All issues
Volume 96, Issue 1
Displaying 1-18 of 18 articles from this issue
- |<
- <
- 1
- >
- >|
-
SHIROH MAGUCHI, SETSUO TAKAGI, MIKA YOSHIDA, SATOSHI FUKUDA, YUKIO INU ...1993 Volume 96 Issue 1 Pages 1-9,167
Published: January 20, 1993
Released on J-STAGE: October 22, 2010
JOURNAL FREE ACCESSWe reported here 53 cases of birch pollen nasal allergy treated in our department from 1990 to 1991. The 53 patients constituted 16% of 335 total nasal allergy patients. In recent years, the number of birch pollen nasal allergy patients has apparently been gradually increasing in Sapporo.
Skin tests, radioallergosorbent test (RAST) determinations and nasal provocation tests were performed for the diagnosis of birch pollen nasal allergy, and high degrees of correlation were observed among these tests. Therefore, it seems to be reasonable to determine the birch pollen allergy with only a positive case history of early springtime hay fever and RAST determination.
A high correlation coefficient (Rho) value was found with RAST determinations between birch pollen and alder pollen. Furthermore, RAST of alder pollen was significantly inhibited by birch pollen extracts. These results indicate cross reactivity, presumably due to partial immunologic identity between these two pollen allergens.View full abstractDownload PDF (765K) -
HARUO HIRAKAWA, YASUO HARADA, MAMOMU SUZUKI, YOSHINORI KANEMI1993 Volume 96 Issue 1 Pages 10-17,167
Published: January 20, 1993
Released on J-STAGE: October 22, 2010
JOURNAL FREE ACCESSThe effect of calcium ion (Ca2+) in the endolymphatic and/or perilymphatic space of the posterior semicircular canal (p.s.c.) nerve response was studied. When both endolymph and perilymph contained no Ca2+, no p.s.c. nerve response was observed. When only the endolymph contained Ca2+ and the perilymph was Ca2+ free, again no nerve response was observed. On the other hand, when only the perilymph contained Ca2+, p.s.c. nerve response was evoked. Therefore, Ca2+ in the perilymph was considered to be essential for maintaining the function of the p.s.c. as a mechanoreceptor.View full abstractDownload PDF (4758K) -
MASATOSHI HIRAYAMA1993 Volume 96 Issue 1 Pages 18-23,167
Published: January 20, 1993
Released on J-STAGE: October 22, 2010
JOURNAL FREE ACCESSWe investigated the mode of progression of idiopathic bilateral sensorineural hearing loss diagnosed in patients seen in the Hearing Loss Clinic at the Department of Otolaryngology of Kitasato University Hospital. Entered into the study were 105 patients whose courses could be observed for more than 3 years. Audiograms were taken 1069 times in these 105 patients and were examined with regard to the distribution of hearing levels by frequency. Idiopathic bilateral sensorineural hearing loss was divided into three stages from the aspect of the time of hearing change stages I, II and III. Hearing clustering points of the respective stages were compared with each other. Proceeding from the peak of stage I to that of stage II was found to take place at the same peak as that of stage III. Another peak hearing clustering point was noted at s. o..View full abstractDownload PDF (549K) -
TAKASHI FUKAYA, YUKO HATA, ATUSHI KOMATUZAKI1993 Volume 96 Issue 1 Pages 24-28,167
Published: January 20, 1993
Released on J-STAGE: October 22, 2010
JOURNAL FREE ACCESSHearing restoration after removal of cerebellopontine tumors has only infrequently been observed. We present 5 cases with dramatic improvement of both pure tone threshold and speech threshold after excision of tumors. Two patients had acoustic neuroma, 2 meningioma and one cerebellar hemangioblastoma. Preoperatively, the patients suffered severe sensorineural hearing loss and had no measurable speech discrimination score. Recordings of the auditory brain stem response were absent or showed only wave I. However, results of the electrocochleogram were within normal limits. After removal of the tumors, their hearing returned to normal and remained stable thereafter. The data are consistent with the hypothesis that pressure from the tumor causes cochlear nerve conduction block and hearing impairment.View full abstractDownload PDF (454K) -
HIROSHI OGAWA, KAZUHIRO HASHIGUCCI, YUKUMASA KAZUYAMA1993 Volume 96 Issue 1 Pages 29-34,169
Published: January 20, 1993
Released on J-STAGE: October 22, 2010
JOURNAL FREE ACCESSChlamydia pneumoniae has recently been recognized to cause various human respiratory tract diseases, including pharyngitis, bronchitis and pneumonia. The most common pathogens of respiratory tract infections such as Streptococcus pneumoniae and Haemophilus influenzae are frequently present in middle ear effusions. Therefore, it is possible that C. pneumoniae play a role in otitis media with effusion (OME).
Isolation of C. pneumoniae from middle ear aspirates of OME was performed by the culture method using the HeLa 229 or HL cell line. Identification as C. pneumoniae was based on positive staining for inclusions by a species-specific fluorescein-conjugated monoclonal antibody.
C. pneumoniae was recovered from 17.8% (13 of 73) of the patients with acute OME, and 7.1% (3 of 42) of those with chronic OME, Moreover, antibodies to C. pneumoniae were measured by the microimmunofluorescence method in 14 patients who yielded the agent, and all of them had detectable antibodies to C. pneumoniae. Evidence of recent or current infection by the organism was found in nine patients on the basis of either on IgM antibody titer of 1: 16 or higher, or a fourfold rise in the titer of IgG antibody.
The study findings indicate that C. pneumoniae can cause OME and that the organism is a new candidate as an etiological agent of middle ear diseases.View full abstractDownload PDF (536K) -
MORPHOROLOGY AND THEIR MALIGNANCY OF CULTURED COLONIESTAKESHI KUSUNOKI, YASUHIRO YOSHIOKA, YOSIHIKO KOHDA, KIYOTAKA MURATA, ...1993 Volume 96 Issue 1 Pages 35-40,169
Published: January 20, 1993
Released on J-STAGE: October 22, 2010
JOURNAL FREE ACCESSThyroid cells from 14 normal subjects, two patients with Grave's disease, four patients with follicular adenoma and eight patients with papillary carcinoma were cultured in collagen gel. The colonies of these cells were stereoscopically observed and their morphological characteristics were studied with regard to relation with pathological findings of mother tumor, extra-capsular invasion and metastatic potential. For normal thyroid, Grave's disease and follicular adenoma (except for one case), their own characteristic branching type colonies were found. For papillary carcinoma, both branching type and spheroid type of colonies were observed.
The ratio of branching type/spheroid type varied individually in the patients with papillary carcinoma. However, the spheroid type was found to trend to be predominant in patients with extra-capsular invasion and/or lymph node metastasis. This means that the observation of spheroid type colonies in collagen gel is suggestive of risk of extra-capsular invasion or lymph node metastasis.
From the obtained results, it seemed possible to diagnose poorly-differentiated cells in vitro by morphologically observing colonies of human thyroid papillary carcinoma cells developing in collagen gel.View full abstractDownload PDF (4653K) -
MORPHOLOGICAL CHARACTERIZATION AND PROLIFERATIVE ACTIVITIES OF CULTURED CELLS ON FIBROBLAST-REORGANIZED COLLAGEN GELSSACHIO TAKENO, YUKIO HAMAI, TAKAHARU TATSUKAWA, NOBUHIRO HAMAMURA, KAT ...1993 Volume 96 Issue 1 Pages 41-47,169
Published: January 20, 1993
Released on J-STAGE: October 22, 2010
JOURNAL FREE ACCESSLong-term culture of the middle ear epithelium of guinea pigs was carried out on reconstituted floating collagen matrix. Fibroblasts established from the abdominal skin dermis of allogenic animals were used to reorganize hydrated collagen gels into a dermal-like matrix. The explants placed on the surface of these matrices were composed of pseudostratified columnar cells with polygonal flat outgrowth sheets, which could be maintained for up to one month. In contrast, with simple hydrated collagen gel, no lysis of the substrate was observed regarding this reorganized collagen gel.
In order to examine the growth pattern of these culture cells, 5-bromodeoxyuridine (BrdU) was added to the medium during the entire culture period. The specimens were immunohistologically stained using monoclonal antibodies. Noticeable changes were observed in the marginal portion of the explant where the cell shape changed from cuboidal to squamous. In this transitional area, most cells showed positive staining and consequently high proliferative activity. In the central area of the explants, however, the number of positive cells decreased and the main observation was a few labeled basal cell nuclei. It was suggested that the same regenerative process which usually occurs in normal respiratory epithelia after mechanical injury or other insults, was replayed in this culture system.View full abstractDownload PDF (5575K) -
SHOICHIRO TSUTSUMI1993 Volume 96 Issue 1 Pages 48-57,169
Published: January 20, 1993
Released on J-STAGE: October 22, 2010
JOURNAL FREE ACCESSPlummer-Vinson syndrome is a clinical entity associated with dysphagia, sideropenic anemia and atrophic glossitis. Using rabbits with iron deficiency anemia, the author investigated the cause of dysphagia in this syndrome. Iron deficient animals were produced by bolus feeding without iron and intramuscular injection of an iron-chelating agent. The fibers of swallowing muscles (the thyro-pharyngeal, cricopharyngeal and cervicoesophageal muscles) were classified into three types (Type 1, 2A and 2B fibers) by actomyosin ATPase staining. No significant difference between the muscles of sideropenic rabbits and those of normal rabbits were found in the composition and distribution of their muscle fibers. By NADH-TR staining, however, the disturbance of the intermyofibrillar network and/or a “Moth Eaton” appearance, known to be caused by leakage of mitochondria, were observed in Type 1 fibers of the swallowing muscles of sideropenic rabbits. These morphological changes are similar to those observed in progressive muscular dystrophy. The quantity of iron in the swallowing muscles of sideropenic rabbits was significantly reduced in comparison with that in the sternothyroid and femoral muscles. This finding suggests that a selective decrease in myoglobin occurs in the swallowing muscles of iron deficient animals. From these observations, it might be concluded that dysphagia in iron deficiency anemia is caused by a myogenic disorder.View full abstractDownload PDF (7105K) -
YOSHIHIRO ASAI, HIROYUKI MINETA, KAZUO UMEMURA, AKIO YASUHARA, HIROYUK ...1993 Volume 96 Issue 1 Pages 58-65,171
Published: January 20, 1993
Released on J-STAGE: October 22, 2010
JOURNAL FREE ACCESSStatistical analysis was performed on the postoperative recurrence of forty four patients with thyroid papillary adenocarcinoma, who underwent radical surgery in the Department of Otolaryngology, Hamamatsu University Hospital from January 1978 to March 1991. In this study, we defined a new factor called “Operative Radicality index (OR index)” and analyzed the data with a multivariate analysis (Cox model). The result showed that the risk of postoperative recurrence was significantly affected by the age, the size of tumor and the OR index. The postoperative five-year cumulative recurrence rate was approximately 10% in the group of patients whose lateral neck regions were systematically dissected only when any metastatic lymph nodes were clinically detected within the region, in addition to the routine anterior neck dissection, and 70% in the group of patients whose metastatic lymph nodes in the lateral neck region were partially removed, but 6% in the group of NO patients who did not undergo lateral neck dissection because no nodal metastases were detected even in the operative findings. Considering the quality of postoperative life, we concluded that in the primary surgery for the thyroid papillary adenocarcinoma, the lateral neck dissection should be applied to only the regions with matastatic nodes detected preoperatively or during operation, in addition to the routine anterior neck dissection. And the OR index was useful for the statistical analysis with regard to the policies of treatment and the prognosis.View full abstractDownload PDF (712K) -
USING A RETROGRADE TRACER TECHNIQUE WITH CHOLERA TOXINTOSHIYUKI UNO1993 Volume 96 Issue 1 Pages 66-76,171
Published: January 20, 1993
Released on J-STAGE: October 22, 2010
JOURNAL FREE ACCESSThe distribution of the autonomic neurons sending fibers into the canine superior and inferior laryngeal nerves was investigated by an immunohistochemical technique with cholera toxin B subunit as a retrograde tracer.
Cholera toxin was applied to the right internal branch of the superior laryngeal nerve (SLNI), external branch of the superior laryngeal nerve (SLNE) or inferior laryngeal nerve (ILN) in 2-month-old dogs. In every case of cholera toxin application, labeled neurons were seen mainly in the caudal portion of the ipsilateral superior cervical ganglion, whereas none were seen in the stellate ganglion. The number of labeled neurons in the superior cervical ganglion after application of cholera toxin to the SLNI was over 20 times that observed after application to the SLNE or ILN.
When cholera toxin was applied to the SLNI or SLNE, labeled neurons were found mainly in the ipsilateral dorsal motor nucleus of the vagus (DMNV), and a few labeled neurons were found in the ipsilateral reticular formation. The neurons in the DMNV were localized, on the average, between 2.7mm and 5.3mm rastral to the obex. The number of neurons in the DMNV labeled retrogradely from the SLNI was much larger than that from the SLNE. In contrast, no labeled neurons were detected in the DMNV after application to the ILN.
These results demonstrate the following;
1) The sympathetic neurons innervating the canine larynx are distributed mainly in the caudal portion of the superior cervical ganglion and they send their fibers mainly into the SLNI.
2) The parasympathetic neurons innervating the canine larynx have a limited distribution in the dorsal motor nucleus of the vagus and their main pathway is also the SLNI.View full abstractDownload PDF (6826K) -
YOKO IMAMURA1993 Volume 96 Issue 1 Pages 77-87,171
Published: January 20, 1993
Released on J-STAGE: October 22, 2010
JOURNAL FREE ACCESSHuman mucosal microvascular endothelial cells (HMMECs) were isolated and cultured from nasal inferior turbinates. These cells can be maintained for up to 30 passages over 10 months, as long as human umbilical vein endothelial cells (HUVECs), using dish-coated collagen and MCDB 107 medium supplemented with 10% fetal bovine serum and 75μg/ml of crude endothelial cell growth factor prepared from bovine brain. These cells readily formed capillary-like structures when cultivated on dish-coated collagen or collagen gels as compared with HUVECs. The collagenase activities of conditioned medium from HMMEC culture on collagen gels were considerably higher in tumor conditioned medium while those from HUVECs were undetectable. The capillary-like structure in the collagen gel and the collagenase activities of conditioned medium were periodically changed during long-term cultivation of HMMECs. A good correlation between the two was obsersed.
These results, suggest that there are phenotypic differences between HMMECs and HUVECs and that collagenase plays an important role in angiogenesis. These cultured HMMECs could serve as a valuable experimental model for determining mucosal pathogenesis in the nasal and paranasal sinus regions.View full abstractDownload PDF (8776K) -
IMMUNOHISTOLOGICAL STUDYTAKAO SAMUKAWA1993 Volume 96 Issue 1 Pages 88-97_1,171
Published: January 20, 1993
Released on J-STAGE: October 22, 2010
JOURNAL FREE ACCESSWe investigated the expression of HLA-DR antigen on 42 squamous cell carcinomas of head and neck using immunohistological methods. Our results indicate significant relationships between HLA-DR antigen expression on carcinomas and lymphocytic infiltration, that is, CD8 positive lymphocytes (CD8/CD3) infiltrated HLA-DR antigen positive carcinomas while CD4 positive cells (CD4/CD3) were found in HLA-DR negative carcinoma. The Ki-67 (a nuclear marker of proliferation) positive rate was lower on HLA-DR positive carcinomas than on negative carcinomas. However, there was no correlation with pathological differentiation, clinical stage or primary lesion. In vitro studies using several cytokines showed that IFN-γ induced expression of HLA-DR antigen and lowered the Ki-67 positive rate on squamous cell carcinomas. It seems that HLA-DR antigen expression on squamous cell carcinomas could be induced by cytokines released from the infiltrating lymphocytes.View full abstractDownload PDF (1741K) -
KENJI MACHIKI, AKIRA HARA, JUN KUSAKARI, ISOLDE THALMANN, RUEDIGER THA ...1993 Volume 96 Issue 1 Pages 98-106,173
Published: January 20, 1993
Released on J-STAGE: October 22, 2010
JOURNAL FREE ACCESSThe tectorial membrane is an acellular connective tissue which plays an essential role in cochlear function. While a comparatively large amount of information is available on the collagen network of the tectorial membrane, studies on the biochemical nature of this highly hydrated matrix, which is composed of proteoglycans (PGs) and glycosaminoglycans (GAGs), have been quite limited.
Previous reports on the biochemical analysis of the tectorial membrane have failed to detect uronic acid, which is present in large amounts in all mammalian GAGs except keratan sulfate. Applying a colorimetric assay based on the binding of GAGs to cationic dye Safranin-0 in combination with enzymatic techniques, we were able to measure GAGs in the murine tectorial membrane. Approximately 0.3% uronic acid-containing GAGs (mainly in the form of chondroitin/dermatan sulfate) and 0.17% keratan sulfate were detected in the tectorial membrane (both on a wet weight basis). In addition, various types of electrophoresis revealed one large PG with a molecular mass similar to that of the large type cartilage PGs and three small PGs, containing chondroitin sulfate and keratan sulfate side chains, respectively.
Judging by coelution of standards, one of the small PGs seemed to correspond to fibromodulin, which has at least one keratan sulfate side chain, and binds to type I and type II collagen to regulate collagen organization in tissues. Our results suggest: (1) Donnan equilibrium is established in the tectorial membrane because sulfated GAGs are highly negatively charged and consequently bring about an influx of large amounts of water and cations into the matrix. (2) The tectorial membrane is highly organized by collagen as well as large and small PGs, especially in the direction in which major mechanical force is exerted. Therefore, the tectorial membrane is assumed to maintain the exact alignment of insertion of tips of stereocillia so that they are not bent in the resting state.View full abstractDownload PDF (4469K) -
MASAKI FURUKAWA, MADOKA FURUKAWA, MAMORU TSUKUDA, KOHTAROU YAMASHITA, ...1993 Volume 96 Issue 1 Pages 107-114,173
Published: January 20, 1993
Released on J-STAGE: October 22, 2010
JOURNAL FREE ACCESSAlthough fine needle aspiration cytology (FNA) is widely used in the otolaryngology, few hospitals perform it in conjunction with ultrasonographic imaging. Despite the benefits of ultrasonographic imaging-assisted FNA, it is not widely utilized due to the prevalent misperception that the procedure is exceedingly complicated.
In an effort to refine FNA procedures under ultrasonographic imaging (focusing on safety and simplification), we conducted a study on outpatients with neck tumors. We developed the following guidelines.
1. The aspiration was conducted free-hand without the use of mechanical devices such as special probes and/or aspiration adaptors.
2. Positioning of the probe was adjusted so that the puncture site was centered in the image.
3. When the tumor was located near the carotid artery or jugular vein, the probe was adjusted so that both the tumor and the blood vessel could be observed in the same image.
4. The needle was introduced at the center of the probe from the sliced direction. The needle injection route was then adjusted in line with phase crossing at right angles with the scanning field of the probe.
5. The depth of the puncture was adjusted in accordance with the angles between the probe and the needle.
From June 1985 to December 1990, FNA under ultrasonographic imaging was conducted on 149 patients. It was possible to compare both cytological and histological studies. According to site, lymph nodes ranked highest in 52 cases, followed by parotid glands (45) and thyroid glands (27).
A 5 or 7.5 MHz probe was used with 21- or 22-gauge injection needles. Of the 93 cases which were diagnosed as class I, II or IIIa, based on cytological examination, 83 (89%) were shown to be benign. Among the 56 patients diagnosed as class IIIb, IV or V, 49 cases (88%) were malignant. The overall accuracy rate was 89%.
No complications occured in the present studies and our method of ultrasonographic imagingassisted FNA proved to be a simple and safe diagnostic procedure.View full abstractDownload PDF (7352K) -
1993 Volume 96 Issue 1 Pages 115-128
Published: January 20, 1993
Released on J-STAGE: December 22, 2010
JOURNAL FREE ACCESSDownload PDF (1435K) -
1993 Volume 96 Issue 1 Pages 129-139
Published: January 20, 1993
Released on J-STAGE: December 22, 2010
JOURNAL FREE ACCESSDownload PDF (1126K) -
1993 Volume 96 Issue 1 Pages 139-150
Published: January 20, 1993
Released on J-STAGE: December 22, 2010
JOURNAL FREE ACCESSDownload PDF (1142K) -
[in Japanese], [in Japanese]1993 Volume 96 Issue 1 Pages 152-155
Published: January 20, 1993
Released on J-STAGE: October 22, 2010
JOURNAL FREE ACCESSDownload PDF (1019K)
- |<
- <
- 1
- >
- >|