High virulent RH strain and the lower virulent Beverley strain of Toxoplasma gondii were used in this experiment. RH strain kills all the mice inoculated within a week while Beverley strain often produces chronic infections in mice producing cysts in their brains.
1) When Beverley strain was inoculated into mice, proliferative forms first appear in the abdominal cavity 3 or 4 days after inoculation and the highest population was usually attained 7-11 days after inoculation. These proliferative forms diminished in number gradually thereafter. The longest duration of the presence was 18 days in one case. No essential difference was recognized between the normal and splenectomized mice in the period of appearance of the proliferative forms in the peritoneal fluid.
2) Shape and size of these proliferative forms showed no essential difference with those of the typical RH strain, only the breadth of the former being a little larger than the latter.
3) After the disappearance of the proliferative forms from the peritoneal fluid by the direct microscopical examinations, the peritoneal fluid of these mice was drawn repeatedly with different intervals and inoculated into clean mice. By these animal inoculations the peritoneal fluid proved to be infective until 33 days after inoculation. In one exceptional case the fluid drawn 78 days after inoculation produced cysts in mice inoculated. After the disappearance of the typical proliferative forms from these peritoneal fluid, smaller Toxoplasma-like bodies were often found by the microscopical examination. These small bodies may be the agent which produced infection in mice inoculated.
4) Mice inoculated with RH strain of Toxoplasma were treated with pyridazine (3-Sulfanilamido-6-methoxypyridazine). Survival period of these mice was prolonged: 38 of 142 mice survived up to 3 weeks or more after inoculation. Proliferative forms appeared in the peritoneal fluid 2 days after inoculation and disappeared mostly within 11 days, with an exceptional case in which they were first seen on the 11th day and disappeared on the 15th day.
5) These proliferative forms found in the pyridazine-treated mice were much larger in breadth than the normal proliferative forms of the same strain.
6) Mice which survived the RH infection for more than 20 days were examined for the presence of cyst. Among 26 of these mice 13 were found to have had cysts in their brains. Brain emulsions of these mice, regardless whether they were positive or negative of cysts by the microscopical exami-nations, all produced acute infections when inoculated into clean mice. All of these mice succumbed to the acute infection.

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A study of the aerobic and anaerobic phase of glucose metabolism in rabbit retina was undertaken by analysis of the relationship between E.R.G. and corneal pulse waves utilizing of synchronous recording them on the acute ishemia of chorioretinal circulation giving pressure to the eye-ball.
Studies have shown that the maintenance of the glucose level in the retina may be a factor related to b-wave generation, and the insulin appears to play a prominent hypoglycaemic action in the retina.
The insulin effect on the initial increasing of retinal anaerobic metabolism in this experiment could also be considered as a acceleration for glucose transfer mechanism across the blood-retinal barrier. The insulin effect on the negative decreasing of retinal anaerobic metabolism 30 min to 60 min following adminis-tration in this experiment may be considered as a promotive action of anoxia for insulin. On the contrary Diamox may be counter active for retinal metabolism compared with insulin, and it is suggested that Diamox change the peripheral transfer mechanism of glucose to be opposed to insulin effect.

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Symptoms of swelling of the right cheek and exophthalmos of the right eye were complained of in an infant, aged 1 year and 6 months. With a diagnosis of maxillary sinusitis the infant was operated upon. Microscopic examination of the removed tissue was reported as undiffentiated cell cancer. The patient was treated with radiation therapy, but died about 6 months after the operation. Postmortem examination of the tissue proved the affection to be a multiple myeloma.
We are grateful to be instruction and assistance of Prof. Toshikazu Nishihata, chief of the clinic of Oto-rhino-laryngology, Prof. Keizo Kageyama and Kenjiro Arakawa, M. D., at the department of Pathology.
This article is released on Feb. 22 in 1959, at 359th Session of Oto-rhino-laryngology in Kanto District, and on June 21 in 1959 at the Societe d'oto-rhino-laryngologie de Bordeaux et du Sud-ouest (Saint-Sebastien).

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When timothy seeds which had previously been soaked in streptomycin solution for 4 days at 5°C were rinsed in metal salt solutions or in water whose pH value was adjusted to 3.5 by adding diluted HCl for 24 hours at 5°C, antibacterial activity could be detected against E. coli in the rinsing solutions. This antibacterial activity may be accounted for the streptomycin which has been driven out of the streptomycin-treated seeds. However, when the streptomycin-treated seeds were rinsed in pure water or in water whose pH value was adjusted to 10.5 by adding diluted NaOH, the streptomycin activity was negative in them. These facts will be conductive to the explanation of why the streptomycin-treated seeds when they are rinsed with various metal salt solutions or with water of lower pH value can sprout chlorophyll-recovered leaves on the cotton moistened with pure water, and why the streptomycin-treated seeds cannot produce chlorophyll-recovered leaves when they are rinsed with pure water or with water of higher pH value which contains only a minute amount of diluted NaOH.
Seasonal variation was observed in the potency to recover chlorophyll in the leaves, that is, recovery was animated during spring-to-early summer period and also in autumn, while it fell down markedly in winter. However, the effect of metal salt to drive the streptomycin out of the seeds with which streptomycin was combined was found almost the same in winter when the recovery potency of the seeds declined as in spring when the potency was animated. Therefore, it seems that in order to actualize this chlorophyll recovery it may be required for the seeds themselves to have enough vitality to make active the motivity of the system governing chlorophyll formation, although the driving of streptomycin out of the seeds with which streptomycin is combined should be the most influential cause for this chlorophyll recovery.
The author is indebted to Dr. Hideo Moriyama, the director of this institute, for his many valuable advices and encouragements, and my thanks are also due to Mr. Hiroshi Ueno of this institute for his kindly cooperation in bacteriological technique.

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