A dental health survey was conducted on the population of Hongo-mura lacking any facility for dental care, and the results obtained were summarized as follows. 1. Socio-economic status : In spite of relatively poor economic status of the community as compared with the nation-wide average and the resulting lack of dental health service for the inhabitants, the dental hygienic status proved not so bad as may be anticipated. 2. Deciduous teeth shedding : Shedding occurred earlier in the female than in the male in Hongo-mura inhabitants. However, shedding in this population occurred later than in the comparable population of Tokuyama city, earlier than in Tonoi-mura population, and approximately at the same age stages with Yoshikawa-mura population. 3. Eruption of permanent teeth : Eruption of permanent teeth occurred earlier in the female than in the male. However, with both sexes the eruption was retarded in this population than in Tokuyama population, but accerelated than in Tonoi-mura and Yoshikawa-mura populations. 4. Average number of d.f.t. : There was found no significant difference between both sexes of the population in this respect. The number of d.f.t. in the population, however, was greater than that in Tokuyama population and smaller than those in Tonoi-mura and Yoshikawa-mura populations. 5. Average number of D.T. : The female had greater number of D.T than the male in this population. Comparison revealed no significant difference in the number of D.T between the present population and those of Isshakuya village and Shimonoseki city. 6. Average number of M.T. : There was no significant sex difference, nor was there any difference between the present population and that of Isshakuya village in this respect. Shimonoseki population had more missing teeth than the present population. 7. Average number of F.T. : The female member had more filled teeth than the male. However the present population had less F.T. as compared with Isshakuya and Shimonoseki populations. 8. Average number of D.M.F.T. : The female had more D.M.F.T. than the male of the present population, but the latter had less D.M.F.T. as compared with Tokuyama city, Tonoi-mura, Yoshikawa-machi, Isshakuya village and Shimonoseki city populations. 9. Ratio of Σ.F.T. versus Σ.D.M.F.T. : There was no sex difference in the ratio. However, the ratio with the present population including both sexes was lower than those with Isshakuya village and Shimonoseki city populations. 10. Average D.M.F.T. index : The female showed higher value than the male. However, the index for the present population including both sexes was lower than that for Yoshikawa-machi population, and approximately the same with that for Isshakuya village and Shimonoseki populations. It was concluded that the tooth health status of Hongo-mura population was excellent as compared with Tokuyama city, Tomoi-mura and Yoshikawa-machi populations, but slightly inferior than that of Shimonoseki city and Isshaknya village populations.

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Thiamine and pantothenic acid contents in the human saliva directly obtained from the duct of stimulated parotid glands were assayed by the microbiological method. The amount of thiamine in conjugated form ranged from 0.8 to 8.0 mγ per ml. of the saliva and not a trace of thiamine in the free form existed there. The total amount of pantothenic acid in the saliva ranged from 33 to 101 mγ per ml. of which 16 to 35 mγ were accounted for by the free acid.

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The present report gives warning that uncleanliness of the mouth in general, and especially neglect of decayed teeth, tooth stumps and chronicdental diseases might constitute a factor or factors initiating moniliasis in oral region. In the present case, a 25-year-old female, there was an upper left wisdom tooth decayed to the state of stump which gave constant irritation to the neighboring buccal mucous membrance. This part of the mucous membrane may have lost its normal resistance and gave way to the entrance and local establishment of moniliasis.

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Oral examination and lactobacillus counts were conducted on a selected group of one hundred school children, and the results obtained were summarized as follows. 1. In 58.3 to 60.2 percents of subjects with carious teeth there were found far higher lactobacillus counts than in subjects with healthy teeth. The mean lactobacillus count in the mouth of the former amounted as much as 3-fold that in the mouth of the latter. 2. There was hardly found any significant correlation between the number of decayed teeth and lactobacillus count. However, the mean lactobacillus counts proved to be generally elevated in subjects having high caries incidence. 3. The mean lactobacillus count in the regular users of tooth-brush proved to be lower than that in non-users.

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Lactobacillus count in saliva of pregnant women were examined in relation to dental caries incidence during gestation. Conclusions reached were summarized as follows. 1. Lactobacillus counts were conducted on one hundred pregnant women and 60 control women. Culture medium used was ′Tomato-Peptone-Agar′ (Eiken) supplemented with Natrium azide. 2. The mean number of laetobacillus colonies in the saliva of pregnant women was 599 in contrast to 241 in the saliva of control women which meant approximately 2.5fold. 3. Lactobacillus count in the saliva of pregnant women reached the highest level in the 4th month followed by the 2nd and 3rd during gestation. 4. The rate of caries incidence among pregnant women was 3.4 percent on the average. Although the rate increased toward the end of gestation, it was not accompanied by any parallel increase of lactobacillus count in the mouth. 5. No significant difference in lactobacillus count was found between multiparous and primiparous subjects. 6. Comparison made between groups of different age stages disclosed that older groups rather than younger groups had much higher lactobacillus count. 7. Comparison between subjects having decayed teeth and those having healthy teeth disclosed so far unsuspected result that lactobacillus count in the former was rather low as contrasted with that in the latter subjects.

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