It is known that lactic anions and hydrogen ions (H
+) produced during intense exercise are partly transported or diffused from muscle to blood resulting in the production of non-metabolic CO
2 through the bicarbonate buffering system. The purpose of the present study was to examine the reliability of the estimation of non-metabolic CO
2 output using respiratory gas analysis during incremental exercise. Six healthy subjects underwent an incremental pedaling exercise test accompanied by respiratory gas and arterial blood sampling. The rate of non-metabolic CO
2 output (VCO
2-NM) was calculated by subtracting projected metabolic VCO
2 from actual VCO
2 after CO
2 threshold (CT) . CT was determined using a modified V-Slope method. Bicarbonate (HCO
3-), pH, CO
2 partial pressure and lactate concentration were measured from arterial blood samples using automatic analyzers. The kinetics of VCO
2-NM and HCO
2- were compared throughout the exercise test. VCO
2-NM was significantly correlated with HCO
3-decrease after CT (r=0.976, p<0.001) and the kinetics of VCO
2-NM and HCO
3- decrease were similar during exercise. Furthermore, the amount of non-metabolic CO
2 output (NM-CO
2) calculated integrating VCO
2-NM above CT was significantly correlated with the difference in HCO
3-between CT and exhaustion (r=0.929, p<0.01) and with the difference in arterial blood pH between rest and exhaustion (r=0.863, p<0.05) . However, NM-CO
2 was not significantly related to maximum ventilation (r=0.111, ns) . These results suggest that the estimation of non-metabolic CO
2 output during incremental exercise proposed in the present study is reliable. It was also suggested that the primary factor which influenced nonmetabolic CO
2 output during incremental exercise was the addition of H
+ into blood and not hyperventilation.
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