We developed a delayed luminescence (DL) imaging system by employing a fast EMCCD camera. Light from a metal halide lamp can be exposed to specimens in a dark chamber through an optical fiber in the system. Three synchronized mechanical shutters were installed to prevent any direct light or fluorescence of other materials except the specimen from entering the CCD camera. For an application, the delayed luminescence from leaves of Euonymus japonica was imaged as a function of light illumination time. Our study might be applied to study the nature of living organisms in addition to plant physiology on the aspects of biophoton.

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本研究の目的は西野流呼吸法における微小循環応答、免疫活性及びストレスレベルに対する影響について検討しようとするものである。西野流呼吸法は2つの基本的構成からなり、その1つは足芯呼吸であり、他の1つは対気である。微小循環応答は、指尖容積微分脈波(differential digital photoplethysmography; ΔDPG)を用いて位置負荷及びイメージ負荷時に測定した。対気において西野流呼吸法熟練者の心拍数は、初心者と比較して有意に増加した(p<0.05)。NK細胞活性は21名中17名が呼吸法終了後に活性が増大し、全被験者の平均NK細胞活性は有意に増大した(p<0.05)。ストレスレベルは呼吸法終了後に明らかに減少した(p<0.05)。リラックスイメージにおいて、西野流呼吸法インストラクターのΔDPG-P波高増大が観察された。以上の結果より、西野流呼吸法は細動脈機能や免疫活性システムを増強し、さらにストレスを減少させることが明らかになった。西野流呼吸法を継続的に行うことにより、健康がより助長されるものと思われる。

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We developed a novel method to observe the released threadlike structure spread on rat internal organs. The technique we used is to spray hematoxylin on internal organs to stain nuclei of the threadlike structure. We scrutinized the surface of internal organs under stereomicroscope to find the novel structure. Confocal microscope images revealed the distinctive characteristic pattern of nuclei distribution that was consistent with the Bonghan's circulatory network.

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Unique duct system believed to be Bonghan duct and the corpuscle like structures were found on the surface of the liver from rabbit. Diameter of the duct was about 80 urn and formed of a bundle of tubules whose diameter was about 10-15 μm. Inside each tubules small granules of which the size is about 1 um could be directly observed. As the granules form very narrow lines inside the tubules, it is possible that there exist another level of tubular structures through which the small granules flow. The granules were also observed to come out from the surface of duct. Stained section of the corpuscle-like structures showed that they were full of blood cells, suggesting hematopoiesis.

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We have isolated various types of Bonghan ducts. Mostly they consist of a bundle of tubules with fiber-like striae. Bonghan duct isolated from peritonea shows the presence of very large rod shaped cells whose length is almost 50um inside the tubules. Bonghan duct isolated from human umbilical cord also shows the presence large cells at the end section of the tublules, which are of more round shapes. However, sometimes very narrow lines of small granules whose size is about lum were also observed from various types of Bonghan ducts. The electron microscopic examination(TEM) could confirm the existence of the small granules inside the striae forming the tubule, suggesting the striae might be also unique tubular structures. TEM showed that the granules inside striae have the nucleus and the membrane, and even divide. Present investigation reveals that there exist different levels of tubular structures inside Bonghan duct, and further suggests the granules and the cells inside each tubular structures might be intercon verted.

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Threadlike structures that moved freely and were sparsely fixed on the surfaces of various lobes of rat livers were observed. These structures were morphologically in accordance with Bonghan Kim's descriptions about the organ-surface Bonghan ducts. The ducts were transparent, and the thickness varied from 30 to 60μm. There were also corpuscles connected by the ducts and the ducts often divided into two or more branches which were the characteristic features of Bonghan network on the surface of internal organs. By Feulgen reaction we found that streams of Bonghan granules flowed through the Bonghan duct. Thus the bundle structure of the Bonghan duct was observed.

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We report on the observation of a novel structure inside of the blood vessels in rats. This is a rediscovery of the Bonghan ducts, which have not been yet confirmed because the observing method was not known. We applied a new procedure for observing these intra-blood vessel structures. The method we contrived is to let blood be coagulated around them so that they become thick and strong by intravenous injection of dextrose solution into a femoral vein. A piece of thickened sample is treated with urokinase to remove fibrin and blood clots and to recover the thin thread. Histological study was done with hematoxylin and eosin, Mallory's triple and Verhoeff's elastic stains. This intra-vascular thread was a semi-transparent and elastic structure whose diameters were of about 20 -100μm and also had many myoid cell-like rod-shape nuclei.

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Today a great deal of interest is being expressed in the subject of spirituality on health. Spiritual healing is a fascinating subject. Today articles on this topic are appearing in newspapers, magazines and in medical journals. It is being accepted as a practical, demonstrable method of healing for all problems, including diseases. The reasons are many. Perhaps traditional, western, allopathic medicine does not provide answers for many conditions. It is very expensive, sometimes uncomfortable, even painful. Several people turn to homeopathy, herbal medicine, yoga, acupuncture, and various other alternative methods of healing. Examples are given of the healing of flu, back pain, skin rash, and of how the process of spiritual reasoning.

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We used a fluxgate to measure the bio-magnetic field from the palm during Qi emission. Four subjects among ten Qi-gong practitioners showed moderate bio-magnetism of 1nT order. Qi-gong practitioners' bio-magnetism signals exhibit certain characteristic frequencies depending upon each individual. We could not, however, determine whether the observed signal is the bio-magnetic field emitted from the palm or only manifestation of the modulated background magnetic field.

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本研究は特殊な能力を持つ被験者の身体周辺に観測される磁気の分布について報告する。被検者は56才の日本人男性で、クライアントの手を軽く触るだけでクライアントの健康状態を診断することができると主張する。この診断の際に被験者の周辺に発生する磁気を測定した。磁気センサーはフラックスゲート式磁気センサーを用い、1ユニットには直交3軸方向に1個のセンサーを配置させ、磁気の大きさと方向を測れるようにした。また、磁気センサーユニットを10個準備し、各ユニットを被検者の正面から背中側へ水平直線状に配置して人体周辺の磁気分布を測定し分析を行った。分析の結果、どのユニットも磁気の方向は正面から背中へ水平方向に向いており、その強さは被検者の身体に近い背中側が最大であった(8×10^3nT)。また、磁気分布は身体から離れるに従い小さくなるイベントがほとんどであったが、被検者の意識が異なる状態と思われるイベントでは磁気のピーク値(磁界の強さの絶対値)が背中から23cm離れた空間に現れ磁気の分布状態が異なっていた。これらは意識による磁気発生に関する新しい知見を示唆している。

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筆者が13年前に全結腸型の潰瘍性大腸炎の診断を受けて以来、様々な代替医療の臨床応用とその報告を少しずつではあるが行ってきている。当学会にも気功の分野での報告、エネルギー照射水等の報告も行ってきた。今回、低分子化したフコイダンを中心とした代替医療による20代HPV感染早期子宮癌改善例、50代乳癌改善例、40代IV期乳癌、骨転移、肺転移の免疫力上昇、症状改善例、80代男性腎癌の血尿等の症状改善例、70代女性肝癌腫瘍マーカー改善例についての画像、リンパ球データ等の臨床データを含めた報告を行う。

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Corydalis Tuber has traditionally been used for the treatment of pain and arthritis. The effect of Corydalis Tuber against lipopolysaccharide-induced inflammation was investigated using reverse transcription-polynierase chain reaction (RT-PCR), nitric oxide detection, and prostaglandin E_2 (PGE_2) immunoassay on murine macrophage RAW 264.7 cells. The aqueous extract of Corydalis Tuber suppressed nitric oxide production and PGE_2 synthesis by inhibition on the lipopolysaccharide-stimulated enhancement of the inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and interlukin-lbeta (IL-1β) mRNAs expressions in RAW 264.7 macrophages. These results suggest that Atractylodesjaponica exerts anti-inflammatory and analgesic effects probably by suppression of the iNOS, COX-2, and IL-1β expressions.

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FAP (不安からの解放プログラム)という新しい心理療法につき報告した。FAPは実際の臨床において一定の有効性を示し、それは統計的にも確認された。手指を用いる技法としてのFAP診断の理論的な解釈のために、著者は「ミラーニューロン」システムを重視した。マカクザルの脳地図上において、F5領域にある「ミラーニューロン」は運動野や、中心溝を越えて体性感覚野とも直近している。両野においては「手の指」の領域が比較的広い部分を占めている。この状況において、著者は、F5ニューロンにおいて発火したインパルスを運動野および体性感覚野に伝導する経路の存在を想定し、それを「FAP神経路」と名づけた。すなわち、「ミラーニューロン」および「FAP神経路」は、FAPの主要な概念である受療者と治療者との間の「非言語的コミュニケーション」が「手の指」により行なわれることの可能性に寄与するものと思われる。

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One of the Korean traditional medicines, Sa-Sang classifies one's constitution into 4 types, which are referred to as Tae-Yang-In, Tae-Eum-In, So-Yang-In and So-Eum-In. This study was to examine demographic factors, lifestyle, biochemical indicators, and the dietary habit and to test any significant differences between Sa-Sang constitution and related factors. The participants were 483 college students and the data of 428 students were finally analyzed, whose constitution was determined identically through both the questionnaire on Sa-Sang constitution and a Korean traditional medical doctor's inquiry. Tae-Eum-In showed a significant difference statistically in BMI and a higher smoking rate. So-Yang-In is more active and So-Eum-In is more passive during work and in leisure time. So-Eum-In had a tendency to take vegetables and fruits more often. When a degree of roasting meat was indicated, So-Yang-In showed a higher score. Tae-Eum-In showed that his/her propensity to eat a visible fat portion as it is was higher. Considering the potential that most chronic degenerative diseases are developed from any of lifestyle factors, it is necessary to conduct an educational program on lifestyle including the dietary habit. So, it will be of interest to conduct a long-term follow-up study on those groups with any chronic degenerative disease in the future based on the classification of Sa-Sang constitution.

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本研究では,脳波という生体反応を用いて,不快音による人体への影響の定量化を試みた.特に個人差,再現性,脳波自体の定量性を検討した.個人差に関して,音呈示時の計測脳波の解析結果から,音環境に対して不快感を抱くのは,単一の周波数成分が影響するのではなく,複数の音の組み合わせによる影響が原因している可能性を示唆することができた.再現性に関して,消防車のサイレン音,ホワイトノイズはα波が有意に減少し,快音との有意差も認められたため,生体にストレスを与えることが示された.また,同計測による1週間後の脳波データ,同一日の連続計測による3回までの脳波データは信頼性が高いことが示された.脳波の定量性に関して,同時計測した脳波と血圧の間には負の相関関係があることが示され,脳波と動脈血酸素飽和度の間には相関関係がないことが示された.

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印象の個人差が少ない音楽を対象として,音楽が生体に与える効果を検討した.まず,あらかじめ選定した9曲を対象として印象測定をSD法により実施した結果,悲しい印象および楽しい印象を与える曲は,印象の個人差が少ないことが確認された.これに対し,リラックスできる曲の印象の個人差は大きかった.次に,個人間の印象のばらつきが最も少ない音楽を対象として,それらを鑑賞前・中・後の生理指標(心電図,脳波,呼吸)と気分の計測を行った.その結果,被験者によらず,印象の異なる音楽によって気分に与える影響が異なること,心電図R-R間隔は音楽鑑賞中に延長すること,R-R間隔は疲労感と相関があることが確認された.

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養生気功の1つである六字訣における中国人、日本人気功師による発声の音声解析を行った。その結果、14.8kHz 以上の高い周波数成分があることが分った。基本周波数は2次に倍音が基本だったが、どちらの六字訣の発声音かにより構造に違いが見られた。さらに、六字訣の気功師による発声音を聴くことによりどのような生理物理的変化が経絡に現れるかを、15人の被験者で単一矩形パルス法により測定した。六字訣による経絡間の順位変化の平均値は系統的な傾向を示した。つまり、これまで六字訣の実践は嘘、呵、呼、〓、吹、〓の6音韻を発声して体に響かせることにより対応した臓器を強化できると考えられてきたが、実際に特定の内臓や経絡に影響すると考えられる。

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We studied the change of the heart rate variability (HRV) in climacteric women by the external Qi (MOA purifying therapy). MOA purifying therapy was administrated to the therapy group (n=25, 51.8±3.3 years) , while the control group (n=25, 55.5±3.8 years) relaxed on the bed. All subjects were climacteric women. The R-R intervals of subjects' electrocardiogram were measured. The HF component and the LF/HF component were analyzed by the Wavelet transformation. As a result, the HF component during administration in the therapy group increased more than that during the relaxed position in the control group, significantly (p<0.005). There was no difference between the LF/HF component of the therapy group and the control group. It was supposed from this result that the administration of MOA purifying therapy activated parasympathetic nerve in the climacteric women. We expect that the application of external Qi, MOA purifying therapy, improves the health of patients with the climacteric syndrome.

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Changes of fever, stiffness and pain by receiving health promotion program with a central focus on MOA purifying therapy are quantified for 103 subjects. Fever and stiffness are digitally quantified by areas determined by drawing on a human body figure using a pen tablet PC. Pain scales are also evaluated subjectively by 10 steps. These indexes and can represent the effect of MOA purifying therapy well.

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近年、可視光は、視覚経路を介して免疫反応に影響を与えることが示唆されてきている。一方、哺乳類以外の脊椎動物では、光が頭蓋骨を透過することが知られてきている。われわれは、8名の健常男性を被験者に、服部を遮光して、前頭部に赤色発光ダイオード光を照射し、末梢血中のナチュラルキラー細胞の活性(NK活性)とその数の指標(CD57^-CD16^+)の変化を調べ、さらにこれらと脳波(α波)の変化との相関性について調べた。その結果、15分間のダイオード光照射により、NK活性の上昇傾向と、CD57^-CD16^+の有意な増加を認めた。また、CD57^-CD16^+の変化と測定後最初の5分間の実効振幅の変化との間には、左右前頭部では相関性を認めたが、左右後頭部では相関性が認められなかった。光は、ヒトにおいても、頭蓋骨を透過して前頭葉を中心にα波の振幅の変化をもたらし、おそらく何らかの神経伝達物質の放出を介して血中のNK細胞数を増加させるのではないかと考えられた。

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本研究はストレスの中でも、精神的ストレスを対象にし、その対処法の一つとして香りを用いたリラックス効果を定量的に検証することが目的である。被験者は健康な成人男性10名を選んだ。生理指標には、心拍数(Heart Rate: HR)、収縮期血圧(Systolic Blood Pressure: SBP)、分泌型免疫グロブリンA(Secretory Immunoglibulin A: SIgA)を測定した。精神心理状態検査には日本語版POMS(感情プロフィール検査)を、ストレス負荷には内田クレペリン検査を25分間行なった。香料(精油)はマジョラムエッセンシャルオイルを使用した。HRとSBPは香り刺激によって有意な低下を示した(P<0.05)。SIgAはコントロール時、香り提示時とともにSIgA濃度が上昇を示した。また、回復20分後では、コントロール時に比し、香り提示の場合に有意な上昇を示した(P<0.05)。本研究ではストレスに対して香りがリラクゼーション効果をもたらすことが示唆された。

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We investigated electroencephalogram (EEG) of brain respiration (BR) trained elementary school students. Five students are BR trained over six months and the other five students are not. We measured EEG from 16 channels during learning activities and calculated a correlation dimension as a nonlinear measure. BR trained students had higher average values of the D2 at Fp2 channel during resting state. When subjects were reading a book, BR trained students had higher average values of D2 at P3 channel. D2 of BR trained students were higher at C4 channel during mental arithmetic, and at F4 channel during finding their way out a maze than normal controls. These results suggest that the brain of BR trained students remained in a more attentive state during learning activities than that of normal controls.

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In this research, we construct Hurst Exponent(HE) model for classification of heartbeat, and making a diagnosis for active treatment of Chinese medical, the basic data of acupuncture treatment is supported by opinions of gross examination, pathological diagnosis, and immunotherapy from obtaining biological signals. We measure the heartbeat through the MCG, 14 healthy and 34 abnormal subjects, heart-deficiency syndrome, ostensibly. As our experiment, the mean values are changed 0.848, 0.755, 0.805, before, during and after acupuncture treatment in case of abnormal subjects, on the other hand, 0.808, 0.798, 0.767 in normal respectively. And then, we prove that HE index variability by acupuncture treatment is different normal controls and abnormal each other, also the acupuncture effect through the paired sample test of healthy and heart-deficiency syndrome subjects.

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Cognitive functions can be detected by heartbeat signals. We have obtained EKG data from neonates in three cognitive states - rest, auditory stimulus and (post)rest states. We have got several statistical properties of the heartbeat signals from the neonates who were used to hear artificial heartbeat signals when they were in their mother's womb. Also, we compared them with those of normal neonates who were not. Auditory stimulus used in this experiment mimics mother's heartbeat signal. All the neonates tested were about 5 months old. There are no significant difference in mean-heart rates and standard deviation of heart rate between two groups and among three states. However the statistical characteristics based on nonlinear dynamics and information theory, which tells the difference between two groups and among three states has been detected.

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Lipiodol, iodized poppy seed oil, is widely used as a contrast media and can be traced by x-ray. Although it was very slow, lipiodol injected to acupoints flowed along the meridian line upward or downward as was suggested by meridian theory. Tracing of injected lipiodol by x-ray revealed that the meridians are a unique morphological entity through which the material flows.

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We isolated duct system from the dermis of rabbit believed to be traditional meridian line. Methylene blue stain shows a bundle of tubules inside the duct. The diameter of the duct and the tubule was about 70 μm and 5-10μm, respectively. Close view showed fine fiber-like striae inside the tubules, through which very narrow lines of small granules of which the size is about 1 μm could be directly observed. These results confirm the identity of isolated duct from the dermis as a Bonghan duct. The cryosection of the ducts showed many lines of the rod shaped nuclei distributed as broken-line stripes, which is also a typical characteristic of Bonghan duct.

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We observed threadlike structure separated partially from peritoneum and connected to the peritoneum or abdominal wall. The corpuscle on the way of the threadlike structure on the organ surface of a rabbit contained 2fm size granules. The study with confocal laser scanning microscope revealed that there were DNA inside of the granule. The granule might be a sort of cell-free DNA, or a naturally existing micro-cell.

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Numerous magnetic devices for magneto-therapy including steady magnets with various forms and sizes are widely produced. In such devices the gradient magnetic fields are combined with needed mechanical vibrations. Magnetic concentration effects occur always in living systems because gradient magnetic fields inside organism are strong enough to cause drifts of cellular complexes and organelles by micron and submicron sizes. In the present article we report on the appearance of super-paramagnetic contaminations inside developing eggs of Artemia shrimps. In developing shrimps eggs ferritin aggregates were observed under weak gradient magnetic fields and dia-para-ferromagnetic transitions were detected. A significant interruption of eggs' developing was revealed in such fields.

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The presence of Bonghan ducts inside the blood vessels of rabbits was reconfirmed in the 1970's by Lee and Fujiwara. Intravascular Bonghan ducts were taken from the vena cava inferior and from the ovarian-vessels by using the methylene-blue staining method. The author proposes that causes of cancers are deeply connected with the pathological condition of the Bonghan system.

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We report on the observation of thread-like structures which run inside the aorta and coronary artery of the swine. Althrough the current knowledge of animal anatomy and physiology does not explain such structure, it is found that these intravascular thread-like structures were attached with clotting mass in the left ventricle of the heart. These structures were running along the inside of the aorta and coronary artery. These structures were semitransparent when it was washed with saline. The thickness of these structures varied from 100 to 300um. We considered that these structures were morphologically similar with intravascular Bonghan duct (IBHD). More extensive investigation is necessary to elucidate whether intravascular thread-like structure are truly Bonghan duct or material of clot.

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