacteriology and serology are found.And in such cases it was much better- to improve the general condition and then take- surgical and radiation means immediately.It was- concluded that Survival or follow-up result of this- disease shonld be studied further.

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A series of studies on the venous olfactory test were perfoumed and the results were as follows: (1) Initial and terminal time of olfactory senses surveyed by venous injection of Alinamin(Vitamin B1 preparate) (a) Normal:9.3sec., 170sec.Hyposmia:11.1sec, 60, 5sec.so called Anosmia:13.9sec, 48sec.(the former:initial time, the latter:terminal time) (b) The longer terminal time was seen in more Sensitive olfactory mucous membrance.
(2) According to previous olfactory test, dysos- mia was classified into two groups, i.e., hyposmia and anosmia
Each of which, according that test, is classified into two subgroups, thus dysosmia is classified into four groups, from I-degree to IV -degree.
(3) According to the region of disturbance of sense of smell, olfactory disturbance is divided into three types;
(a) morphorgic type
(b) neurogenic type
(c) combined type A critical clinical study of each type was also performed.
(4) Olfactograph based on olfactory dilution test and venous olfactory test.(initial time, termi- nal time) was proposed.
(5) With the use of the authoir olfactory test, the process of healing of dysosmia could be divided into three types.From these results, it was con- cluded that a careful study of the healing process was helpful in evaluating the prognosis and therapy.

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Serum mucoprotein(MP)by Weimer & Mos- hin's method of 61 patients suffering from tonsillitis chronica simplex, focalis and from tonsillitis chro- nica believed to have focal infection were determi- ned.The influence on MP 24 hours after the patients were given tonsillar ultra-short wave direct irradiation was also studied.
The results obtained were as follows:
1) Normal MP level was shown in the patients of tonsillitis chronica simplex, while high MP level was seen in those suffering from tonsillitis chronica focalis(especially rheumatic diseases).But in the group of nephritis and slight fever, MP level was lower than in the group of rheumatic diseases.
2) The increase of MP level after provocation was observed in 80% of the rheumatic diseases and 72.7% of the nephritis group, while only 55.6% was seen in those with slight fever.
3) It can be said recently that the determin- ation of MP is useful for specific diagnosis of rhe- umatic disease, because MP level increases before other clinical signs when it changes for the worse. So determination of MP could be said to be most suitable in showing any aggravation of rheu- matic disease after provocation.The finding of renal biopsy and renal function are related to MP level, so the aggravation of nephritis after provo- cation could be known by determination of MP.
4) In the disorders that were guessed to be the focal infection but were clearly not so, little increase of MP after provocation was observed.

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Authors observed swallowing in II cases, (by cinefluorography) who were hemiglossectomized in lingual cancer.
Swallowing disorders in these cases were caused by the insufficient of glossopalatinal closure due to lack of the half of the tongue and the injuries of neck muscle due to radical neck dissection.
But we could not find the remarkable differe- nces in the swallowing after hemiglossectomy bet- ween total hemiglossectomized cases and the cases where the root remained because the compensatory movement of the rest half of the tongue.
These observations led us to believe that in surgery of lingual cancer thorough total hemiglosse- ctomy including the root of the tongue must be performed.

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Speech disorders after hemiglossectomy in ele- ven cases of lingual cancer were studied and the following facts were obtaned.
1.In articulation organs, palatopharyngeal and lingual function were disturbed in all cases.
2.The results of articulation test in pronoun- ciation showed 90% in one case and those under 60 % conld not be found in the remainder.
3.By Sonagraph test, it was revealed that disturbance of vowel was caused by the injury of resonant cavity and that of consonant by the injury of the tongue.
4.Marked differences were noted in Hemiglos- sectomy between the cases where the root was resected and the cases where the root was left intact.
5.Speech function is recoverable by.compen- satory function of the rest of the tongue, which mobilizes its position to the median.posiion.
6.Those who spoke relatively well were past office-clerks who must use speech, or those 10 post- operativeyears.
7.For speech rehabilitation of the glossecto- mized cases, speech therapy was needed.

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Microradiographicstudies in the sialogram of the submandibular gland of the dog in various conditions were carried out and the results are as follows.
Techniqe of X-ray ;500u thick frozen sections of the submandibular gland were put on the films. The pictures were made by means of Softex(18 KV and 10mA).
(1) The normal group:The contrast medium (40% Moljodol with lard added), which filled the Wharton's duct and the branchs of the duct and arrived to the acini showed the small spotty sha- dows.An increase in the amount of the injected medium showed an in crease in density and enlarg- ment of the small spot shadows.
(2) The group with acute inflammation:Ma- ny of the sialograms showed the dapple-like sha- dows at the gland portion.The microsialogram (M-gram) of these cases indicated granule-like or confluenced shadows of medium, which were for- med by leakage of the medium in the tissue of the surrounding of the ductules.
(3) The group with destructive alteration: The sialogram showed the dapple-like shadows and lighter than normal shadows at the gland portion. The M-gram of those cases was like(2), but of these cases the medium at the gland was rarely found.
(4) The group with chronic inflammation: The duct system of the group in the sialogram showed the marked dilatation and the shadows at the grand portion showed almost normal types.
The M-gram of these cases presentad marked dila- tation of the ductules and gave scattered small granule-like shadows at the gland portion.
(5) The group with ligation of the Wharton's duct:The sialogram was characteristic of the dilatation of the duct system.The findings of the M-gram were also remarkable for the dilatation of the duct, and showed unevenly small spot or small granule-like shadows at the gland portion.

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Noise has teratogenic activity on ddN strain mice.The present experiments were carried out for the purpose of elucidating the mechanism of this activity.To accomplish this purpose, the relation of the adrenal function of pregnant animals to the deformities of fetuses was investigated.
Pregnant Wistar strain albino rats were used in this experiments because adrenalectomy was difficult in mice.On the 19th day of pregnancy, all fetuses were taken out, and macroscopically examined to find various malformations.
It was recognized that bilateral adrenalectomy on the 8th day of pregnancy did not produce any macroscopically evident deformities in fetuses if dams were given 0.5mg DOCA by injection and 1% saline as drinking water after operation.As teratogenic procedure in this experiment, noise sti mulation(white noise of 110 phon)and injection of 1% trypan blue solution(1.0cc per 100g body weight) were used concurrently.The noise sti- mulation alone had no effect of producing any malformations, but the noise applied with the in- jection of trypan blue(TB), which was known to act co-operativly with the noise, produced many deformed fetuses, and the incidence of deformities were higher than in the cases of TB injection alone.
The TB solution was injected subcutaneously on the 7th day of pregnancy, and the noise was applied 6 hours daily from the 9the day to the 15th day of pregnancy.When bilateral adrenalectomy was performed at the period between TB injection and noise stimulation, the incidence of deformities was significantly lower than in the non-adrenalectomized animals.When, however, bilateral adrenal medul- lectomy was performed before pregnancy, both the TB injection and the noise stimulation did not cause any significant difference in the incidence of deformities from the control.
From these results, it is clear that the adrenal plays an important role in manifestation of terato- genie activity of the noise, and further that the adrenal cortex plays a more direct role than the medulla.

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