巻号一覧
34 巻, 3 号
選択された号の論文の8件中1～8を表示しています
- |<
- <
- 1
- >
- >|
-
伊藤 宜則, 栗田 秀樹, 落合 昭博, 大谷 元彦1979 年 34 巻 3 号 p. 465-473
発行日: 1979/08/30
公開日: 2009/02/17
ジャーナル フリーIn the physical screening for the clinical diagnosis of hyperlipemia, total cholesterol values are used rather than total serum lipid values.
A simplified method for total serum lipid determination using the sulfo-phospho-vanillin reaction has recently been employed in clinical laboratories. The sum of total cholesterol, triglyceride, and phospholipid was proposed by Cheek, summation method 1, and this method is used to determine the total serum lipid values for the clinical test.
We evaluated the total serum lipid values in hyperlipemia by the sulfo-phospho-vanillin reaction method, summation method 1, and another method, summation method 2, in which each value for total cholesterol, triglyceride, and phospholipid are summed.
Results:
1) The sulfo-phospho-vanillin reaction method shows the specific reaction to higher unsaturated fatty acids but not to saturated fatty acids. Total serum lipid values measured by gravimetry and oxidometry show good correlations when this method is used.
2) The mean value of total serum lipid determined by summation method 1 is in good agreement with the values from gravimetry and oxidometry.
3) The values obtained by summation method 2 are about 15% lower than those obtained by the standard method, gravimetry.
4) In the diagnosis of hyperlipemia the sensitivity is 72.3% in the sulfo-phospho-vanillin reaction method, 71.1% in summation method 1, and 78.0% in summation method 2.
We concluded that the values determined by the sulfo-phospho-vanillin reaction method, and the lipid-summation methods proposed by Cheek and developed by us are useful for the physical screening of hyperlipemia.抄録全体を表示PDF形式でダウンロード (1231K) -
古武 弥三, 服部 雅康1979 年 34 巻 3 号 p. 474-483
発行日: 1979/08/30
公開日: 2009/02/17
ジャーナル フリーYahito Kotake1, 2) has shown that rats continuously fed a high fat and high protein diet contracted diabetes and excreted xanthurenic acid (XA). His coworker has taken urine samples from 23 human diabetics and concentrated them. After separating the urine components with solvent on paper chromatography, he identified XA with diazo, by Millon's reaction and by the ferric sulfate test. XA was detected in most diabetic urine, but not in normal urine, which suggests that there is a significant relationship between XA and diabetes. But the existence of diabetics who do not excrete XA in their urine also has been noticed.
Thus, we needed to clarify clinically the rate at which usual diabetic patients excrete XA. XA is known to be produced when there is a deficiency of vitamin B6. Therefore when patients excrete XA in their urine, the administration of vitamin B6 may have a therapeutic effect.
In cases of mild or middle grade diabetics, only 30-50% of the patients excreted XA in their urine, but if they were loaded with tryptophan the ratio would increase. We believe that there are some diabetics whose tryptophan metabolism is distorted, since their excretion rate of XA in urine is much higher than that of healthy individuals. Whether these diabetic patients have a shortage of vitamin B6 or there is a decrease in the change of vitamin B6 to the active form, pyridoxal phosphate, is discussed.抄録全体を表示PDF形式でダウンロード (1200K) -
久野 由基一, 根本 信子, 森田 博行, 小池 重夫1979 年 34 巻 3 号 p. 484-487
発行日: 1979/08/30
公開日: 2009/02/17
ジャーナル フリーAn elevated plasma sialic acid concentration was found in rabbits and rats associated with triglyceridemia induced by cobalt chloride administration. Acrylamide gel electrophoresis of the serum from rabbits treated with cobalt chloride showed augmentation of the band corresponding to haptoglobin.
Serum haptoglobin levels were determined with cellulose acetate electrophoresis, and were found to increase in cobalt treated rabbits and rats.抄録全体を表示PDF形式でダウンロード (1817K) -
中川 正祥1979 年 34 巻 3 号 p. 488-495
発行日: 1979/08/30
公開日: 2009/02/17
ジャーナル フリー比較的弱い磁場が, 生体に何らかの作用を及ぼし得るかどうかを検出するために, CFW系マウスを用いて再生産試験を行った。マウスを交配の初期から300 Oeと800 Oeとの磁場へ入れ, 生れた仔は8週齢まで磁場内で飼育を続けた。次の世代からは磁場外で交配を続けて以下3世代まで観察した。800 Oeの磁場では妊娠期間が伸び, 妊娠率が低下したが, 生存率と離乳率の減少は有意ではなかった。また生出産仔数, 生下時体重と生長にも対照に比べて低下が認められた。300 Oeの磁場でも生出産仔数, 生下時体重の減少が見られ, また生長もやや遅延した。磁場内では雄に比べ雌の生出産仔数が低下するようだったが, 全体として分析を行うと有意ではなかった。磁場の作用は, 磁場の外で交配させた後の世代までも影響が残るように思われ, ことに800 Oe群で著明だった。
既に報告されているアメリカとソ連の磁場に関する安全基準についていくらかの見解を述べた。抄録全体を表示PDF形式でダウンロード (2640K) -
森田 博行, 久野 由基一1979 年 34 巻 3 号 p. 496-506
発行日: 1979/08/30
公開日: 2009/02/17
ジャーナル フリー正常食, 3日間空腹, 3日間空腹+塩化コバルト注射の3群の家兎とラットの肝遊離細胞について, 空腹状態, あるいはコバルト注射が遊離脂肪酸の代謝にどのように影響するかを観察した。
空腹ラットの肝細胞では, 標識パルミチン酸のエステル化は正常食ラットに比べて著しく減少したが, ケトン体への酸化の転換総量は増加した。このエステル化の減少はトリグリセライド (TG) と燐脂質の生成の減少によるが, とりわけTGの生成の減少が大きく影響していた。しかしケトン体の生成は空腹によって亢進した。他方, 家兎の肝細胞では脂肪酸のエステル化と酸化は空腹によって殆んど影響されなかった。
コバルト注射家兎の肝細胞では標識したパルミチン酸のTGへのエステル化が空腹家兎に比べて亢進した。しかし, コバルト注射ラットの肝細胞ではTGへのエステル化は空腹ラットと差がなかったが, 燐脂質の生成は, 促進し, ケトン体生成は有意に減少した。
空腹の家兎では血中のケトン体は顕著に増加したが, コバルト注射によってその増加は抑制された。この家兎の血中のケトン体の変動は, 空腹ラットの肝細胞におけるケトン体生成の増加とコバルト注射によるその増加の抑制というラットの肝細胞の挙動にむしろ近かった。
3群の動物の遊離肝細胞を浮遊した培養液にコバルトを加えたところ標識したパルミチン酸のエステル化と酸化の度合には, 添加しないものに比べて, なんらの差がみられなかった。
培養液にコバルトを添加すると3群の遊離肝細胞からのTGの放出が抑制されたが, アセト酢酸の放出は阻害されなかった。即ち, 培養液にコバルトを添加しても遊離肝細胞のTGの生成とアセト酢酸の放出には影響しなかったがTGの放出は完全に阻害された。抄録全体を表示PDF形式でダウンロード (1305K) -
I 細胞分裂に及ぼす影響福島 晋一, 塩田 千代, 小川 博, 笹川 祐成1979 年 34 巻 3 号 p. 507-511
発行日: 1979/08/30
公開日: 2009/02/17
ジャーナル フリーThe effects of heavy metal compounds (MnCl2⋅Mn(CH3COO)2⋅PbCl2⋅Pb(CH3COO)2⋅CdCl2⋅Cd (CH3COO)2⋅HgCl2⋅K2Cr2O7) on cell division in Paramecium tetraurelia were investigated. Cells used in the present experiments had previously induced autogamy; they were vigorous and divided about 8 times in 40 hours at 25°C. In a cell population that had been reproduced from a cell isolated two days before, nine cells (control group) were isolated and placed in normal medium and groups of six cells (experimental groups) were isolated and placed in media that contained heavy metals at different concentrations. Cultures were then incubated at 25°C for two days, then the number of cell divisions was counted.
The number of cell divisions was gradually reduced at concentrations of manganese more than 100μM, of lead more than 30μM, of cadmium more than 2μM, of mercury more than 2μM, and of chromium (VI) more than 10μM. These concentrations of Pb, Cr (VI) and Cd are nearly the same to as the standard concentrations allowed in drainage in Japan. This suggests that the cell of paramecium can be used to test the toxicity of heavy metals.抄録全体を表示PDF形式でダウンロード (591K) -
食道・胃・肺がんの市郡別死亡分布を例として大野 良之, 青木 国雄, 青木 伸雄, 大谷 元彦1979 年 34 巻 3 号 p. 512-520
発行日: 1979/08/30
公開日: 2009/02/17
ジャーナル フリーIn geographic epidemiology, distributions of the categorized mortality or morbidity rates are visualized on maps. Visual study, however, by no means proves whether geographic aggregations occur by chance alone. We have developed a test of significance to assess the deviation from chance expectations of geographic patterns actually observed and have described it in this paper. The significance is tested by comparing the observed number of adjacent areas having concordant category pairs with the number obtained by a simulation based on the Monte Carlo method.
The test was applied to illustrations of geographic patterns of the categorized mortality from esophageal, stomach, and lung cancers in Japan, 1969-1971. For esophageal cancer, high mortality was significantly clustered in both sexes, and low mortality in males. For stomach cancer, both high and low mortality was significantly aggregated in both sexes. Low lung cancer mortality showed significant geographic aggregations in males.
Some prerequisites for the proper use of the test, the characteristics of the random numbers generated, the changeable significance levels, and the methodological alternative are discussed. The test is applicable not only to geographic epidemiology, but also to other scientific fields where an investigator is concerned with geographic clusters of variables or events.抄録全体を表示PDF形式でダウンロード (5964K) -
白石 信尚1979 年 34 巻 3 号 p. 521-549
発行日: 1979/08/30
公開日: 2009/02/17
ジャーナル フリーPDF形式でダウンロード (5406K)
- |<
- <
- 1
- >
- >|