1. The deletion of any ingredients from cystein-bouillon-serum medium did not support the growth of
T. vaginalis.
2. The trichomonad showed active multiplication in the medium added with 0.3 to 0.7% of glucose.
3. The growth of the organism is markedly stimulated by addition of glucose, fructose, maltose, glycogen and dextrin, and considerably stimulated by sucrose and soluble starch, while lactose and galactose gave no stimulations. It should be noted that glycogen was directly utilized resulting in stimulation. of the multiplication.
4. Consumption of glucose, shift of pH toward acid side and accumulation of lactic acid were recognized in the course of multiplication of
T. vaginalis in vitro. The amount of glucose consumed by the multiplying organisms from 9, 000 to 3, 000, 000 individuals was 4.5mg. The lactic acid was produced in the concentrations of about 20 mg per dl during 96 hours incubation.
5.
T. vaginalis has succinic-, malic-, and citric-dehydrogenase indicating the probale existence of TCA cycle in the course of its metabolism.
6. The dehydrogenase activities were strictly inhibited by monoiodoacetate and, cyanide and malonate had no effect upon the activities.
7. Under the condition tested,
T. vaginalis consumed much oxygen comparing with.
T. foetus and
T. gallinae. The optimum pH for oxygen uptake of three species of trichomonads agreeded with that of growth in the medium.
8. A low catalase activity was recognized in
T. vaginalis, while
T. foetus and
T. gallinae showed high activities.
Summaries of this paper were read at the Anual Meeting of Japanese Parasitologists in 1952, 1953, 1954 and 1956.
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