Anthocyanin pigments (ANs) were extracted from purple yam (UBE) and sweet potato (SP), and compared them with those of marketed AN obtained from grapes (GJ), purple corns (PC) and red cabbages (RC) Each AN was purified by basic lead acetate, precipitating from ether and ion exchange resin.
ANs extracted from UBE were separated into PA, PB and PC with thin layer chromatography. Then it was repeatedly treated with basic lead acetate and precipitated from ether to give three purified ANs.
1) pH : The UBE AN showed the same color as that of Red Colorant No. 2 at pH values of 2. 2 and 3. 2 and showed no decoloration when allowed to stand for a while. It turned to pale red at pH 4. 8 and further changed from purple to blue at pH 7. 0 to 8. 0.
2) Stability to heat : UBE was the most stable to heat among these AN pigments. On the other hand, SP showed the lowest colorant remaining ratio and was more unstable than other.
3) Stability to light : The colorant remaining ratio of each AN was low,
i. e., lower than 60 %. UBE showed a particularly lower colorants remaining ratio compared with others while SP was relatively stable.
4) Stability to oxygen : No effect of oxygen was observed on colorant stability.
5) Effects of heat, light and oxygen on three purified ANs were examined.
The ANs decomposed with an increase in the heat and with the elapse of time. These three ANs showed almost the same colorant remaining ratios, although UBE used for comparison was more stable than each one of these purified ANs. UBE and three purified ANs were unstable to light. No effect of oxygen on three purified ANs was observed.
View full abstract