巻号一覧
39 巻, 1 号
選択された号の論文の5件中1～5を表示しています
- |<
- <
- 1
- >
- >|
-
Howard E. Morgan1990 年 39 巻 1 号 p. 1-5
発行日: 1990年
公開日: 2009/03/27
ジャーナル フリーTransduction of stretch of the ventricular wall into accelerated growth and ultimately hypertrophy of cardiac muscle cells is a cyclic AMP (cAMP) dependent phenomenon. When stretch was induced in isolated perfused rat hearts by an increase in aortic pressure from 60 to 120 mmHg, protein synthesis was accelerated during the second hour of perfusion. Only a brief exposure to higher aortic pressure (2 min) was required to elicit this effect. Elevation of aortic pressure also increased cAMP content. Other interventions that increased cAMP content such as glucagon increased second hour rates of protein synthesis. Stretch of the ventricular wall had a more rapid effect on ribosome formation. During the first hour of perfusion, increased aortic pressure raised rates of 60S ribosomal subunit formation by 38% in the absence of added insulin and 35% in the presence of the hormone. Ribosome formation was also accelerated by addition of glucagon. The muscarinic cholinergic agonist, methacholine blocked the effects of elevated aortic pressure on protein synthesis, ribosome formation, and cAMP content. These studies indicate that stretch of the ventricular wall is transduced into greater cAMP content and that this intracellular messenger is one of the substances responsible for accelerated ribosome formation and protein synthesis.抄録全体を表示PDF形式でダウンロード (376K) -
上 敏明1990 年 39 巻 1 号 p. 6-13
発行日: 1990年
公開日: 2009/03/27
ジャーナル フリーThis study was designed to prove that survival of flaps was improved by the hypobaric oxygen training before making flaps. Wister strain male rats weighing 200-250 grams were used as the experimental animals. Rats were kept in a hypobaric simulator two hours a day for two weeks, which was set to emulate a condition of 8000 meters above sea level (hypobaric oxygen training). When the hypobaric oxygen training was finished, flaps were elevated on the back of the rats. Survival rate of flaps were significantly increased at 7th post-operative day. But its effect has been diminished until the 3rd training day. The mechanism of hypobaric oxygen training was studied by blood cell count and measurement of serum prostglandin E level, micro-angiogram of the flap and the count of insulin particle in the dermis. Consequently, it is postulated that the mechanism of hypobaric oxygen training was not morphological and functional change in the flap vascularity but the cellular metabolic adaptation to the hypobaric state.抄録全体を表示PDF形式でダウンロード (3040K) -
小川 Ｍ．マリリア, 橋本 隆, 鈴木 達夫, 西川 武二1990 年 39 巻 1 号 p. 14-20
発行日: 1990年
公開日: 2009/03/27
ジャーナル フリーAn optimum culture condition was established for our keratinocyte cell culture system from human adult skin using 3T3 feeder cells. Calcium ion (Ca++) concentration was found to be critical and cells grew best at the Ca++ concentration of 0.2 mM. Keratinocyte proliferation was promoted when 0.4 μg/ml hydrocortisone and 7 μg/ml insulin were added. However, epidermal growth factor, cholera toxin and transferrin did not show stimulating effects on multiplication of human keratinocytes in our culture system. The epidermal sheets grown in vitro under this optimized condition were transplanted onto athymic mice, and a short term of take was observed.抄録全体を表示PDF形式でダウンロード (1144K) -
村木 淳郎1990 年 39 巻 1 号 p. 21-25
発行日: 1990年
公開日: 2009/03/27
ジャーナル フリーSynergistic effect of recombinant IFN-gamma (IFN-γ) and OK-432, a streptococcal preparation, using chromium release assay was studied in vitro on killer cell induction. The target cells utilized for assay were a human leukemia cell line K562, a human renal carcinoma cell line KU-2, autologous normal kidney tissues and autologous renal cell carcinomas. Culture supernatant of peripheral blood lymphocytes (PBL) and OK-432 (designated as OK conditioned medium or OK-CM) demonstrated enhanced cytotoxicity of fresh PBL against these target cells. Killer cell activity against autologous cancer cells could be also induced from PBL of renal cell carcinoma patients. Pretreatment of PBL with IFN-γ revealed synergistic effect of OK-CM on killer cell induction. OK-CM derived from patients was shown to contain IL-2 activity as well as high titer of interferon. Neutralizing monoclonal antibody against IFN-γ and IL-2 receptor demonstrated reduction of cytotoxicity. These results suggested potential benefit of sequential use of IFN-γ and OK-432 for the treatment of metastatic renal cell carcinoma.抄録全体を表示PDF形式でダウンロード (2026K) -
長沼 裕一郎1990 年 39 巻 1 号 p. 26-31
発行日: 1990年
公開日: 2009/03/27
ジャーナル フリーIn cerebral ischemia, ultrastructural changes of the microvessels were investigated in relation to the further development of microcirculatory disturbances. Experimental ischemia was induced by occlusion of the unilateral common carotid artery (CCA) in mongolian gerbils. The animals were sacrificed at set times ranging from 6 to 96 hours after the occlusion. After 6 hours of ischemia, swelling of perivascular astrocytic processes was seen, although there were no obvious changes in the endothelium. After 12 hours, perivascular glial swelling became more prominent and narrowing of the microvascular lumen was observed. After 24 or 48 hours, an irregular luminal surface with endothelial projections was noted. These changes may have had some influence on the brain microcirculation. At 96 hours of ischemia, the cytoplasm of the endothelium appeared granular and thrombus formation was occasionally observed on the exposed basement membrane of those microvessels whose endothelium had been exfoliated. Thus, in the permanent ischemia of the present study, exfoliation of endothelium was thought to have caused the thrombus formation, promoting further microcirculatory disturbance and consequent progress of the brain damage.抄録全体を表示PDF形式でダウンロード (2309K)
- |<
- <
- 1
- >
- >|